We narrowed to 117,439 results for: plasmid
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Plasmid#62987PurposeCas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthSpCas9n-2A-Puro
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianMutationCas9 D10A nickase mutantAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-DIO-hM3D(Gq)-mCherry (AAV PHP.eB)
Viral prep#44361-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV-hSyn-DIO-hM3D(Gq)-mCherry (#44361). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM3D(Gq)-mCherry plasmid DNA. Syn-driven, Cre-dependent, hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV were produced with the PHP.eB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmCherry (Cre-dependent)Available sinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-hM4D(Gi)-mCherry (AAV Retrograde)
Viral prep#50475-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-hSyn-hM4D(Gi)-mCherry (#50475). In addition to the viral particles, you will also receive purified pAAV-hSyn-hM4D(Gi)-mCherry plasmid DNA. CNO-induced neuronal silencing. Synapsin-driven with mCherry reporter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmCherryAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-CAG-hChR2-H134R-tdTomato (AAV Retrograde)
Viral prep#28017-AAVrgPurposeReady-to-use AAV Retrograde particles produced from AAV-CAG-hChR2-H134R-tdTomato (#28017). In addition to the viral particles, you will also receive purified AAV-CAG-hChR2-H134R-tdTomato plasmid DNA. Humanized channelrhodopsin H134R mutant fused to tdTomato, under the control of the CAG promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPublicationPromoterCAGTagstdTomatoAvailable sinceJune 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
BJ5183 cells
Bacterial strain#16398DepositorBacterial resistanceStreptomycinSpeciesEscherichia coliAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCas9
Plasmid#42876PurposeBacterial expression of Cas9 nuclease, tracrRNA and crRNA guideDepositorInserttracr/Cas9
UseCRISPR; E.coliExpressionBacterialAvailable sinceFeb. 26, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCas9
Plasmid#42876PurposeBacterial expression of Cas9 nuclease, tracrRNA and crRNA guideDepositorInserttracr/Cas9
UseCRISPR; E.coliExpressionBacterialAvailable sinceFeb. 26, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mini-pHelper-1.0
Plasmid#230933PurposeThe mini version (8.4 kb) of Ad helper plasmid to provide E2A, E4orf6, and VA RNA for AAV productionDepositorTypeEmpty backboneUseAAVAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
mini-pHelper-1.0
Plasmid#230933PurposeThe mini version (8.4 kb) of Ad helper plasmid to provide E2A, E4orf6, and VA RNA for AAV productionDepositorTypeEmpty backboneUseAAVAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1-GfaABC1D-mCherry-hPMCA2w/b (AAV5)
Viral prep#111568-AAV5PurposeReady-to-use AAV5 particles produced from pZac2.1-GfaABC1D-mCherry-hPMCA2w/b (#111568). In addition to the viral particles, you will also receive purified pZac2.1-GfaABC1D-mCherry-hPMCA2w/b plasmid DNA. GfaABCD1-driven, expression of mCherry fused plasma membrane calcium pump hPMCA2w/b. These AAV preparations are suitable purity for injection into animals.DepositorTagsmCherryAvailable sinceFeb. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
Dual-sgRNA_hU6-mU6
Plasmid#154194PurposeLentiviral expression plasmid for two sgRNAs from a hU6 and a mU6 promoter. The cloning site design allows a one-step cloning strategy of both sgRNAs. Expresses a Thy1.1 marker.DepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Dual-sgRNA_hU6-mU6
Plasmid#154194PurposeLentiviral expression plasmid for two sgRNAs from a hU6 and a mU6 promoter. The cloning site design allows a one-step cloning strategy of both sgRNAs. Expresses a Thy1.1 marker.DepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry
Plasmid#41863PurposeThis plasmid is used to reconstitute the complete Type II CRISPR system from S. pyogenes and contains the host factor RNaseIII and tracrRNA.DepositorPublicationInsertU6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry
UseCRISPR and LentiviralExpressionMammalianAvailable sinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry
Plasmid#41863PurposeThis plasmid is used to reconstitute the complete Type II CRISPR system from S. pyogenes and contains the host factor RNaseIII and tracrRNA.DepositorPublicationInsertU6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry
UseCRISPR and LentiviralExpressionMammalianAvailable sinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GreenGate
Plasmid kit#1000000036PurposePlasmids for rapidly assembling plant transformation constructs, based on the Golden Gate method.DepositorApplicationCloning and Synthetic BiologyVector typePlant ExpressionCloning typeGolden Gate (GreenGate)Available sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
8xGTIIC-luciferase
Plasmid#34615PurposeYAP/TAZ-responsive synthetic promoter driving luciferase expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsynthetic TEAD luciferase reporter
UseLuciferaseAvailable sinceMarch 16, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
8xGTIIC-luciferase
Plasmid#34615PurposeYAP/TAZ-responsive synthetic promoter driving luciferase expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsynthetic TEAD luciferase reporter
UseLuciferaseAvailable sinceMarch 16, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.CAG.Flex.NES-jRGECO1a.WPRE.SV40 (AAV1)
Viral prep#100852-AAV1PurposeReady-to-use AAV1 particles produced from pAAV.CAG.Flex.NES-jRGECO1a.WPRE.SV40 (#100852). In addition to the viral particles, you will also receive purified pAAV.CAG.Flex.NES-jRGECO1a.WPRE.SV40 plasmid DNA. CAG-driven, Cre-dependent jRGECO1a calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGAvailable sinceJuly 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSL1142 (pSPIN, pBBR1 backbone)
Plasmid#160730PurposeSingle-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pBBR1 backbone.DepositorInsertVchCAST proteins, crRNA, and donor DNA
ExpressionBacterialPromoterJ23119Available sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSL1142 (pSPIN, pBBR1 backbone)
Plasmid#160730PurposeSingle-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pBBR1 backbone.DepositorInsertVchCAST proteins, crRNA, and donor DNA
ExpressionBacterialPromoterJ23119Available sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by