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pLX_317-C7orf26v1

Plasmid

#178537

Purpose

Subcloned C7orf26v1 (ENST00000344417, Origene, RC219786L4) into pLX_317 backbone

Depositor

William Hahn

Article

Pan et al Cell Syst. 2022 Jan 21. pii: S2405-4712(

Insert

C7orf26v1 (INTS15 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TLR8-YFP

Plasmid

#13024

Depositor

Doug Golenbock

Insert

Toll-Like Receptor 8 (TLR8 Human)

Tags

YFP

Expression

Mammalian

Available Since

Nov. 3, 2006

Availability

Academic Institutions and Nonprofits only

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pEGFP-N3/hArf1(WT)-EGFP

Plasmid

#79413

Purpose

Expresses C-terminally EGFP-tagged Arf1(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Kondo et al Cell Struct Funct. 2012;37(2):141-54. Ep

Insert

Arf1 (ARF1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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CiDr-VSP L223F-mCherry pcDNA3.1 (eVSP)

Plasmid

#140892

Purpose

Expresses modified Danio rerio VSP with enhanced phosphatase activity fused with mCherry in mammalian cells.

Depositor

Yasushi Okamura

Article

Kawanabe et al J Gen Physiol. 2020 May 4;152(5). pii: 1

Insert

CiDr-VSP L223F-mCherry (tpte, vsp Ciona intestinalis, Zebrafish)

Tags

mCherry

Expression

Mammalian

Mutation

changed 223L to F in Dr-VSP (zebrafish TPTE)

Available Since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-FCHO1

Plasmid

#196910

Purpose

visualization of clathrin-coaed vesicle formation in living mammalian cells

Depositor

Kenji Sugimoto

Article

Sakaushi et al Biosci Biotechnol Biochem. 2007 Jul;71(7

Insert

FCHO1 (FCHO1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223_HIF1A_WT

Plasmid

#82129

Purpose

Gateway Donor vector containing HIF1A , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HIF1A (HIF1A Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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SM265_EF1A-dHyperLbCas12a-KRAB-T2A-EGFP

Plasmid

#244204

Purpose

Mammalian expression of dHyperLbCas12a-KRAB with EGFP selectable marker

Depositor

Tim Reddy

Article

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Insert

dHyperLbCas12a-KRAB

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pJMP3654

Plasmid

#222349

Purpose

Expression vector with GFP expressed under PabstBR promoter, kanR marker, and replicates in A. baumannii and E. coli.

Depositor

Jason Peters

Article

Bacon et al Microbiol Spectr. 2024 Nov 5;12(11):e013

Insert

sfGFP

Expression

Bacterial

Promoter

PabstBR

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-EGFPL10a

Plasmid

#98747

Purpose

Cre-dependently expresses the fusion protein EGFPL10a

Depositor

Nathaniel Heintz, Alexander Nectow, Eric Schmidt

Article

Nectow et al Cell Rep. 2017 Apr 18;19(3):655-667. doi

Has Service

AAV Retrograde and AAV5

Insert

EGFPL10a

Use

AAV and Cre/Lox

Tags

GFP

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-mCherry (AAV2)

Viral Prep

#114470-AAV2

Purpose

Ready-to-use AAV2 particles produced from pAAV-Ef1a-mCherry (#114470). In addition to the viral particles, you will also receive purified pAAV-Ef1a-mCherry plasmid DNA. EF1a-driven mCherry expression. These AAV preparations are suitable purity for injection into animals.

Depositor

Karl Deisseroth

Promoter

EF1a

Tags

mCherry

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only
pHAGE-PIK3CA-E542K

Plasmid

#116479

Purpose

Lentiviral expression of PIK3CA E542K

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CA (PIK3CA Human)

Use

Lentiviral

Mutation

E542K

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pMXs-mPKR-FLAG-IG

Plasmid

#101792

Purpose

Retroviral vector encoding C-terminally FLAG tagged mouse PKR

Depositor

Seiichi Oyadomari

Article

Taniuchi et al Sci Rep. 2016 Sep 16;6:32886. doi: 10.10

Insert

Pkr (Eif2ak2 Mouse)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Promoter

LTR

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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EF1a-EGFP (no signal control)

Plasmid

#170167

Purpose

Mammalian Expression of no signal control

Depositor

Christopher Moxham

Type

Empty backbone

Expression

Mammalian

Mutation

n/a

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pPEgRNA

Plasmid

#172716

Purpose

Delivery of guide RNA for prime editing

Depositor

Tilmann Weber

Article

Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

Insert

gRNA

Expression

Bacterial

Available Since

Sept. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.Flex.GCaMP6s.WPRE.SV40

Plasmid

#100842

Purpose

AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from CAG promoter

Depositor

Douglas Kim, GENIE Project

Article

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Has Service

AAV1, AAV5, and AAV9

Insert

GCaMP6s

Use

AAV

Expression

Mammalian

Mutation

GCaMP3-K78H T302L R303P D380Y T381R S383T R392G

Promoter

CAG

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-GFP-SFPQ-IRES-mCherry

Plasmid

#166950

Purpose

Lentiviral plasmid expressing GFP-tagged SFPQ protein with IRES-mCherry from the EF1a promoter

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

SFPQ (SFPQ Human)

Use

Lentiviral

Tags

GFP and mCherry

Promoter

eF1a

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-dCas9-VPR-pgk-hph

Plasmid

#233066

Purpose

Piggybac CRISPRa construct

Depositor

Markus Landthaler

Article

Vucicevic et al Cell Genom. 2025 Oct 8;5(10):100984. doi

Insert

dCas9-VPR

Use

CRISPR

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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plex_306-ccdB-g418

Plasmid

#201133

Purpose

pLEX_306 plasmid with g418 marker

Depositor

Alejandro Chavez

Type

Empty backbone

Expression

Mammalian

Promoter

SV40

Available Since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsTAB182_K

Plasmid

#146774

Purpose

Mammalian Expression of HsTAB182

Depositor

Elisa Izaurralde

Article

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

HsTAB182 (TNKS1BP1 Human)

Expression

Mammalian

Mutation

2 silent mutations compared to the sequence given…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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Rtn4a-GFP

Plasmid

#61807

Purpose

Human Rtn4a fluorescently tagged with GFP on the C-terminus for mammalian expression

Depositor

Gia Voeltz

Article

Shibata et al J Biol Chem. 2008 Jul 4. 283(27):18892-9

Insert

Rtn4a (RTN4 Human)

Tags

AcGFP

Expression

Mammalian

Mutation

Rtn4a Stop Codon removed

Promoter

CMV

Available Since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

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