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pRS313 - prp8 T1865K

Plasmid

#90968

Purpose

yeast vector

Depositor

Christine Guthrie

Article

Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

Insert

PRP8 (PRP8 Budding Yeast)

Expression

Yeast

Mutation

T1865K

Availability

Academic Institutions and Nonprofits only

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pRS313 - prp8 A1871E

Plasmid

#90969

Purpose

yeast vector

Depositor

Christine Guthrie

Article

Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

Insert

PRP8 (PRP8 Budding Yeast)

Expression

Yeast

Mutation

A1871E

Availability

Academic Institutions and Nonprofits only

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pRS313 - prp8 T1872E

Plasmid

#90970

Purpose

yeast vector

Depositor

Christine Guthrie

Article

Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

Insert

PRP8 (PRP8 Budding Yeast)

Expression

Yeast

Mutation

T1872E

Availability

Academic Institutions and Nonprofits only

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pRS313 - prp8 T1861P

Plasmid

#90971

Purpose

yeast vector

Depositor

Christine Guthrie

Article

Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

Insert

PRP8 (PRP8 Budding Yeast)

Expression

Yeast

Mutation

T1861P

Availability

Academic Institutions and Nonprofits only

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pRS313 - prp8 V1862Y

Plasmid

#90972

Purpose

yeast vector

Depositor

Christine Guthrie

Article

Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

Insert

PRP8 (PRP8 Budding Yeast)

Expression

Yeast

Mutation

V1862Y

Availability

Academic Institutions and Nonprofits only

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pRS313 - prp8 H1863E

Plasmid

#90973

Purpose

yeast vector

Depositor

Christine Guthrie

Article

Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

Insert

PRP8 (PRP8 Budding Yeast)

Expression

Yeast

Mutation

H1863E

Availability

Academic Institutions and Nonprofits only

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pRS313 - prp8 K1864E

Plasmid

#90974

Purpose

yeast vector

Depositor

Christine Guthrie

Article

Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

Insert

PRP8 (PRP8 Budding Yeast)

Expression

Yeast

Mutation

K1864E

Availability

Academic Institutions and Nonprofits only

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pRS313 - prp8 N1869D

Plasmid

#90975

Purpose

yeast vector

Depositor

Christine Guthrie

Article

Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

Insert

PRP8 (PRP8 Budding Yeast)

Expression

Yeast

Mutation

N1869D

Availability

Academic Institutions and Nonprofits only

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pRS313 - prp8 V1870N

Plasmid

#90976

Purpose

yeast vector

Depositor

Christine Guthrie

Article

Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

Insert

PRP8 (PRP8 Budding Yeast)

Expression

Yeast

Mutation

V1870N

Availability

Academic Institutions and Nonprofits only

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pRS313 - prp8 I1875T

Plasmid

#90977

Purpose

yeast vector

Depositor

Christine Guthrie

Article

Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

Insert

PRP8 (PRP8 Budding Yeast)

Expression

Yeast

Mutation

I1875T

Availability

Academic Institutions and Nonprofits only

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pAV10

Plasmid

#63213

Purpose

pUC19 backbone (Amp), contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. NotI or FseI sites for TU digest.

Depositor

Jef Boeke

Article

Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Availability

Academic Institutions and Nonprofits only

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HM3C NaVSp1 pET24b

Plasmid

#128088

Purpose

Bacterial expression vector for NaVSp1 bacterial sodium channel. It expresses for HIStag-MBP-3C-channel protein

Depositor

Dan Minor

Article

Shaya et al Proc Natl Acad Sci U S A. 2011 Jul 26;10

Insert

NaVSp1p

Tags

3C Cleavage, His Tag, and MBP

Expression

Bacterial

Availability

Academic Institutions and Nonprofits only

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pET41a iGlum

Plasmid

#119829

Purpose

Production of recombinant iGluSnFR R24K medium affinity variant, a single-wavelength glutamate indicator, in E. coli

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGlum

Tags

GST

Expression

Bacterial

Mutation

R24K

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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GFP-HDEL

Plasmid

#129098

Purpose

For labeling endoplasmic reticulum (ER) in S. cerevisiae with GFP-HDEL

Depositor

Wei-Lih Lee

Article

Omer et al Elife. 2018 Aug 7;7. pii: 36745. doi: 10

Insert

TEF1p-SS-3xGlyAla-GFP-HDEL

Expression

Yeast

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pFPV25.1

Plasmid

#20668

Purpose

S. typhimurium rpsM promoter driving gfpmut3 expression in bacteria. Promoter is not regulated by acidic conditions.

Depositor

Raphael Valdivia

Article

Valdivia et al Mol Microbiol. 1996 Oct . 22(2):367-78.

Type

Empty backbone

Tags

GFP mut3

Expression

Bacterial

Available Since

March 5, 2009

Availability

Academic Institutions and Nonprofits only

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pPTbsr

Plasmid

#117696

Purpose

Blasticidin-S resistance Vector for Phaeodactylum tricornutum

Depositor

Peter Kroth

Article

Buck et al PeerJ. 2018 Nov 14;6:e5884. doi: 10.7717

Type

Empty backbone

Use

Vector for phaeodactylum tricornutum

Promoter

fcpB

Available Since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pYL112-19

Plasmid

#15459

Depositor

Chia Lee

Article

Luong et al J Microbiol Methods. 2007 Jul . 70(1):18

Insert

S. aureus phage L54a integrase

Use

S.aureus-e. coli shuttle vector

Available Since

Oct. 9, 2007

Availability

Academic Institutions and Nonprofits only
pCoofy40

Plasmid

#44006

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

His10-Stop-OneStrep-Stop-S-Stop-CBP-Stop-HPC4-Sto…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pAGM18012

Plasmid

#104657

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

N terminal GST-Tag

Use

Synthetic Biology

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI

Plasmid

#48975

Purpose

for Cre-lox cassette exchange of untagged gene sequences into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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