We narrowed to 30,758 results for: PLE;
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Plasmid#100722PurposeExpression of human Pim1 kinase domain in E.coliDepositorInsertPim1 (PIM1 Human)
TagsHisExpressionBacterialMutationResidues 29-313 of isoform 2PromoterT7 promotorAvailable SinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-TO-HA-SH3BP1 R312A (RNAi-res)
Plasmid#136646PurposeExpression of SH3BP1R12A in mammalian cells; resistant to siRNA targeting GAUGACAGCCACCCACUUCDepositorInsertSH3BP1R312A (RNAi resistant) (SH3BP1 Human)
TagsHA (MQDLPGNDNSTAG)ExpressionMammalianMutationGATGACAGCCACCCACTTC changed to AATGACAGCAACACACTT…Available SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
sgLacZs
Plasmid#171185PurposeExpresses sgRNADepositorInserthU6-sgLacZ1-hU6-sgLacZ2
UseAAVTagsmCherryPromoterU6, hSynAvailable SinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO-TtAgocatdead
Plasmid#210322PurposeBacterial expression of the His- and SUMO-tagged catalytically inactive Argonaute protein of Thermus thermophilusDepositorInsertTtAgo (TT_P0026 Thermus thermophilus)
Tags6x His tag and SUMO tagExpressionBacterialPromoterT7Available SinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2089 - [1-2] ENTR - Tag - SNAP(PATC, no_atg, no_stop)
Plasmid#159874PurposeThree-fragment Gateway compatible protein tag for expression in C. elegansDepositorInsert[1-2] ENTR - Tag - SNAP(PATC, no_atg, no_stop)
ExpressionWormMutationNot applicableAvailable SinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR-v2-Blast-Puro
Plasmid#167186PurposeLentiviral expression of sgRNA with blasticidin and puromycin resistance genesDepositorInsertBlasticidin S deaminase
UseCRISPR and LentiviralExpressionMammalianAvailable SinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR MCS L1-R5
Plasmid#62084PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a multiple cloning site. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseMule gateway entry vectorExpressionMammalianAvailable SinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKD280
Plasmid#125071PurposeExpression of SO_4387 under PtacDepositorUseSynthetic BiologyExpressionBacterialMutationS286L- Please see depositor comments belowAvailable SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAYC
Plasmid#62657PurposeMammalian expression of iCre recombinase from the broadly active CAG promoter/enhancerDepositorInsertiCre
ExpressionMammalianAvailable SinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 WT MYOC eGLuc2 FLAG
Plasmid#192820PurposeExpresses wild-type myocilin with a C-terminal enhanced Gaussia luciferase and FLAG tagDepositorInsertmyocilin (MYOC Human)
TagsFLAG and enhanced Gaussia luciferase (M43I/M110I)ExpressionMammalianPromoterCMVAvailable SinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-blueCKAR(T/A)
Plasmid#118473PurposeNegative-control mutant for blueCKAR biosensor.DepositorInsertblueCKAR(T/A)
Tags6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationContains Thr-to-Ala mutation in substrate sequenc…PromoterCMVAvailable SinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRII-TOPO CMV-cGFP-SV40 Poly(A) Sense
Plasmid#46836PurposeExpress cGFP transcript ending in a poly(A) tail [using the SV40 poly(A) signal]DepositorInsertCoral Green Fluorescent Protein (cGFP)
ExpressionMammalianPromoterCMVAvailable SinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pmcsg7-mCRT(WT)
Plasmid#83505PurposeBacterial expression of CalreticulinDepositorAvailable SinceOct. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
P[acman]-Omega2
Plasmid#165861PurposeOmega2 Basic cloning vector GB2.0 compatible P[acman]-derived backbone. Allows for copy induction from low to high using AutoFOS or similar. Requires EPI300 bacterial strain (DH10B Derived) or similar. uses blue-white screeningDepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1/GgVcl 1-851 A50I mutant
Plasmid#46269DepositorInsertVcl 1-851 A50I mutation (VCL Chicken)
TagsEGFPExpressionMammalianMutation1-851 (Vh) head domain, A50I mutationPromoterCMVAvailable SinceJuly 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGAD-Atg17
Plasmid#121386PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
ExpressionYeastPromoterADH1 promoterAvailable SinceMarch 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR_L1-BirA(R118G)-NES-YFP-STOP-L2
Plasmid#127357Purposegateway entry vector for expressing cytosolic BirA* (R118G)-YFP under a promoter of choiceDepositorInsertBirA* (R118G mutant)
UseGateway entry vectorTagsGS linker, NES, V5, and YFPMutationR118GPromoterno promoterAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/HP + AUG-nLuc-3XFLAG
Plasmid#127322PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with hairpin in 5' UTRDepositorInsertnanoLuciferase
Tags3xFLAGExpressionMammalianPromoterCMVAvailable SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag 3B/WT myc-M87
Plasmid#87722PurposeExpresses WT myc-M87 spastin isoform in mammalian cellsDepositorInsertSPAST (SPAST Human)
TagsMycExpressionMammalianMutationM87 isoform (deletion of nt 1-490 - 5'UTR an…PromoterCMVAvailable SinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2300 - [1-2] ENTR - Fluor - ce-Dendra2(1 intron, syntrons(3), no_atg, no_stop)
Plasmid#159869PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ce-Dendra2(1 intron, syntrons(3), no_atg, no_stop)
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only