We narrowed to 16,291 results for: grna

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p-mCherry

Plasmid

#249067

Purpose

Expresses nucleus-localized mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Article

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

mCherry

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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p-mCherry-enCas9(D10A)

Plasmid

#249063

Purpose

Expresses nucleus-localized enCas9 (D10A) fused to mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Article

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

enCas9

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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p305N-5

Plasmid

#246288

Purpose

Evaluation of AtU6.1m2 promoter (Pol III promoter) sequence variation for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter line

Depositor

Chung-Jui Tsai

Article

Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294

Insert

AtU6.1m2 promoter

Use

CRISPR

Expression

Plant

Mutation

A-to-C (-63 from TSS) and G-to-T (-57) within USE

Promoter

AtU6.1m2 promoter

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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p-att-ef1a-MS2-RecT-dCas9-BSD

Plasmid

#226108

Purpose

Using ef-1a promoter, expresses dCas9 and RecT protein that intended to be tethered via MS2 gRNA hairpin to dCas9, and Blasticidin selection

Depositor

George Church

Article

Oliynyk et al Commun Biol 8 (2025). https://doi.org/10

Insert

BSD

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pGMF1-M

Plasmid

#242203

Purpose

OsU6-2p::sgRNA scaffold in L1 vector backbone, for subcloning of 1st 20 bp target sequence. For use in monocots.

Depositor

Kate Creasey Krainer

Article

Abate et al bioRxiv 2025.11.09.687425

Insert

Level1 OsU6-2p::sgRNA1 for subcloning of 20 bp target sequence

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

OsU6-2p

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pGMF2-M

Plasmid

#242204

Purpose

OsU6-2p::sgRNA scaffold in L1 vector backbone, for subcloning of 2nd 20 bp target sequence. For use in monocots.

Depositor

Kate Creasey Krainer

Article

Abate et al bioRxiv 2025.11.09.687425

Insert

Level1 OsU6-2p::sgRNA2 for subcloning of 20 bp target sequence

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

OsU6-2p

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pGMF1-D

Plasmid

#242205

Purpose

AtU6-26p::sgRNA scaffold in L1 vector backbone, for subcloning of 1st 20 bp target sequence. For use in dicots.

Depositor

Kate Creasey Krainer

Article

Abate et al bioRxiv 2025.11.09.687425

Insert

Level1 AtU6-26p::sgRNA1 for subcloning of 20 bp target sequence

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

AtU6-26p

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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EDCPV PCSK5_sg09

Plasmid

#232455

Purpose

Lentiviral, CRISPR sgRNA for PCSK5

Depositor

Kevin Janes

Article

Marohl et al Mol Cancer Res. 2025 Oct 28. doi: 10.115

Insert

PCSK5 sgRNA (PCSK5 Human)

Use

CRISPR and Lentiviral

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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p305N-30

Plasmid

#246313

Purpose

Evaluation of PtU6.2c3 promoter (Pol III promoter) sequence variation for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter line

Depositor

Chung-Jui Tsai

Article

Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294

Insert

PtU6.2c3 promoter

Use

CRISPR

Expression

Plant

Mutation

deletion: -T (-29 from TSS) within TATA

Promoter

PtU6.2c3 promoter

Available Since

Oct. 30, 2025

Availability

Academic Institutions and Nonprofits only

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p305N-8

Plasmid

#246291

Purpose

Evaluation of AtU6.29c14 promoter (Pol III promoter) sequence variation for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter line

Depositor

Chung-Jui Tsai

Article

Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294

Insert

AtU6.29c14 promoter

Use

CRISPR

Expression

Plant

Mutation

T-to-C (-1 from TSS)

Promoter

AtU6.29c14 promoter

Available Since

Oct. 30, 2025

Availability

Academic Institutions and Nonprofits only

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p305N-6

Plasmid

#246289

Purpose

Evaluation of AtU6.1m3 promoter (Pol III promoter) sequence variation for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter line

Depositor

Chung-Jui Tsai

Article

Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294

Insert

AtU6.1m3 promoter

Use

CRISPR

Expression

Plant

Mutation

G-to-T (-57 from TSS) within USE

Promoter

AtU6.1m3 promoter

Available Since

Oct. 30, 2025

Availability

Academic Institutions and Nonprofits only

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p305N-15 (nonfunctional)

Plasmid

#246298

Purpose

Evaluation of HbU6.2 promoter (nonfunctional) (Pol III promoter) for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter line

Depositor

Chung-Jui Tsai

Article

Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294

Insert

HbU6.2 promoter (nonfunctional)

Use

CRISPR

Expression

Plant

Promoter

HbU6.2 promoter (nonfunctional)

Available Since

Oct. 30, 2025

Availability

Academic Institutions and Nonprofits only

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p305N-28

Plasmid

#246311

Purpose

Evaluation of PtU6.1c1 promoter (Pol III promoter) sequence variation for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter line

Depositor

Chung-Jui Tsai

Article

Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294

Insert

PtU6.1c1 promoter

Use

CRISPR

Expression

Plant

Mutation

C-to-A (-42 from TSS), A-to-C (-41)

Promoter

PtU6.1c1 promoter

Available Since

Oct. 30, 2025

Availability

Academic Institutions and Nonprofits only

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p305N-22

Plasmid

#246305

Purpose

Evaluation of MtU6.6-70 promoter (Pol III promoter) deletion (70 bp) for CRISPR-Cas9 editing of mEGFP in Nicotiana benthamiana and poplar reporter lines

Depositor

Chung-Jui Tsai

Article

Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294

Insert

MtU6.6-70 promoter

Use

CRISPR

Expression

Plant

Promoter

MtU6.6-70 promoter

Available Since

Oct. 30, 2025

Availability

Academic Institutions and Nonprofits only

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p305N-21

Plasmid

#246304

Purpose

Evaluation of MtU6.6-104 promoter (Pol III promoter) deletion (104 bp) for CRISPR-Cas9 editing of mEGFP in Nicotiana benthamiana and poplar reporter lines

Depositor

Chung-Jui Tsai

Article

Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294

Insert

MtU6.6-104 promoter

Use

CRISPR

Expression

Plant

Promoter

MtU6.6-104 promoter

Available Since

Oct. 30, 2025

Availability

Academic Institutions and Nonprofits only

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p305N-14

Plasmid

#246297

Purpose

Evaluation of CiU6.6c16 promoter (Pol III promoter) sequence variation for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter line

Depositor

Chung-Jui Tsai

Article

Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294

Insert

CiU6.6c16 promoter

Use

CRISPR

Expression

Plant

Mutation

T-to-C (-2 from TSS)

Promoter

CiU6.6c16 promoter

Available Since

Oct. 30, 2025

Availability

Academic Institutions and Nonprofits only

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p305N-11

Plasmid

#246294

Purpose

Evaluation of CiU6.3c8 promoter (Pol III promoter) sequence variation for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter line

Depositor

Chung-Jui Tsai

Article

Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294

Insert

CiU6.3c8 promoter

Use

CRISPR

Expression

Plant

Mutation

A-to-C (-23 from TSS)

Promoter

CiU6.3c8 promoter

Available Since

Oct. 30, 2025

Availability

Academic Institutions and Nonprofits only

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p305N-4 (nonfunctional)

Plasmid

#246287

Purpose

Evaluation of AtU6.1c1 promoter (nonfunctional) (Pol III promoter) sequence variation for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter line

Depositor

Chung-Jui Tsai

Article

Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294

Insert

AtU6.1c1 promoter (nonfunctional)

Use

CRISPR

Expression

Plant

Mutation

G-to-C at -15 from TSS

Promoter

AtU6.1c1 promoter (nonfunctional)

Available Since

Oct. 30, 2025

Availability

Academic Institutions and Nonprofits only

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p305N-20

Plasmid

#246303

Purpose

Evaluation of MtU6.6-189 promoter (Pol III promoter) deletion (189 bp) for CRISPR-Cas9 editing of mEGFP in Nicotiana benthamiana and poplar reporter lines

Depositor

Chung-Jui Tsai

Article

Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294

Insert

MtU6.6-189 promoter

Use

CRISPR

Expression

Plant

Promoter

MtU6.6-189 promoter

Available Since

Oct. 30, 2025

Availability

Academic Institutions and Nonprofits only

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p305N-16

Plasmid

#246299

Purpose

Evaluation of HbU6.2m1 promoter (Pol III promoter) sequence variation for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter line

Depositor

Chung-Jui Tsai

Article

Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294

Insert

HbU6.2m1 promoter

Use

CRISPR

Expression

Plant

Mutation

A-to-G (-56 from TSS), C-to-T (-29), G-to-A (-24)

Promoter

HbU6.2m1 promoter

Available Since

Oct. 30, 2025

Availability

Academic Institutions and Nonprofits only

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