We narrowed to 59,249 results for: {{{s}}}

1. Integrin beta5-2XEGFP

   Plasmid

   #139779

   Purpose

   Detecting Integrin beta5 by fluorescence microscopy

   Depositor

   Staffan Stromblad

   Article

   Lock et al Nat Cell Biol. 2018 Nov;20(11):1290-1302

   Insert

   Integrin subunit beta5 (ITGB5 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLV hU6-gRNA hUbC-VP64-dSaCas9-VP64-T2A-Thy1.1

   Plasmid

   #194279

   Purpose

   Expresses gRNA and VP64-dSaCas9-VP64 from lentiviral vector

   Depositor

   Charles Gersbach

   Article

   McCutcheon et al bioRxiv. 2023 May 1:2023.05.01.538906. d

   Inserts

   humanized VP64 dSaCas9 VP64 T2A Thy1.1
   gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hU6 and hUbC

   Available Since

   Feb. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Tol2-CAG::Mitbow

   Plasmid

   #158994

   Purpose

   Multicolor mitochondrial-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing mito-tethered mEYFP, mCherry or mCerulean upon Cre recombination)

   Depositor

   Jean Livet

   Article

   Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10

   Insert

   Mitbow
   

   Use

   Tags

   The default expressed EBFP2 is fused to H2B; oth…

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPBbsr2-rtTA2-TRE-SspB-mScarlet-I-MYPT169

   Plasmid

   #178528

   Purpose

   Expresses rtTA and SspB-mScarlet-I-MYPT (1-169 aa) in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

   Insert

   rtTA, SspB-mScarlet-I-MYPT (1-169aa)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG, Tet responsive element

   Available Since

   Dec. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-M13-TevC-IRES-SP6-Cre

   Plasmid

   #171620

   Purpose

   M13-TevC with Cre

   Depositor

   Hyungbae Kwon

   Article

   Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

   Insert

   M13-TevC-IRES-SP6-Cre
   

   Use

   AAV and Synthetic Biology

   Tags

   6x His

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available Since

   Oct. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pNICKclos1.0

   Plasmid

   #73639

   Purpose

   Genome editing for gene pyrE (CAC-002) in Clostridium acetobutylicum ATCC 824

   Depositor

   Sheng Yang

   Article

   Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

   Inserts

   Cas9 nickase
   sGRNA to pyrE
   

   Use

   E.coli-clostridium shuttle vector

   Tags

   Expression

   Mutation

   D10A

   Promoter

   j23119 and ptb

   Available Since

   June 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. FR_HNF4A8

   Plasmid

   #31114

   Depositor

   Gerhart Ryffel

   Article

   Erdmann et al Biol Chem. 2007 Jan . 388(1):91-106.

   Insert

   HNF4A (HNF4A Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   splice variant 8

   Promoter

   Available Since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AAVS1-Puro-CAG-fl-STOP-fl-ZEB2-GFP-Flag-WPRE-poly(A)

   Plasmid

   #165456

   Purpose

   Inducible ZEB2 expression from AAVS1 locus

   Depositor

   Madeline Lancaster

   Article

   Benito-Kwiecinski et al Cell. 2021 Apr 15;184(8):2084-2102.e19.

   Insert

   ZEB2 (ZEB2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p2T-cmv-SpCas9-TadCBEd-V106W-BlastR

   Plasmid

   #193849

   Purpose

   Express TadCBEd-V106W in mammalian cells with BlastR selection, Tol2 integration

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadCBEd-V106W
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-hSyn-DIO-Gluc-RMA-IRES-EGFP

    Plasmid

    #189630

    Purpose

    Expresses Gluc-RMA and EGFP in the presence of Cre recombinase. Double-floxed Gluc-RMA and EGFP for monitoring Cre-expressing neuronal populations.

    Depositor

    Jerzy Szablowski

    Article

    Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Inserts

    Gaussia luciferase fused to Fc
    IRES-EGFP
    

    Use

    AAV, Cre/Lox, and Luciferase

    Tags

    Gluc and IgG1 Fc

    Expression

    Mammalian

    Mutation

    Promoter

    IRES and hSyn

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA5-F-AF9

    Plasmid

    #49428

    Purpose

    Mammalian expression of Flag-tagged human AF9

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

    Insert

    AF-9 (MLLT3 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEXPqcxip-hFTSJ3-FLAG

    Plasmid

    #165463

    Purpose

    Human FTSJ3 retroviral mammalian expression vector. FLAG/Gateway modified pQCXIP vector with C-terminal FLAG epitope and bicistronic puromycin expression.

    Depositor

    Martin Dorf

    Article

    Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

    Insert

    FtsJ RNA 2'-O-methyltransferase 3 (FTSJ3 Human)
    

    Use

    Retroviral; Gateway

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/MSV, 5'LTR

    Available Since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLKO-puro-tetON-shRFP

    Plasmid

    #110940

    Purpose

    Tet-inducible shRNA targeting RFP

    Depositor

    William Hahn

    Article

    Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

    Insert

    shRFP
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    H1/TO

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pQFlag-USP7 CS puroR

    Plasmid

    #46752

    Purpose

    Retroviral vector that expresses catalytically inactive form of Flag-tagged human USP7

    Depositor

    Gordon Peters, Goedele Maertens

    Article

    Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

    Insert

    USP7 (USP7 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    C223S--catalytically inactive

    Promoter

    CMV

    Available Since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCas9-HE-Geminin

    Plasmid

    #109402

    Purpose

    Expresses Cas9 fusion to HE (HDR-enhancer) domain of human CtIP protein and Geminin degron

    Depositor

    Jean-Paul Concordet

    Article

    Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

    Insert

    HE-Geminin
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. EGFP

    Plasmid

    #232754

    Purpose

    Expresses EGFP

    Depositor

    Isaac Hilton

    Article

    Li et al Nucleic Acids Res. 2025 Feb 8;53(4):gkaf

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX SH3(2)-3R

    Plasmid

    #112091

    Purpose

    Bacterial expression plasmid containing a GST tag with 3 repeats of the second SH3 domain from human NCK1.

    Depositor

    Michael Rosen

    Article

    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

    Insert

    SH3(2)-3R (NCK1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Lac

    Available Since

    Feb. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFUW-TetO-Junb

    Plasmid

    #178441

    Purpose

    doxycycline-inducible expression of Mouse Junb in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

    Insert

    Junb (Junb Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAV106-pTDH3-mCherry-10xSumo-6xSIM

    Plasmid

    #116928

    Purpose

    Yeast integrative vector for expression of the SUMO-SIM complex

    Depositor

    Liam Holt

    Article

    Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

    Insert

    10xSUMO-6xSIM
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pp-mCAT1

    Plasmid

    #83948

    Purpose

    This plasmid co-expressed with VSVG allows the production of mCAT-1 gesicles rendering target cells permissive to transduction with Ecotropic lenti/retrovectors

    Depositor

    Philippe Mangeot, Vincent Lotteau

    Article

    Mangeot et al Mol Ther. 2011 Sep;19(9):1656-66. doi: 1

    Insert

    mCAT1 (Slc7a1 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge