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pTwist-SARS-CoV-2 Spike-deltaC18 JN.1.11.1

Plasmid

#223272

Purpose

Encodes SARS-CoV-2 spike JN.1.11.1 for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike JN.1.11.1 (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pLXC2B-PLAG1-3xFLAG

Plasmid

#228057

Purpose

lentiviral expression of human PLAG1 with 3xFLAG tag

Depositor

James Amatruda, Kenneth Chen

Article

Chen et al Genes Dev. 2018 Aug 1;32(15-16):996-1007

Insert

PLAG1-3xFLAG (PLAG1 Human)

Use

Lentiviral

Tags

3XFLAG

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCIG-FGFRdn

Plasmid

#80431

Purpose

Expression of a dominant-negative FGFR1 with a nuclear EGFP reporter in chick embryos

Depositor

Aixa Morales

Article

Martinez-Morales et al J Cell Biol. 2011 Aug 8;194(3):489-503.

Insert

Fibroblast growth factor receptor 1 (FGFR1 Chicken)

Tags

EGFP

Expression

Mammalian

Mutation

contains aa 1–425

Promoter

beta-actin promoter

Available Since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only
Psicheck Cdk2ap1Exon1

Plasmid

#178028

Purpose

expression vector to test translation efficiency by luciferase reporter.

Depositor

Lin He

Article

Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

Insert

Cdk2ap1 (Cdk2ap1 Mouse)

Use

Luciferase; Nonviral

Tags

Luciferase

Expression

Mammalian

Promoter

Cloned in

Available Since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

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ACTB-(A)1x

Plasmid

#112055

Purpose

ACTB mRNA tagged with 1 copy of Riboglow (variant A)

Depositor

Amy Palmer

Article

Braselmann et al Nat Chem Biol. 2018 Jul 30. pii: 10.1038

Insert

ACTB (ACTB Human)

Tags

Riboglow RNA tag

Expression

Mammalian

Mutation

1 copy of Riboglow tag (variant A) after stop cod…

Promoter

CMV

Available Since

June 29, 2018

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNeo/Cre

Plasmid

#67594

Purpose

Expresses an Neomycin-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Chiou et al Genes Dev. 2015 Jul 15;29(14):1576-85. d

Insert

sgNeo

Use

CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting

Expression

Mammalian

Promoter

U6

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC30A6

Plasmid

#132065

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC30A6 (SLC30A6 Human)

Expression

Mammalian

Available Since

Nov. 8, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-TOP3B

Plasmid

#172423

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

C-type lectin domain family 4 member D (CD antigen CD368) (CLEC4D Human)

Tags

MAC-tag

Expression

Mammalian

Promoter

CMV promoter

Available Since

Nov. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCKC775

Plasmid

#226626

Purpose

piggyBAC 7xTCF-LEF hCas9-T2A-A/T-biased2

Depositor

Chang Liu

Article

Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

Inserts

7xTCF-LEF, SpCas9, T2A, A/T-biased2 variant of TdT (DNTT S. pyogenes, Human)
EF1a promoter, sfGFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

7xTCF-LEF and EF1a

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCKC764

Plasmid

#226625

Purpose

piggyBAC pCAG-hCas9-T2A-Unbiased3

Depositor

Chang Liu

Article

Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

Inserts

CAG promoter, SpCas9, T2A, Unbiased3 variant of TdT (DNTT S. pyogenes, Human, Chicken)
EF1a promoter, blasticidin resistance

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV and EF1a

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pTRE-TAZ-CAMTA1

Plasmid

#235680

Purpose

Express TAZ-CAMTA1 fusion gene in mammalian cells using the doxycycline-inducible TRE promoter

Depositor

Duojia Pan

Article

Driskill et al Genes Dev. 2021 Apr 1;35(7-8):495-511. d

Insert

TAZ-CAMTA1 (WWTR1, CAMTA1 Human)

Tags

FLAG

Expression

Mammalian

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pQCXIP mScarlet-ER [TM(SAC1)]

Plasmid

#186572

Purpose

Expression of the Endoplasmic Reticulum marker mScarlet-ER [TM(SAC1)] in mammalian cells

Depositor

Catherine Tomasetto

Article

Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

SAC1 (SACM1L Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

C-terminal transmembrane region of the protein: 5…

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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TAPBPL pmRFP-N2 (721)

Plasmid

#62000

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

Insert

TAPBPL (TAPBPL Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
FRB-GFP-Gab2(Y604F/Y634F)

Plasmid

#188659

Purpose

FRB coupled to GFP tagged Gab2 with Shp2 binding sites mutated to Phe

Depositor

Andrei Karginov

Article

Fauser et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

Insert

GRB associated binding protein 2 (Gab2 Mouse)

Tags

FRB and GFP

Expression

Mammalian

Mutation

Y604F and Y634F

Promoter

CMV

Available Since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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LAMP2-bio-His

Plasmid

#51861

Purpose

Expresses full-length Lysosome-associated membrane glycoprotein 2 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

LAMP2 (LAMP2 Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pSUMO His6 SUMO SnRK2.6 (11-362)

Plasmid

#177847

Purpose

Bacterial Expression of SnRK2.6

Depositor

Karsten Melcher

Article

Ng et al Proc Natl Acad Sci U S A. 2011 Dec 27;10

Insert

SNF1-related protein kinase 2.6 (OST1 Mustard Weed)

Tags

6xHis-SUMO

Expression

Bacterial

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pRDA_122

Plasmid

#208095

Purpose

Perturb-Seq lentiviral gRNA expression vector with a 3' appended capture sequence to enable direct capture of CRISPR gRNAs for scRNA-seq

Depositor

John Doench

Article

Geiger-Schuller et al bioRxiv. 2023 Jan 24:2023.01.23.525198.

Insert

Puromycin resistance

Use

CRISPR and Lentiviral

Promoter

EF1a

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST40-mPTER

Plasmid

#224864

Purpose

Mammalian expression mouse PTER protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

mouse PTER (Pter Mouse)

Tags

C-term v5 and His tag

Expression

Mammalian

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET20b-mPTER

Plasmid

#222189

Purpose

Bacterial expression of mouse PTER protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

PTER (Pter Mouse)

Tags

6xHis and Strep

Expression

Bacterial

Available Since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLNCX2-OptoTGFBRs

Plasmid

#118965

Purpose

Retroviral expression OptoTGFBRs for transient or stable constitutive expression

Depositor

Won Do Heo

Article

Li et al ACS Synth Biol. 2018 Feb 16;7(2):443-451

Insert

OptoTGFBRs (Tgfb1, Tgfbr2 Mustard Weed, Mouse)

Use

Retroviral

Tags

tdTomato

Promoter

CMV

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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