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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,495 results for: pcdna

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Showing: 11301 - 11320 of 12495 results

  1. Rlucrarr3-Flash2

    Plasmid
    #74130
    Purpose
    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 139 of Arrestin3.
    Depositor
    Louis Luttrell
    Article
    Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi
    Insert
    Arrestin3
    Tags
    Renilla luciferase (rLuc)
    Expression
    Mammalian
    Mutation
    Insertion of FlAsH motif (CCPGCC) following amino…
    Available Since
    April 6, 2016
    Availability
    Academic Institutions and Nonprofits only

  2. CoV2-N-B.1.1.7

    Plasmid
    #177958
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (D3L, R203K, G204R, S235F). Used for generating RNA packaging virus-like particles.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Nucleocapsid (D3L, R203K, G204R, S235F) (N )
    Expression
    Mammalian
    Mutation
    D3L,R203K,G204R,S235F
    Available Since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177958-plasmid-map-sequence-id-376958

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  9. nuc-GafD-mTurboID-HA

    Plasmid
    #184641
    Purpose
    Expresses 3xHA-tagged GlycoID construct: GafD_short-miniTurboID in mammalian nucleus for O-GlcNAc interactome tagging
    Depositor
    Charlie Fehl
    Article
    Liu et al ACS Chem Biol. 2022 Jul 12. doi: 10.1021
    Insert
    nuc-GlycoID-HA
    Tags
    3xHA tag and Nuclear localization sequence
    Expression
    Mammalian
    Mutation
    GafD -> expressed 22-178 ; BirA* -> express…
    Promoter
    CMV
    Available Since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    184641-plasmid-map-sequence-id-366747

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  10. LUC05 (L/Cys/P)

    Plasmid
    #112146
    Purpose
    Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity
    Depositor
    Paul Copeland
    Article
    Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.
    Insert
    Luciferase coding region with wild type Cysteine 258 and wild-type PHGPx SECIS in the 3' UTR (Gpx4 Photinus pyralis, Rat)
    Expression
    Mammalian
    Mutation
    none
    Available Since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112146-plasmid-map-sequence-id-214888

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  17. ss-SNAP-TMD-NS3-Gal4

    Plasmid
    #112279
    Purpose
    Encodes the membrane-targeted TMD-NS3-Gal4, which exhibits drug-inducible transcription "turn-off" activity. SNAP-tag is fused to the extracellular region of the encoded protein construct.
    Depositor
    John Ngo
    Article
    Tague et al Nat Methods. 2018 Jul;15(7):519-522. doi
    Insert
    SNAP-TMD-NS3-Gal4
    Tags
    AU1 (N term on NS3) and HA (C term on NS3)
    Expression
    Mammalian
    Mutation
    NS3 mutant T54A
    Promoter
    CMV
    Available Since
    Sept. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    112279-plasmid-map-sequence-id-216922

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  20. CaMKIV-dCT

    Plasmid
    #126422
    Purpose
    Plasmid expresses constitutively active form of CaMK IV (C-terminal regulatory domains removed making the enzyme constitutively active)
    Depositor
    Douglas Black
    Article
    Xie et al Nature. 2001 Apr 19;410(6831):936-9. doi
    Insert
    Calcium/calmodulin-dependent protein kinase type IV (CAMK4 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Available Since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    126422-plasmid-map-sequence-id-249664

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Showing: 11301 - 11320 of 12495 results