We narrowed to 16,198 results for: grna

1. pKE010

   Plasmid

   #207910

   Purpose

   Level 0 for gRNA in C2 module (5' AGCC-3' TTCG)

   Depositor

   Alexis Maizel

   Article

   Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

   Insert

   pU3-AarI-scaffold-term
   

   Use

   CRISPR

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS019

   Plasmid

   #207913

   Purpose

   Level 0 for gRNA in D1 module (5' TCAG-3' TGAC)

   Depositor

   Alexis Maizel

   Article

   Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

   Insert

   pU6-AarI-scaffold-term
   

   Use

   CRISPR

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBAD-P19-EGFP

   Plasmid

   #196609

   Purpose

   siRNA vector expressing TMV P19 and a 720-long EGFP sequence as inverted repeat

   Depositor

   Franco Lucchini

   Article

   Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

   Insert

   p19 + EGFP inverted repeat sequence
   

   Tags

   6x His tag

   Expression

   Bacterial

   Promoter

   AraC

   Available Since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. lenti-Cas9-sgHPRT1

   Plasmid

   #196713

   Purpose

   CRISPR-KO. WT-SpCas9 and sgRNA targeting HPRT1. Editing-competent cells can be selected with 6-TG

   Depositor

   Bernhard Schmierer

   Article

   Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

   Insert

   Cas9-T2A-BSD-U6-sgHPRT1
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Promoter

   EF1a/hU6

   Available Since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV2ss-U6-sgGFP

   Plasmid

   #190899

   Purpose

   AAV vector expressing sgGFP

   Depositor

   Nicole Déglon

   Article

   Duarte et al Mol Ther Methods Clin Dev. 2023 Mar 27;2

   Insert

   sgRNA
   

   Use

   AAV and CRISPR

   Promoter

   U6

   Available Since

   March 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. p8271 LentiCRISPR v2 hygro sgSIGIRR-4

   Plasmid

   #193980

   Purpose

   Expression of spCas9 and sgRNA targeting SIGIRR

   Depositor

   Elizabeth White

   Article

   Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

   Insert

   spCas9 and sgRNA targeting SIGIRR (SIGIRR Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. GB1839

   Plasmid

   #193103

   Purpose

   GB-cassette for the expression of guide RNA targeting the DFR promoter in -376 position (gRNA2), with 2.1 Ms2 aptamer in the 3' of the scaffold.

   Depositor

   Diego Orzaez

   Article

   Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

   Insert

   GB_SynP gRNA2
   

   Use

   Synthetic Biology

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCK411.RR1

   Plasmid

   #192644

   Purpose

   Expresses sgRNA RR1 (target mRFP CDS) on ColE1-AmpR

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

   Insert

   BBa_J23119-RR1
   

   Use

   CRISPR and Synthetic Biology

   Promoter

   BBa_J23119

   Available Since

   Nov. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pX330A-Prdm14-L5-#1

   Plasmid

   #171513

   Purpose

   deletion of a genomic locus in Prdm14 gene

   Depositor

   Hans Schöler

   Article

   Aramaki et al EMBO Rep. 2021 Aug 4;22(8):e52553. doi:

   Insert

   Prdm14 (Prdm14 Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pX330A-Prdm14-L5-#2

    Plasmid

    #171514

    Purpose

    deletion of a genomic locus in Prdm14 gene

    Depositor

    Hans Schöler

    Article

    Aramaki et al EMBO Rep. 2021 Aug 4;22(8):e52553. doi:

    Insert

    Prdm14 (Prdm14 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pX330A-Prdm14-ex1-#1

    Plasmid

    #171507

    Purpose

    deletion of a genomic locus in Prdm14 gene

    Depositor

    Hans Schöler

    Article

    Aramaki et al EMBO Rep. 2021 Aug 4;22(8):e52553. doi:

    Insert

    Prdm14 (Prdm14 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pX330A-Prdm14-ex1-#2

    Plasmid

    #171508

    Purpose

    deletion of a genomic locus in Prdm14 gene

    Depositor

    Hans Schöler

    Article

    Aramaki et al EMBO Rep. 2021 Aug 4;22(8):e52553. doi:

    Insert

    Prdm14 (Prdm14 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pX330A-Prdm14-L3-#1

    Plasmid

    #171509

    Purpose

    deletion of a genomic locus in Prdm14 gene

    Depositor

    Hans Schöler

    Article

    Aramaki et al EMBO Rep. 2021 Aug 4;22(8):e52553. doi:

    Insert

    Prdm14 (Prdm14 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pX330A-Prdm14-L3-#2

    Plasmid

    #171510

    Purpose

    deletion of a genomic locus in Prdm14 gene

    Depositor

    Hans Schöler

    Article

    Aramaki et al EMBO Rep. 2021 Aug 4;22(8):e52553. doi:

    Insert

    Prdm14 (Prdm14 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pX330A-Prdm14-L4-#1

    Plasmid

    #171511

    Purpose

    deletion of a genomic locus in Prdm14 gene

    Depositor

    Hans Schöler

    Article

    Aramaki et al EMBO Rep. 2021 Aug 4;22(8):e52553. doi:

    Insert

    Prdm14 (Prdm14 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pX330A-Prdm14-L4-#2

    Plasmid

    #171512

    Purpose

    deletion of a genomic locus in Prdm14 gene

    Depositor

    Hans Schöler

    Article

    Aramaki et al EMBO Rep. 2021 Aug 4;22(8):e52553. doi:

    Insert

    Prdm14 (Prdm14 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPBO.ACT004

    Plasmid

    #182714

    Purpose

    Constituitvely expressed CasRx crRNA cloning vector, extended 3' terminator region

    Depositor

    Joseph Schoeniger

    Article

    Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

    Insert

    CasRx crRNA cloning backbone
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    Catalytically deactivated R295A, H300A, R849A, H8…

    Promoter

    pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)

    Available Since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX459-sg-tdTomato166/892

    Plasmid

    #179919

    Purpose

    Double cutting Cas9 plasmid with puromycin resistance and a single guide RNA targeting tdTomato fluorescent protein sites 166 and 892.

    Depositor

    Johan Sosa, Indeever Madireddy

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    tdTomato sgRNA
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PX552-mScn8a-3xHA-Syn-smFP-flag

    Plasmid

    #182563

    Purpose

    For mouse Nav1.6 knockin with 3xHA at the C-terminal

    Depositor

    James Zhe Liu

    Article

    Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

    Inserts

    mouse Scn8a gRNA and 3xHA donor DNA
    smFP-flag
    

    Use

    AAV and CRISPR

    Promoter

    EF1 and U6

    Available Since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PX552-mScn8a-HaloTag-V5-EF1-sfGFP

    Plasmid

    #182565

    Purpose

    For mouse Nav1.6 knockin with HaloTag-V5 at the C-terminal

    Depositor

    James Zhe Liu

    Article

    Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

    Inserts

    mouse Scn8a gRNA and HaloTag-V5 donor DNA
    sfGFP
    

    Use

    AAV and CRISPR

    Promoter

    EF1 and U6

    Available Since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge