Addgene

We narrowed to 2,388 results for: MEA;

Showing: 1121 - 1140 of 2388 results

Results per page:

Sort results by:

pcDNA3 AIF 1-90 mCherry FRB

Plasmid

#67530

Purpose

For expression of mCherry fused FRB to the mitochondrial innermembrane

Depositor

Stephen Tait

Article

Ichim et al Mol Cell. 2015 Mar 5;57(5):860-72. doi:

Inserts

AIF a.a. 1 - 90
mCherry
FRB

Use

Tags

AIF 1 - 90, FRB, and mCherry

Expression

Mammalian

Mutation

T->L

Promoter

CMV

Available since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pJT410 AAV-Rapamycin Inducible-NFZ-Citrine with bGH pA

Plasmid

#202059

Purpose

Inducible AAV activator

Depositor

Lacra Bintu, Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

AAV-Rapamycin Inducible-NFZ-Citrine with bGH pA

Use

AAV; Aav with reporter

Tags

Expression

Mammalian

Mutation

ITRs intact

Promoter

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR FGFR1-CD3ε-FusionRed

Plasmid

#188632

Purpose

Human FGFR1 labeled with CD3ε ITAMs for monitoring with CD3ε/ZtSH2 pYtag biosensor

Depositor

Jared Toettcher

Article

Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

Insert

FGFR1 (FGFR1 Synthetic, Human)

Use

Lentiviral

Tags

CD3ε-FusionRed

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX_hDDX5_FL_wt

Plasmid

#88871

Purpose

inducible expression of GST-DDX5 in bacteria

Depositor

Dan Littman

Article

Huang et al Nature. 2015 Dec 24;528(7583):517-22. do

Insert

DDX5 (DDX5 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Tac

Available since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 AIF-mClover

Plasmid

#129575

Purpose

Expression of mClover fused AIF

Depositor

Stephen Tait

Article

Riley et al EMBO J. 2018 Sep 3;37(17). pii: embj.201

Insert

AIF (AIFM1 Human)

Use

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLY099-RetroEFS-BCMABBz-PRODH2-WPRE

Plasmid

#192200

Purpose

Retro-BCMACAR-PRODH2

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

Retro-BCMACAR-PRODH2 (TNFRSF17 Synthetic)

Use

Retroviral; Mammalian expression

Tags

Expression

Mutation

NA

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMThy1.1_hDDX5_FL

Plasmid

#88873

Purpose

constitutive expression of DDX5 in mammalian cells

Depositor

Dan Littman

Article

Huang et al Nature. 2015 Dec 24;528(7583):517-22. do

Insert

DDX5 (DDX5 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

MSCV

Available since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMMK ENTR 4 CMV GST mTagBFP

Plasmid

#206259

Purpose

ENTR Vector 4 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mTagBFP localised to Golgi organelles under the control of CMV promoter.

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

GTS mTagBFP

Use

Multimate/gateway entr 4

Tags

BFGT1 (Golgi localisation peptide)

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX_hDDX5_FL_D248N

Plasmid

#88872

Purpose

inducible expression of GST-DDX5 D248N in bacteria

Depositor

Dan Littman

Article

Huang et al Nature. 2015 Dec 24;528(7583):517-22. do

Insert

DDX5 (DDX5 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

D248N

Promoter

Tac

Available since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAR010 Lenti pEF-dEnAsCas12a-NLS-3XHA-T2A-BSD

Plasmid

#195543

Purpose

dCas12a effector fusion for manipulating transcription

Depositor

Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

Lenti pEF-dEnAsCas12a-NLS-3XHA-T2A-BSD

Use

CRISPR and Lentiviral

Tags

3XHA

Expression

Mammalian

Mutation

Promoter

pEF1a

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAuroraA GFP-AURKA-GFP

Plasmid

#157765

Purpose

Expression of AURKA fused to GFP at both ends in mammalian cells under AurkA promoter

Depositor

Marc Tramier

Article

Bertolin et al ACS Sens. 2019 Aug 23;4(8):2018-2027. do

Insert

AURKA (AURKA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

promoter CMV replaced by AurkA promoter

Promoter

AURKA minimal

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
mCerulean3-Bcl-W-FUGW

Plasmid

#166751

Purpose

Express mCerulean3 fused to Bcl-2 family protein, Bcl-W, in a plasmid compatable for lentiviral infection. Used to make stable BMK-DKO cell lines.

Depositor

David Andrews

Article

Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

Insert

Bcl-W (Bcl2l2 Mouse)

Use

Lentiviral

Tags

mCerulean3

Expression

Mutation

Promoter

human ubiquitin C

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJT311 AAV-Rapamycin Inducible-NZF-Citrine

Plasmid

#202060

Purpose

Inducible AAV activator

Depositor

Lacra Bintu, Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

AAV-Rapamycin Inducible-NZF-Citrine

Use

AAV; Aav with reporter

Tags

Expression

Mammalian

Mutation

ITR deletion

Promoter

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
SITS-MmCav1-EGFP-APEX2

Plasmid

#170525

Purpose

Vector expression of MmCav1-EGFP-APEX2 in Leishmania cell free system. Parton lab clone JEJ

Depositor

Rob Parton

Article

Rae et al Elife. 2021 Apr 27;10. pii: 64630. doi:

Insert

Caveolin1 (Cav1 Mouse)

Use

Synthetic Biology

Tags

APEX2

Expression

Mutation

Promoter

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 AIF-mScarlet

Plasmid

#129576

Purpose

Expression of mScarlet fused AIF

Depositor

Stephen Tait

Article

Riley et al EMBO J. 2018 Sep 3;37(17). pii: embj.201

Insert

AIF (AIFM1 Human)

Use

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJT216 Lenti pEF-dCas9-3XFLAG-LibCloneSite-T2A-tagBFP-P2A-Blast

Plasmid

#187320

Purpose

dCas9 expression vector with cloning site for C terminal fusions

Depositor

Lacra Bintu, Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Mutation

Promoter

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBGE3.0

Plasmid

#165988

Purpose

Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymB origin and Nm/Kan resistance.

Depositor

Bogumil Karas

Article

Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do

Inserts

RK2/RP4 origin of transfer
repA1B1C1
nourseothricin N-acetyl transferase
neomycin resistance

Use

Synthetic Biology; Bacterial and yeast cloning; c…

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pNL3.1-Erg

Plasmid

#182706

Purpose

Express Erg enhancer in mammalian cells

Depositor

Robert Rowe

Article

Wang et al Cell Rep. 2022 Apr 5;39(1):110587. doi:

Insert

Enhancer of Erythroblast transformation-specific-Related Gene (Erg Mouse)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Myc-Nix-delta TM

Plasmid

#100755

Purpose

Mammalian expression of Nix dominant negative

Depositor

Joseph Gordon

Article

Mughal et al Cell Death Differ. 2018 Mar 6. pii: 10.1

Insert

Nix-_TM (BNIP3L Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

TM deletion

Promoter

CMV

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Venus-Bik-4E-pEGFP-C1

Plasmid

#166744

Purpose

Express Venus fused to the N-terminus of the Bcl-2 family protein, Bik with mutation in BH3 domain to disrupt binding anti-apoptotic proteins

Depositor

David Andrews

Article

Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

Insert

Bik-4E (BIK Human)

Use

Tags

Venus

Expression

Mammalian

Mutation

4 hydrophobic residues in the BH3 region mutated …

Promoter

CMV

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge