Addgene

We narrowed to 2,432 results for: gem

Showing: 1121 - 1140 of 2432 results

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pACYCDuet-1:ChrA-C63S

Plasmid

#225080

Purpose

Expresses precursor peptide ChrA-C63S in E.coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

Insert

ChrA-C63S

Use

Tags

His6

Expression

Bacterial

Mutation

C63S

Promoter

T7

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCDFDuet-1:ChrH-ChrI

Plasmid

#225083

Purpose

Expresses multinuclear non-heme iron dependent oxidative enzyme ChrH and partner protein ChrI in E.coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

Insert

ChrH

Use

Tags

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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TBPL1 KO 1

Plasmid

#200210

Purpose

gRNA for TBPL1 KO

Depositor

Sheila Teves

Article

Kwan et al Elife. 2023 Mar 30;12:e83810. doi: 10.75

Insert

TBP1 KO 1 (Tbpl1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

None

Promoter

U6

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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TBPL1 KO 2

Plasmid

#200211

Purpose

gRNA for TBPL1 KO

Depositor

Sheila Teves

Article

Kwan et al Elife. 2023 Mar 30;12:e83810. doi: 10.75

Insert

TBP1 KO 2 (Tbpl1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

None

Promoter

U6

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pGFP-Ub-VPS13D

Plasmid

#176462

Purpose

expressing GFP-Ub marked VPS13D in mammalian cells. GFP-Ub will be cleaved by DUBs to express untagged VPS13D

Depositor

Richard Youle

Article

Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

VPS13D (VPS13D Human)

Use

Tags

GFP-Ub

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-Ub-VPS13D N3521S

Plasmid

#176463

Purpose

expressing VPS13D patient mutant N3521S in mammalian cells

Depositor

Richard Youle

Article

Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

VPS13D (VPS13D Human)

Use

Tags

GFP-Ub

Expression

Mammalian

Mutation

N3521S mutation

Promoter

CMV

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pTH32 [pmyo-3::YFP-CaCyclOp(A-2x)::SL2::mCherry]

Plasmid

#168168

Purpose

Expression of YFP-CaCyclOp(A-2x) in BWMs of C. elegans

Depositor

Alexander Gottschalk

Article

Henss et al Br J Pharmacol. 2021 Mar 18. doi: 10.111

Insert

YFP-CaCyclOp(A-2x)

Use

Tags

YFP

Expression

Worm

Mutation

E497K and C566D

Promoter

Available since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pTH42 [punc-17::YFP-CaCyclOp(A-2x)::SL2::mCherry]

Plasmid

#168171

Purpose

Expression of YFP-CaCyclOp(A-2x) in cholinergic motor neurons of C. elegans

Depositor

Alexander Gottschalk

Article

Henss et al Br J Pharmacol. 2021 Mar 18. doi: 10.111

Insert

YFP-CaCyclOp(A-2x)

Use

Tags

YFP

Expression

Worm

Mutation

Promoter

Available since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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Fuw CBX8 quad

Plasmid

#121118

Purpose

conditional expression

Depositor

Emily Dykhuizen

Article

Connelly et al Nucleic Acids Res. 2018 Dec 29. pii: 526

Insert

CBX8 (R19A,R20A, R22A, W32A) (CBX8 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

R19A, R20A, R22A, W32A

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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rs210-major

Plasmid

#113377

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs2104047 major allele (A)

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs2104047 major allele

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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rs210-minor

Plasmid

#113378

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs2104047 minor allele (G)

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs2104047 minor allele

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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rs438-major

Plasmid

#113369

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs4384382 major allele (A)

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs4384382 major allele

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only
rs344-minor

Plasmid

#113366

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs34466261 minor allele (A)

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs34466261 minor allele

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only

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rs438-minor

Plasmid

#113370

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs4384382 minor allele (G)

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs4384382 minor allele

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
rs294-minor

Plasmid

#113374

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs2941742 minor allele (G)

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs2941742 minor allele

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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rs294-major

Plasmid

#113373

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs2941742 major allele (A)

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs2941742 major allele

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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rs281-minor

Plasmid

#113372

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs281038 minor allele (G)

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs281038 minor allele

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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rs281-major

Plasmid

#113371

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs281038 major allele (A)

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs281038 major allele

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
rs117-major

Plasmid

#113367

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs11716984 major allele (A)

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs11716984 major allele

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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rs117-minor

Plasmid

#113368

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs11716984 minor allele (T)

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs11716984 minor allele

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge