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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,440 results for: pir

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Showing: 1121 - 1140 of 4440 results

  1. pGBW-m4046938

    Plasmid
    #145599
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145599-plasmid-map-sequence-id-300265

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  2. Spike Display_B.1.1.7

    Plasmid
    #172735
    Purpose
    Expression vector for SARS-CoV-2 (B.1.1.7) spike used in the Spike Display system
    Depositor
    IIya Finkelstein
    Article
    Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e
    Insert
    SARS-CoV-2 S B.1.1.7 (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…
    Expression
    Mammalian
    Mutation
    Ectodomain only (AAs 1-1208); 682-685 (furin site…
    Promoter
    CMV
    Available Since
    Aug. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    172735-plasmid-map-sequence-id-340032

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  7. pαH-S-GSAS-OMICRON.BA.2

    Plasmid
    #184829
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON.BA.2 with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987
    Depositor
    Priyamvada Acharya
    Article
    Stalls et al Cell Rep. 2022 Jun 28;39(13):111009. doi
    Insert
    S-GSAS-OMICRON.BA.2 (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available Since
    June 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    184829-plasmid-map-sequence-id-363836

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  12. pQZ210

    Plasmid
    #106414
    Purpose
    Co-expresses trans SNARE complex with pDC183 in E.coli
    Depositor
    Axel Brunger
    Article
    Zhou et al Nature. 2017 Aug 24;548(7668):420-425. d
    Inserts
    Rat Synaptobrevin-2 fragment 28-66 (Vamp2 Synthetic)
    Syntaxin-1A fragment 191-256 (Stx1a Synthetic, Rat)
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Fragment 191-256 , Codon optimization and Fragmen…
    Promoter
    T7
    Available Since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    106414-plasmid-map-sequence-id-204167

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  16. EGFP-p300 (D1399Y)

    Plasmid
    #191763
    Purpose
    Expression of EGFP fused to catalytically dead core p300 histone acetyltransferase (D1399Y)
    Depositor
    Daniel Gerlich
    Article
    Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:
    Insert
    FRB-EGFP-p300 core (D1399Y) (EP300 Human)
    Tags
    EGFP (N-terminal of p300 core (D1399Y)) and FRB (…
    Expression
    Bacterial and Mammalian
    Mutation
    catalytic D1399Y mutation in p300
    Promoter
    EF1alpha
    Available Since
    Feb. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    191763-plasmid-map-sequence-id-383798

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  20. pCRI001-pGEM-LS-Bar-PgpdA-dLbCas12a-VPR-Ttrpc-LS

    Plasmid
    #140193
    Purpose
    Chromosomal integration of PgpdA-dLbCas12a-VPR-TtrpC. Contains 1kb homology arms for Aspergillus nidulans and the bar gene for glufosinate selection. Filamentous fungi integrative vector.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Inserts
    dLbCas12a-VPR
    Bar
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2736011,2737053)
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2790295,2791327)
    Use
    CRISPR and Synthetic Biology; Fungal genome integ…
    Tags
    VPR (VP64-p65-Rta), NLS
    Mutation
    D832A DNAse deactivated
    Promoter
    PgpdA and PtrpC
    Available Since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    140193-plasmid-map-sequence-id-309648

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Showing: 1121 - 1140 of 4440 results