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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 23,151 results for: REP

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Showing: 11421 - 11440 of 23151 results

  1. pZLSLD.R

    Plasmid
    #51511
    Purpose
    Single component LSL vector. Contains 2x35S:Zif268:FokI outside of the replicon borders. Within the replicon is the us:NPTII template.
    Depositor
    Daniel Voytas
    Article
    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:
    Inserts
    Zif268:FokI
    us:NPTII repair template
    Use
    Plant t-dna plasmid
    Tags
    NLS
    Promoter
    2x35S
    Available Since
    May 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    51511-plasmid-map-sequence-id-78678

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  5. pCR8/GW_TOPO_Cdk12_Nterm_FlagHa

    Plasmid
    #127177
    Purpose
    N-terminal Flag- HA- tandem epitope tagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into Gateway entry vector
    Depositor
    Phil Sharp
    Article
    Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:
    Insert
    N-terminally Flag- HA- Epitope Tagged Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
    Use
    Entry vector with transgene for gateway cloning
    Tags
    Flag- HA- Tandem Epitopes
    Promoter
    None
    Available Since
    July 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    127177-plasmid-map-sequence-id-246566

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  6. pTU2-A-RFP (colE1 origin)

    Plasmid
    #74093
    Purpose
    pTU2-A backbone, with RFP negative selection and colE1 (medium-copy) origin of replication
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Golden Gate destination vector - Level 2, 4 TU's with RFP and colE1 origin of replication
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    R0010 Const. Promoter controlling RFP
    Available Since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    74093-plasmid-map-sequence-id-138340

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  7. HeFSpCas9

    Plasmid
    #92355
    Purpose
    Expression plasmid for human codon-optimized increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity Cas9-NLS
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A
    Promoter
    Cbh
    Available Since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    92355-plasmid-map-sequence-id-197617

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  8. LRRC59-His-bio

    Plasmid
    #52330
    Purpose
    Expresses full-length Leucine rich repeat containing 59 ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    LRRC59 (LRRC59 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52330-plasmid-map-sequence-id-81047

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  9. pJN601

    Plasmid
    #59790
    Purpose
    zf1::yfp fusion with floxed unc-119(+) within an intron of yfp. For creating zf1::yfp knock-ins using unc-119 as a marker
    Depositor
    Jeremy Nance
    Article
    Armenti et al Development. 2014 Dec;141(23):4640-7. do
    Inserts
    ZF1 domain from pie-1
    N-terminal half of yfp with introns
    intron containing floxed unc-119 gene in reverse orientation
    C-terminal half of yfp with introns
    Expression
    Worm
    Available Since
    Oct. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    59790-plasmid-map-sequence-id-97553

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  10. pCVL TLR 3.1 (Sce target) Ef1a iRFP Puro

    Plasmid
    #50417
    Purpose
    Fluorescent reporter for DNA cleavage/repair in mammalian cells expressing iRFP with selectable puromycin marker.
    Depositor
    Andrew Scharenberg
    Inserts
    eGFP with I-Sce I TS
    +3 mCherry
    Use
    Lentiviral
    Tags
    T2A dislinker and iRFP
    Expression
    Mammalian
    Mutation
    embedded I-Sce I TS from 163-185
    Promoter
    SFFV
    Available Since
    Jan. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    50417-plasmid-map-sequence-id-75349

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  16. TRE3G-PE2-P2A-BFP

    Plasmid
    #231580
    Purpose
    Expresses the PE2 prime-editing machinery fused to BFP (PE2-P2A-BFP), under the control of a TRE3G promoter. This promoter is responsive to doxycycline bound to the rtTA protein
    Depositor
    Jesse Engreitz
    Article
    Martyn et al bioRxiv [Preprint]. 2023 Dec 21:2023.12.
    Insert
    PE2-P2A-BFP
    Use
    Lentiviral
    Tags
    P2A-BFP
    Expression
    Mammalian
    Promoter
    TRE3G
    Available Since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    231580-plasmid-map-sequence-id-462114

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  20. LRRC-bio-His

    Plasmid
    #53333
    Purpose
    Expresses full-length Leucine-rich repeat-containing protein 32 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    LRRC (LRRC32 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    53333-plasmid-map-sequence-id-83064

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Showing: 11421 - 11440 of 23151 results