168,010 results

1. pBS-L1PA1CH blast

   Plasmid

   #69612

   Purpose

   Expresses a blasticidin tagged codon optimized L1PA1

   Depositor

   Astrid Roy-Engel

   Insert

   L1PA1
   

   Tags

   blast cassette

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-Sport6-CD63-pHuji

   Plasmid

   #130902

   Purpose

   Expression of CD63-pHuji for visualization of multivesicular body-plasma membrane fusion.

   Depositor

   DM Pegtel

   Article

   Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

   Insert

   CD63-pHuji (CD63 synthetic, Human)
   

   Tags

   pHuji

   Expression

   Mammalian

   Mutation

   pHuji inserted between Gln-36 and Leu-37 in extra…

   Promoter

   CMV

   Available Since

   Oct. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pB025

   Plasmid

   #183092

   Purpose

   Expresses FnCas12a in Bacteroides and used for genome editing

   Depositor

   Lei Dai

   Article

   Zheng et al ACS Synth Biol. 2022 Jan 21;11(1):464-47

   Insert

   FnCas12a, gRNA, Promoter, HAB, TetR,
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   P1TDPGH023

   Available Since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV_BiPVe3_dTomato_nlsdTomato

   Plasmid

   #213940

   Purpose

   AAV construct expressing dTomato driven by PV+ basket cell-targeting enhancer. Alias: pAAV_WP0003_dTomato_nlsdTomato

   Depositor

   Gordon Fishell

   Article

   Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

   Has Service

   AAV PHP.eB and AAV1

   Insert

   dTomato_P2A_nls.dTomato
   

   Use

   AAV

   Available Since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHJL1

   Plasmid

   #199376

   Purpose

   Encodes dual cistronic cytosolic GCaMP6s and mScarlet-I optimized for C. elegans expression

   Depositor

   Hang Lu

   Insert

   GCaMP6s::SL2::mScarlet-I
   

   Expression

   Bacterial

   Mutation

   no mutations

   Available Since

   April 24, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pSynSRE-T-Luc

   Plasmid

   #60444

   Purpose

   Uses the hamster HMG-CoA synthase promoter sequence (-324/-225) and minimal HMG-CoA sythnase TATA box (-28/+39) to study SREBP-dependent transcriptional activation.

   Depositor

   Timothy Osborne

   Article

   Dooley et al J Biol Chem. 1998 Jan 16;273(3):1349-56.

   Insert

   HMG-CoA synthase promoter (-324/-225)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Promoter

   HMG-CoA synthase (-324/-225) & TATAA (-28/+39)

   Available Since

   Feb. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti_EF1a_Flag_TP53_WT

   Plasmid

   #183112

   Purpose

   Expresses flag-tagged p53 in mammalian cells

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   TP53 (TP53 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   See Depositor Comments Below

   Promoter

   EF1a

   Available Since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQE31-rsEGFP2

   Plasmid

   #102879

   Purpose

   Expresses rsEGFP2 in bacterial cells

   Depositor

   Stefan Jakobs

   Article

   Grotjohann et al eLife. 2012 Dec 31:1:e00248. doi: 10.755

   Insert

   rsEGFP2
   

   Tags

   6xHisTag

   Expression

   Bacterial

   Available Since

   Jan. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. LentiCRISPR v2 hBAK

   Plasmid

   #129579

   Purpose

   CRISPR deletion of human BAK

   Depositor

   Stephen Tait

   Article

   Lopez et al Nat Commun. 2016 Feb 2;7:10538. doi: 10.

   Insert

   gRNA vs human BAK (BAK1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. mRFP-Rab7

    Plasmid

    #14436

    Purpose

    Mammalian expression of Rab7 fused to mRFP. Used for localization to the late endosome, and other early endosomal vacuoles.

    Depositor

    Ari Helenius

    Article

    Vonderheit et al PLoS Biol. 2005 Jul . 3(7):e233.

    Insert

    Rab 7 (RAB7A Canis familialis)
    

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    3 silent mutations near the C-terminus

    Available Since

    March 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR-tdPP7-3xmCherry

    Plasmid

    #74926

    Purpose

    Expresses tdPP7-3xmCherry

    Depositor

    Marvin Tanenbaum

    Article

    Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

    Insert

    PP7-3xmCherry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. bdSUMO-HA2-GFP-HiBit

    Plasmid

    #171017

    Purpose

    For bacterial expression of bdSUMO tagged muGFP with HA2 peptide and NanoLuc complementation peptide variant #86

    Depositor

    Angus Johnston

    Article

    Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

    Insert

    14x-His-bdSUMO-HA2-muGFP-HiBiT
    

    Use

    Luciferase

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSLTS

    Plasmid

    #59386

    Purpose

    Expresses the lambda Red recombinase under control of the arabinose-inducible P(araB) promoter and I-SceI under control of the anhydrotetracycline-inducible P(tetA) promoter.

    Depositor

    Shelley Copley

    Article

    Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

    Type

    Empty backbone

    Use

    Genome engineering

    Expression

    Bacterial

    Available Since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. TFORF0794

    Plasmid

    #142239

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ATF7 (ATF7 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-EML4-ALK variant 3a I1171N/D1203N

    Plasmid

    #183833

    Purpose

    Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK I1171N/D1203N mutation in mammalian cells

    Depositor

    Aaron Hata

    Article

    Shiba-Ishii et al Nat Cancer. 2022 Jun 20. pii: 10.1038/s4

    Inserts

    ALK Receptor Tyrosine Kinase (ALK Human)
    EMAP Like 4 (EML4 Human)
    

    Use

    Lentiviral

    Tags

    The fusion protein of EML4 exon 1-6a and ALK exon…

    Expression

    Mammalian

    Mutation

    I1171N/D1203N

    Promoter

    CMV

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. ecAT3.10

    Plasmid

    #107215

    Purpose

    Mammalian expression plasmid to target the ecAT3.10 extracellular ATP sensor to the cell surface.

    Depositor

    Mathew Tantama

    Article

    Conley et al PLoS One. 2017 Nov 9;12(11):e0187481. do

    Insert

    ecAT3.10
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR221_SLC7A9

    Plasmid

    #132245

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC7A9 (SLC7A9 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 21, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pTXB1-AbaSFI

    Plasmid

    #86900

    Purpose

    Express CBD and intein tag containing AbaSI enzyme full length

    Depositor

    Matthias Bochtler

    Article

    Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

    Insert

    AbaSDFI
    

    Tags

    CBD

    Promoter

    T7

    Available Since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. FUW

    Plasmid

    #14882

    Depositor

    David Baltimore

    Article

    Lois et al Science. 2002 Feb 1. 295(5556):868-72.

    Insert

    flap-Ub promoter-WRE
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    May 25, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSH-EFIRES-P-AtAFB2-mCherry

    Plasmid

    #129716

    Purpose

    AAVS1 safe harbor targeting vector expressing AtAFB2-mCherry

    Depositor

    Elina Ikonen

    Article

    Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

    Insert

    AFB2 (AFB2 Mustard Weed)
    

    Use

    CRISPR and TALEN; Human safe harbor locus (a avs1…

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    EF1a

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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