166,542 results

1. pLenti6.2_3xFLAG_V5_ mmIfih1_V5

   Plasmid

   #160110

   Purpose

   Expresses murine Ifih1 in mammalian cells

   Depositor

   Rizwan Haq

   Article

   Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

   Insert

   Ifih1 (Ifih1 Mouse)
   

   Use

   Lentiviral

   Tags

   3xFLAG, V5 and V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEv-Fat4-citrine

   Plasmid

   #112854

   Purpose

   Constitutive expression of human fat4 protein fused to citrine fluorescent protein

   Depositor

   David Sprinzak

   Article

   Loza et al Elife. 2017 Aug 18;6. pii: 24820. doi: 1

   Insert

   FAT4 (FAT4 Human)
   

   Use

   Tags

   Citrine

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-GRAB-gDA3m (AAV1)

   Viral Prep

   #208698-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV-hSyn-GRAB-gDA3m (#208698). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB-gDA3m plasmid DNA. Syn-driven expression of the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3m in neurons. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Yulong Li

   Article

   Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Promoter

   Syn

   Tags

   None

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

4. pET21a-BirA

   Plasmid

   #20857

   Depositor

   Alice Ting

   Article

   Howarth et al Proc Natl Acad Sci U S A. 2005 May 24. 1

   Insert

   BirA
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. DTA in pCDEST2

   Plasmid

   #223705

   Purpose

   Mammalian expression vector encoding diptheria toxin A

   Depositor

   Rob Parton

   Article

   T. D. et al bioRxiv

   Insert

   Diphtheria toxin A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCherry-Cytb5

   Plasmid

   #182579

   Purpose

   Expresses mCherry targeted to the ER via the CytB5RR tail anchor sequence

   Depositor

   Uri Manor

   Article

   Schiavon et al Nat Methods. 2020 Sep;17(9):917-921. doi

   Insert

   mCherry-Cytb5
   

   Use

   Tags

   Cytb5

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC57-CmYLCV

   Plasmid

   #205245

   Purpose

   For plant prime editing in wheat plants or monocotyledons protoplasts

   Depositor

   Zong Yuan

   Article

   Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

   Insert

   CmYLCV-csy4_binding_site-esgRNA-tevopreQ1-csy4_binding_site
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CmYLCV

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-syn-FLP-FRT-iGluSnFR4f-NGR-WPRE

   Plasmid

   #234454

   Purpose

   AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

   Depositor

   GENIE Project

   Article

   Aggarwal et al bioRxiv 2025.03.20.643984

   Insert

   iGluSnFR4f-NGR
   

   Use

   AAV; Flp/frt

   Tags

   IgK chain and Myc epi tag-NGR GPI anchor

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40

   Plasmid

   #100853

   Purpose

   AAV expression of Cre recombinase-activated jRGECO1a, a red fluorescent calcium sensor protein, from the Synapsin promoter

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Has Service

   AAV Retrograde and AAV1

   Insert

   jRGECO1a
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available Since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTol1-UAS:CoChR-tdTomato

    Plasmid

    #124233

    Purpose

    Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin CoChR fused to the red fluorescent protein tdTomato

    Depositor

    Isaac Bianco

    Article

    Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

    Insert

    CoChR-tdTomato
    

    Use

    Zebrafish expression

    Tags

    tdTomato

    Expression

    Mutation

    Promoter

    14xUAS-E1b

    Available Since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV pEF1a-DIO-FLPo-WPRE-hGHpA

    Plasmid

    #87306

    Purpose

    An AAV vector expresses FLPo in a Cre recombinase-dependent manner.

    Depositor

    Li Zhang

    Article

    Zingg et al Neuron. 2017 Jan 4;93(1):33-47. doi: 10.

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    Cre-dependent mouse codon-optimized Flp recombinase.
    

    Use

    AAV

    Tags

    N/A

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. LifeAct-GFP-Fis1

    Plasmid

    #182576

    Purpose

    Expresses LifeAct-GFP targeted to the outer mitochondrial membrane via the Fis1 tail anchor sequence

    Depositor

    Uri Manor

    Article

    Schiavon et al Nat Methods. 2020 Sep;17(9):917-921. doi

    Insert

    LifeAct-GFP-Fis1
    

    Use

    Tags

    Fis1 and LifeAct

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pB-CMPE-ePPEplus

    Plasmid

    #205246

    Purpose

    For plant prime editing in wheat plants

    Depositor

    Zong Yuan

    Article

    Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

    Insert

    Csy4-c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Detailed in manuscript

    Promoter

    Available Since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. LMAN1-bio-His

    Plasmid

    #51922

    Purpose

    Expresses full-length ERGIC-53 protein precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    LMAN1 (LMAN1 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.3_SARS2_JN.1

    Plasmid

    #218678

    Purpose

    expressing SARS-CoV-2 JN.1 spike protein for pseudovirus production

    Depositor

    David Nemazee

    Insert

    Spike (JN.1)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L455S on the basis of BA.2.86

    Promoter

    CMV

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pExp-MBP

    Plasmid

    #112568

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and MBP fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8xHis-MBP and Strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1/cyto-iAspSnFR

    Plasmid

    #201394

    Purpose

    CMV overexpression of cyto-iAspSnFR in mammalian cell lines

    Depositor

    Kai Johnsson

    Article

    Hellweg et al Cell Chem Biol. 2024 May 20:S2451-9456(2

    Insert

    cyto-iAspSnFR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    gltI5-253: Y42F, S72A, T92D, G111T, K192R, Y211W,…

    Promoter

    CMV

    Available Since

    May 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA3-HA-Akt1

    Plasmid

    #73408

    Purpose

    Expresses human AKT1 with a N-terminus HA tag

    Depositor

    Jie Chen

    Article

    Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pExp-NusA

    Plasmid

    #112571

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and NusA fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8His and Strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-hSyn-GRAB-HaloDA1.0

    Plasmid

    #234408

    Purpose

    Expresses the chemigenetic dopamine (DA) sensor GRAB_HaloDA1.0 in neurons

    Depositor

    Yulong Li

    Article

    Zheng et al Science. 2025 Jun 5;388: 6751. doi: http

    Insert

    GPCR activation based chemigenetic dopamine (DA) sensor GRAB_HaloDA1.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available Since

    June 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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