163,774 results

1. Human GeCKO Lentiviral sgRNA Library v2 (LentiCRISPR)

   Pooled Library

   #1000000048

   Purpose

   CRISPR gRNA knockout pooled library for targeting the human genome

   Depositor

   Feng Zhang

   Available since

   May 8, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA3.1-GFP(1-10)

   Plasmid

   #70219

   Purpose

   Expresses GFP(1-10) in mammalian cells

   Depositor

   Bo Huang

   Article

   Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

   Has Service

   Cloning Grade DNA

   Insert

   GFP(1-10)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCasPA

   Plasmid

   #113347

   Purpose

   Bacterial expression of Cas9 nuclease and λ-Red system in Pseudomonas aeruginosa

   Depositor

   Quanjiang Ji

   Article

   Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNF02-mChartreuse

   Plasmid

   #219397

   Purpose

   Constitutive expression of mChartreuse in E. coli

   Depositor

   Christian Lesterlin

   Article

   Fraikin et al Sci Adv. 2025 Apr 18;11(16):eads6201. do

   Insert

   mChartreuse
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   proDp

   Available since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET His6 Sumo TEV LIC cloning vector (1S)

   Plasmid

   #29659

   Depositor

   Scott Gradia

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   6xHis, SUMO, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. EGFP-alphasynuclein-WT

   Plasmid

   #40822

   Depositor

   David Rubinsztein

   Article

   Furlong et al Biochem J. 2000 Mar 15;346 Pt 3:577-81.

   Insert

   SNCA (SNCA Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CaMKIIa-eNpHR 3.0-EYFP (AAV9)

   Viral Prep

   #26971-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV-CaMKIIa-eNpHR 3.0-EYFP (#26971). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-eNpHR 3.0-EYFP plasmid DNA. CaMKIIa-driven eNpHR 3.0-EYFP for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Promoter

   CaMKII

   Tags

   EYFP

   Available since

   March 30, 2018

   Availability

   Academic Institutions and Nonprofits only

8. Mouse sgRNA library Brie in lentiCRISPRv2

   Pooled Library

   #73632

   Purpose

   Mouse sgRNA library in backbone lentiCRISPRv2 containing 78,637 unique sgRNAs targeting 19,674 genes along with 1000 non-targeting controls

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Expression

   Mammalian

   Use

   CRISPR and Lentiviral

   Available since

   Feb. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pCDH-hMALAT1

   Plasmid

   #118580

   Purpose

   to overexpress human MALAT1 in mammalian cells

   Depositor

   Jianjun Zhao

   Article

   Hu et al Leukemia. 2018 Mar 22. pii: 10.1038/s413

   Insert

   MALAT1 (MALAT1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV

   Available since

   Oct. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pXAT2

    Plasmid

    #80494

    Purpose

    AAVS1 sgRNA expression vector

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Insert

    AAVS1 sgRNA-T2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pREDCas9

    Plasmid

    #71541

    Purpose

    Constitutive expression of Cas9, inducible expression of the Red recombineering system, and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.

    Depositor

    Tao Chen

    Article

    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10

    Inserts

    Cas9
    lambda red genes
    plasmid curing system
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pZR112_Lenti-SFFV-mCherry-2A-dCas9-VP64

    Plasmid

    #180263

    Purpose

    Lentiviral expression vector for dCas9-VP64 in mammalian cells

    Depositor

    Alexander Marson

    Article

    Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Nuclease dead Cas9

    Promoter

    SFFV

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTP298 His14-Avi-27xGS-SUMOEu-2xGS-anti ALFA tag nanobody

    Plasmid

    #199390

    Purpose

    Bacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag, biotin acceptor peptide (Avi), and SUMOEu tag

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Insert

    anti-ALFA nanobody
    

    Use

    Tags

    14xHis-Avi-SUMOEu

    Expression

    Bacterial

    Mutation

    Promoter

    T5-LacO

    Available since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAG1160 mCherry-FIREtag

    Plasmid

    #205164

    Purpose

    Expresses mCherry -FIREtag in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Insert

    mCherry-FIREtag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. [#GS5-RV] MitoTRACER-Donor

    Plasmid

    #233505

    Purpose

    Retroviral construct of the MitoTRACER genetic reporter to be expressed in the donor cells

    Depositor

    Simon Grelet

    Article

    Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

    Insert

    MitoTRACER-Donor
    

    Use

    Retroviral and Synthetic Biology

    Tags

    GFP11 and HA Tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. APEX2-V5-G3BP1_pTRE-G418

    Plasmid

    #202013

    Purpose

    expresses APEX2-G3BP1 in the mammalian cytosol under dox inducible promoter, lentiviral vector

    Depositor

    Alice Ting

    Article

    Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

    Insert

    APEX2-V5-G3BP1
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pTRE-Tight

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hSyn-hChR2(H134R)-EYFP (AAV5)

    Viral Prep

    #26973-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-hSyn-hChR2(H134R)-EYFP (#26973). In addition to the viral particles, you will also receive purified pAAV-hSyn-hChR2(H134R)-EYFP plasmid DNA. hSyn-driven, humanized channelrhodopsin H134R mutant fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Promoter

    Syn

    Tags

    EYFP

    Available since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

18. SpyCatcher003 S49C

    Plasmid

    #133448

    Purpose

    Expresses SpyCatcher003 S49C in bacterial cytoplasm, with a unique cysteine for chemical coupling

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    SpyCatcher003 S49C
    

    Use

    Tags

    GSSGS, His6, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Contains S49C mutation

    Promoter

    T7

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET29b-10xHis-Super TEV

    Plasmid

    #193833

    Purpose

    Expresses high-efficiency TEV protease in bacterial cells

    Depositor

    Florence Pojer, EPFL Protein Production and Structure Core Facility (PTPSP)

    Article

    Lau et al protocols.io, doi: 10.17504/protocols.io

    Insert

    TEV protease
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    T17S, L56V, N68D, I77V, S135G, I138T, S153N, T180…

    Promoter

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. Pro-Code Vector Kit

    Plasmid Kit

    #1000000177

    Purpose

    The Pro-Code Vector Kit is a set of 120 lentiviral vector plasmids, each with a unique protein barcode (Pro-Code) and gRNA cloning site.These plasmids can be used for CRISPR screens.

    Depositor

    Brian Brown

    Available since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only