We narrowed to 10,367 results for: ada

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pSV_238

Plasmid

#122941

Purpose

Expression of the repressor to control the insertional promoter.

Depositor

Attila Becskei

Article

Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

Insert

rTetR.SUM1

Expression

Yeast

Promoter

Pret2

Available Since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shNfs1

Plasmid

#102967

Purpose

Suppression of mNfs1

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shNfs1

Use

Lentiviral and RNAi

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pKS013

Plasmid

#65466

Purpose

pSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing mCherry

Depositor

Keith Tyo

Article

Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

Insert

mCherry

Use

Synthetic Biology

Expression

Bacterial

Promoter

pl-TetO

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only
pAWp9-2

Plasmid

#73853

Purpose

pFUGW-UBCp-RFP-CMVp-GFP-U6p-RFP-sg1

Depositor

Timothy Lu

Article

Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

Insert

UBCp-RFP-CMVp-GFP-U6p-RFP-sg1

Use

Lentiviral

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
pLIC.B4.BSU37660

Plasmid

#11535

Depositor

Sung-Hou Kim

Article

Xu et al J Struct Funct Genomics. 2005 Dec;6(4):2

Insert

phosphotransacetylase

Tags

HisTag-MBP, TEV cleavable

Expression

Bacterial

Available Since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
pATetO 6XHis T4 DNA Ligase

Plasmid

#206207

Purpose

Inducible expression of T4 DNA ligase

Depositor

Andrew Ellington

Article

Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

Insert

T4 DNA ligase

Expression

Bacterial

Available Since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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px459 EQR

Plasmid

#101732

Purpose

Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 EQR

Expression

Mammalian

Mutation

VQR

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pFRT_TO_FlagHA_USP10

Plasmid

#127102

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

USP10 (USP10 Human)

Expression

Mammalian

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pCas9/VRQR-sgRNA (BbsI)

Plasmid

#129725

Purpose

Expressing SpCas9/VRQR mutant and sgRNA for target sequence with NGA PAM

Depositor

Elina Ikonen

Article

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-BOLL

Plasmid

#222526

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of BOLL

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

BOLL (BOLL Human)

Expression

Mammalian

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pRSETB/mStayGold(c4)

Plasmid

#212018

Purpose

For N-terminal tagging with mStayGold. c4 is the appendage that facilitates fusions to the C-terminus of mStayGold.

Depositor

Atsushi Miyawaki

Article

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

mStayGold(c4)

Expression

Bacterial

Promoter

T7

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pAM5057

Plasmid

#120085

Purpose

Broad host range plasmid. Theophylline inducible expression of YFP in various cyanobacteria.

Depositor

Susan Golden

Article

Ma et al Appl Environ Microbiol. 2014 Nov;80(21):

Insert

RSF1010 Y25F aadA PconII theophylline riboswitch F with myc-yfp reporter

Use

Other

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pET29-bdSUMO-Anti-ALFA nanobody

Plasmid

#212311

Purpose

Expression of anti-ALFA nanobody

Depositor

Arthur Laganowsky

Article

Stover et al J Struct Biol X. 2024 Feb 2;9:100097. do

Insert

anti-ALFA nanobody

Tags

bdSUMO

Expression

Bacterial

Promoter

T7

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-C-dTAG-Puro

Plasmid

#207727

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Puro cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-N-Puro-dTAG

Plasmid

#207694

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-dTAG cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pEF1α-Cre-2A-Puro

Plasmid

#207743

Purpose

Expression of the Cre recombinase linked to a puro selectable marker

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

Cre-2A-Puro

Use

Cre/Lox

Expression

Mammalian

Promoter

EF1alpha

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-N-Zeo-dTAG

Plasmid

#207705

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-dTAG cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-C-dTAG-Zeo

Plasmid

#207738

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Zeo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pEF1α-Cre-2A-mTagBFP2

Plasmid

#207746

Purpose

Expression of the Cre recombinase linked to a mTagBFP2 fluorescent marker

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

Cre-PGK-mTagBFP2

Use

Cre/Lox

Expression

Mammalian

Promoter

EF1alpha

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pNHA7

Plasmid

#223649

Purpose

birA protein purification

Depositor

François-Xavier Campbell-Valois

Article

Haidar-Ahmad et al mSphere. 2024 Nov 21;9(11):e0055324. doi

Insert

BioID_G118R

Tags

6His

Expression

Bacterial

Mutation

G118R

Promoter

LacI

Available Since

Oct. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pGEMHE-vhhGFP4-hIgG1-Fc

Plasmid

#105576

Purpose

T7 promotor drives in vitro mRNA transcription of GFP nanobody-Fc antibody domain fusion

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

Human IgG1 Fc-domain fused with GFP nanobody (IGHG1 Human)

Use

In vitro transcription

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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ER-mScarletI

Plasmid

#137805

Purpose

Visualization of the Endoplasmic Reticulum

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

KDEL

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pEF1α-Cre-2A-mScarlet

Plasmid

#207745

Purpose

Expression of the Cre recombinase linked to a mScarlet fluorescent marker

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

Cre-PGK-mScarlet

Use

Cre/Lox

Expression

Mammalian

Promoter

EF1alpha

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pATetO 6XHis-Taq DNA Pol

Plasmid

#178782

Purpose

Inducible expression of Taq DNA polymerase in E. coli

Depositor

Andrew Ellington

Article

Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

Insert

Taq DNA Polymerase

Expression

Bacterial

Available Since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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qTAG-C-3xFLAG-Puro

Plasmid

#207728

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xFLAG-2A-Puro cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pLJM1 Flag Raptor

Plasmid

#26633

Depositor

David Sabatini

Article

Sancak et al Cell. 2010 Apr 16. 141(2):290-303.

Insert

raptor (RPTOR Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only

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pCAG-GCaMP6s

Plasmid

#171156

Purpose

Encodes GCaMP6s

Depositor

Hillel Adesnik

Article

Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

Insert

GCaMP6s

Expression

Mammalian

Available Since

Aug. 13, 2021

Availability

Academic Institutions and Nonprofits only

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qTAG-C-mNeon-Puro

Plasmid

#207720

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mNeon-2A-Puro cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
qTAG-C-moxGFP-Puro

Plasmid

#207721

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a moxGFP-2A-Puro cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDL278_P23-DsRed-Express2

Plasmid

#121505

Purpose

E. coli-streptococcal shuttle vector that expresses rfp

Depositor

Robert Shields

Article

Shields et al Appl Environ Microbiol. 2019 May 17. pii

Insert

DsRed-Express2

Use

E. coli streptococcal shuttle vector

Promoter

P23

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pRK-flag-USP19

Plasmid

#78597

Purpose

Express flag-USP19 in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-USP19 (USP19 Human)

Tags

flag

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCas9-sgAAVS1-1

Plasmid

#129726

Purpose

Expressing SpCas9 and sgAAVS1-1

Depositor

Elina Ikonen

Article

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Insert

SpCas9 and sgAAVS1-1

Use

CRISPR

Tags

P2A-Puro

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pMJ806

Plasmid

#39312

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Promoter

T7

Available Since

Sept. 7, 2012

Availability

Academic Institutions and Nonprofits only

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5HT6-mCherry-G-GECO1.0

Plasmid

#47500

Purpose

Fluorescent reporter for Ca2+ inside primary cilia with mCherry reference

Depositor

Takanari Inoue

Article

Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

Insert

G-GECO1.0

Tags

5HT6 and mCherry

Expression

Mammalian

Available Since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only

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PCMV4-3 HA/IkB-alpha(SS32,36AA)

Plasmid

#24143

Depositor

Warner Greene

Article

Sun et al Mol Cell Biol. 1996 Mar . 16(3):1058-65.

Insert

IkB alpha (NFKBIA Human)

Tags

3xHA

Expression

Mammalian

Mutation

S32A S36A

Available Since

March 25, 2010

Availability

Academic Institutions and Nonprofits only

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ER-mNeonGreen

Plasmid

#137804

Purpose

Visualization of the Endoplasmic Reticulum

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

KDEL

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MaLionG

Plasmid

#113906

Purpose

Green ATP sensor

Depositor

Tetsuya Kitaguchi

Article

Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

Insert

MaLionG

Expression

Mammalian

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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mNeptune-pBAD

Plasmid

#54714

Purpose

Localization: Protein Expression Vector , Excitation: 600, Emission: 650

Depositor

Robert Campbell, Michael Davidson, Nathan Shaner, Roger Tsien

Article

Lin et al Chem Biol. 2009 Nov 25;16(11):1169-79. d

Type

Empty backbone

Tags

6xHis and mNeptune

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pATetO 6XHis-Taq DNA Ligase

Plasmid

#178798

Purpose

Inducible expression of Taq DNA Ligase in E. coli

Depositor

Andrew Ellington

Article

Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

Insert

Taq DNA Ligase

Expression

Bacterial

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST14-DogCatcher

Plasmid

#171772

Purpose

Expresses DogCatcher protein in bacterial cytoplasm

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

DogCatcher

Tags

His6 and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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