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We narrowed to 1,333 results for: Ncl

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Showing: 1141 - 1160 of 1333 results

  1. pAAV-hSyn-Gluc-feRMA-IRES-EGFP

    Plasmid
    #248190
    Purpose
    Expresses Gluc-feRMA and EGFP under the hSyn promoter. Gluc-feRMA enables monitoring of neuronal transduction and includes a TEV cs, allowing inactivation of it upon interaction with TEV protease.
    Depositor
    Jerzy Szablowski
    Article
    Nouraein et al Proc Natl Acad Sci U S A. 2025 Dec 9;122
    Inserts
    Gaussia luciferase with TEV cleavage site fused to IgG1 Fc
    IRES-EGFP
    Use
    AAV; Luciferase
    Tags
    Gluc (N terminal on insert) and IgG1 Fc (C termin…
    Expression
    Mammalian
    Promoter
    IRES and hSyn
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    248190-plasmid-map-sequence-id-495612

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  2. pDestTol2CR-QUASM1:GCaMP7s-NLS-P2A-mKate2-NLS

    Plasmid
    #241915
    Purpose
    Zebrafish transgenesis plasmid encoding GCaMP7s-NLS and mKate2-NES separated by the P2A peptide, driven by the QUASM1 enhancer, and including a cmlc2:mKate2 cardiac transgenesis marker
    Depositor
    Balázs Enyedi
    Article
    Fazekas et al J Cell Biol. 2026 Jan 5;225(1):e20250116
    Insert
    QUASM1 enhancer driving GCaMP7s-NLS and mKate2-NLS
    Use
    Zebrafish tol2 transgenesis
    Promoter
    QUASM1
    Available Since
    Oct. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    241915-plasmid-map-sequence-id-487161

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  4. eft-3p:act-5(ptc):tbb2 3' UTR

    Plasmid
    #202331
    Purpose
    The ubiquitous eft-3 promoter drives overexpression of the mutant act-5(ptc) gene including introns.
    Depositor
    Didier Stainier
    Article
    Welker et al PLoS Genet. 2023 Jun 29;19(6):e1010806.
    Inserts
    eft-3 promoter
    act-5(ptc)
    tbb-2 3' UTR
    Tags
    base 238 C -> A mutation causing a PTC at codo…
    Expression
    Worm
    Promoter
    This is a promoter sequence for C. elegans eft-3,…
    Available Since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    202331-plasmid-map-sequence-id-396314

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  7. pBetaActin-FKBP-Halo-Dync1h1motor

    Plasmid
    #191333
    Purpose
    Expresses the fluorescently labeled dynein motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB
    Depositor
    Frank Bradke
    Article
    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:
    Insert
    FKBP-Halo-Dync1h1motor (Dync1h1 Mouse)
    Expression
    Mammalian
    Mutation
    DYNC1H1 motor domain aa 1453-4644
    Available Since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    191333-plasmid-map-sequence-id-381754

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  12. vPyACR_2164382

    Plasmid
    #153029
    Purpose
    Anion-conducting channelrhodopsin of viral origin. Codon-optimized for mammalian expression (human/mouse).
    Depositor
    Peter Hegemann
    Article
    Rozenberg et al Curr Biol. 2020 Dec 21;30(24):4910-4920.
    Insert
    vPyACR_2164382
    Tags
    eYFP
    Expression
    Mammalian
    Mutation
    vPyACR_2164382 was C-terminally truncated to incl…
    Promoter
    CMV (+ enhancer)
    Available Since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    153029-plasmid-map-sequence-id-300511

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  13. vPyACR_21821

    Plasmid
    #153030
    Purpose
    Anion-conducting channelrhodopsin of viral origin. Codon-optimized for mammalian expression (human/mouse).
    Depositor
    Peter Hegemann
    Article
    Rozenberg et al Curr Biol. 2020 Dec 21;30(24):4910-4920.
    Insert
    vPyACR_21821
    Tags
    eYFP
    Expression
    Mammalian
    Mutation
    vPyACR_21821 was C-terminally truncated to includ…
    Promoter
    CMV (+ enhancer)
    Available Since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    153030-plasmid-map-sequence-id-300513

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  16. pAN19-ccvTIR1-GUS-NOSt

    Plasmid
    #108548
    Purpose
    Cloning vector including C-terminally GUS-tagged Arabidopsis auxin receptor TIR1 with the F79G mutation [ccvTIR1] and NOS terminator
    Depositor
    Keiko Torii
    Article
    Uchida et al Nat Chem Biol. 2018 Mar;14(3):299-305. d
    Insert
    TRANSPORT INHIBITOR RESPONSE 1 fused with GUS and NOS terminator (TIR1 Mustard Weed)
    Use
    Cloning vector
    Tags
    GUS
    Mutation
    Changed F79 to G
    Available Since
    May 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    108548-plasmid-map-sequence-id-210051

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  18. pHSN501

    Plasmid
    #50589
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    zCas9D10A
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG and NLS
    Mutation
    Cas9D10A nickase, was derived from the zCas9 and …
    Promoter
    2×35Sp and AtU6-26p
    Available Since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50589-plasmid-map-sequence-id-75662

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  20. MLRV

    Plasmid
    #118069
    Purpose
    Multi-luciferase reporter vector including transcriptional reporters for NF-kb, TGF-b, c-Myc, p53 and MAPK/JNK transcriptional reporters
    Depositor
    Koen Venken
    Article
    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:
    Inserts
    TB:5xNF-κβ:RedF:bGHpA
    TB:4xTGF-β:FLuc:bGHpA
    TB:5xE-box:Renilla:bGHpA
    TB:2xp53:NLuc:bGHpA
    TB:6xAP-1:GrRenilla:bGHpA
    CMV:ELuc:bGHpA
    Use
    Luciferase and Synthetic Biology; Assembly of gb2…
    Expression
    Mammalian
    Promoter
    2xp53::miniP, 4xTGF-B::miniP, 5xEbox::miniP, 5xNF…
    Available Since
    Dec. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    118069-plasmid-map-sequence-id-240503

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Showing: 1141 - 1160 of 1333 results