We narrowed to 1,221 results for: poli

1. tet-pLKO.puro_shGFP

   Plasmid

   #110421

   Purpose

   shGFP control, silence GFP gene, doxycycline inducible, puromycin selection

   Depositor

   Sandra Martha Gomes Dias

   Insert

   GFP
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1/TO (RNA PolIII)

   Available since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. tet-pLKO.puro_shLuc

   Plasmid

   #110422

   Purpose

   shLuc control, silence Luc gene, doxycycline inducible, puromycin selection

   Depositor

   Sandra Martha Gomes Dias

   Insert

   Luciferase
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1/TO (RNA PolIII)

   Available since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRS414-7x2-PHO5-GFP-hPLC delta PH domain dimer

   Plasmid

   #58837

   Purpose

   Expresses GFP-Tagged Human PLC delta PH domain in yeast

   Depositor

   Tim Levine

   Insert

   2x Phospolipase C delta1 PH domain (Plcd1 Rat)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   PHO5

   Available since

   Sept. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pSAV108

   Plasmid

   #226323

   Purpose

   Empty backbone plasmid for genomic insertions into H. neapolitanus neutral site

   Depositor

   David Savage

   Article

   Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO.1-blast shLuc

   Plasmid

   #110409

   Purpose

   shLuc (Target TTACGCTGAGTACTTCGA), silence LUC gene, blasticidin selection.

   Depositor

   Sandra Martha Gomes Dias

   Insert

   Luciferase
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 (RNA PolIII)

   Available since

   Nov. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. tet-pLKO.puro_shHuR CDS

   Plasmid

   #110411

   Purpose

   TRCN0000276129 (Target CGAGCTCAGAGGTGATCAAAG), silence human ELAVL1 (HuR) gene, doxycycline inducible, puromycin selection

   Depositor

   Sandra Martha Gomes Dias

   Insert

   ELAVL1 (HuR)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1/TO (RNA PolIII)

   Available since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. tet-pLKO.puro_shGAC

   Plasmid

   #110423

   Purpose

   shGAC, silence glutaminase GAC isoform, doxycycline inducible, puromycin selection

   Depositor

   Sandra Martha Gomes Dias

   Insert

   GLS glutaminase (GLS Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1/TO (RNA PolIII)

   Available since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. tet-pLKO.puro_shHuR 3UTR

   Plasmid

   #110412

   Purpose

   TRCN0000276186 (Target TTGTTAGTGTACAACTCATTT), silence human ELAVL1 (HuR) gene, doxycycline inducible, puromycin selection

   Depositor

   Sandra Martha Gomes Dias

   Insert

   ELAVL1 (HuR)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1/TO (RNA PolIII)

   Available since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLKO.puro_shHuR 3UTR

   Plasmid

   #110414

   Purpose

   TRCN0000276186 (Target TTGTTAGTGTACAACTCATTT), silence human ELAVL1 (HuR) gene, puromycin selection

   Depositor

   Sandra Martha Gomes Dias

   Insert

   ELAVL1 (HuR)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 (RNA PolIII)

   Available since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. lentiGuide-Puro-pKO-library

    Pooled Library

    #190744

    Purpose

    CRISPR/Cas9 paired-guide RNA library to delete proximal poly(A) sites in mouse melanoma.

    Depositor

    Robert Bradley

    Article

    Gabel et al Nat Commun. 2024 Feb 1;15(1):959. doi: 1

    Expression

    Mammalian

    Species

    Mus musculus

    Use

    CRISPR, Lentiviral, and Mouse Targeting

    Available since

    Feb. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

11. pMSP2N2

    Plasmid

    #29520

    Depositor

    Stephen Sligar

    Article

    Grinkova et al Protein Eng Des Sel. 2010 Nov . 23(11):8

    Insert

    MSP2N2
    

    Use

    Tags

    7-His tag

    Expression

    Bacterial

    Mutation

    tandem MSP; His-tag on N-terminus followed by spa…

    Promoter

    Available since

    May 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBAD24-sfGFPx2

    Plasmid

    #51559

    Purpose

    Superfolder GFP ORF cloned into pBAD24 for expression in E. coli. It contains additional aminoacids in the N and C terminus (polilinker sequences for cloning proteins in frame with GFP)

    Depositor

    Sankar Adhya, Francisco Malagon

    Article

    Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261

    Insert

    superfolder GFP
    

    Use

    Tags

    GLESTCRHASLAVLADERRFSA and MARARA

    Expression

    Bacterial

    Mutation

    Contains additonal aa in the N and C terminus (in…

    Promoter

    Available since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFS462

    Plasmid

    #89065

    Purpose

    his7 integration plasmid with Z3EVpr:GFP

    Depositor

    Nick Rhind

    Article

    Ohira et al Yeast. 2017 Aug;34(8):323-334. doi: 10.1

    Insert

    Z3EV promoter GFP-NLS leu1 C-terminal 300 nt
    

    Use

    S. pombe his7 integration vector

    Tags

    Expression

    Mutation

    Promoter

    Z3EV

    Available since

    July 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLiLucB

    Plasmid

    #31596

    Depositor

    Michele Calos

    Article

    Woodard et al PLoS One. 2010 . 5(6):e11367.

    Insert

    alpha-1 antitrypsin promoter with an apolipoprotein enhancer (SERPINA1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBEAST_J23101-amidase

    Plasmid

    #128135

    Purpose

    The cell-free adapted backbone, pBEAST, expressing the amidase enzyme gene (benzamid to benzoate) under control of the constitutive promoter J23101, and RBS B0032

    Depositor

    Jean-Loup Faulon

    Article

    Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

    Insert

    amidase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23101

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. rat APOBEC1

    Plasmid

    #112858

    Purpose

    plasmid for expression of rat APOBEC1 in mammalian cells

    Depositor

    Silvestro Conticello

    Article

    Severi et al RNA Biol. 2015;12(4):389-97. doi: 10.108

    Insert

    APOBEC1 (Apobec1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Oct. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. rat APOBEC1 E63A

    Plasmid

    #112861

    Purpose

    plasmid for expression of a catalytically inactive rat APOBEC1 mutant in mammalian cells

    Depositor

    Silvestro Conticello

    Article

    Chieca et al bioRxiv 2021

    Insert

    APOBEC1 (Apobec1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Glutamic acid 63 to Alanine

    Promoter

    CMV promoter

    Available since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a-MSP1D1delltaH5

    Plasmid

    #71714

    Purpose

    Membrane scaffold protein (MSP), helix 5 deletion

    Depositor

    Gerhard Wagner

    Article

    Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

    Insert

    Apolipoprotein A1 (APOA1 Human)
    

    Use

    Tags

    6xHis and TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a-MSP1D1deltaH4-H6

    Plasmid

    #71717

    Purpose

    Membrane scaffold protein (MSP), helix 4 to 6 deletion

    Depositor

    Gerhard Wagner

    Article

    Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

    Insert

    Apolipoprotein A1 (APOA1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-MSP1D1deltaH4H5

    Plasmid

    #71716

    Purpose

    Membrane scaffold protein (MSP), helix 4+ helix 5 deletion

    Depositor

    Gerhard Wagner

    Article

    Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

    Insert

    Apolipoprotein A1 (APOA1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge