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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 62,376 results for: promoter

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Showing: 1141 - 1160 of 62376 results

  1. pAC152-dual-dCas9VP64-sgExpression

    Plasmid
    #48238
    Purpose
    Dual expression construct expressing both dCas9VP64 and sgRNA from separate promoters
    Depositor
    Rudolf Jaenisch
    Publication
    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9
    Use
    CRISPR
    Tags
    HA Tag, HA-Tag, and VP64
    Expression
    Mammalian
    Mutation
    D10A;H840A
    Available since
    Sept. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    48238-plasmid-map-sequence-id-69545

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  2. Flag-TurboID

    Plasmid
    #124646
    Purpose
    Expresses Flag-TurboID fusion for proximity labeling
    Depositor
    FENG-QIAN Li, Ken-Ichi Takemaru
    Type
    Empty backbone
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    124646-plasmid-map-sequence-id-435118

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  4. pCMV-GCaMP5G

    Plasmid
    #31788
    Purpose
    a single-wavelength GCaMP3-based calcium indicator with improved response. Please also see the GCaMP6s/m/f indicators.
    Depositor
    Douglas Kim, Loren Looger, GENIE Project
    Publication
    Akerboom et al J Neurosci. 2012 Oct 3;32(40):13819-40.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GCaMP5G
    Tags
    6xHis, T7 epitope, and Xpress tag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 22, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    31788-plasmid-map-sequence-id-228108

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  6. pAAV.hSyn.iGluSnFr.WPRE.SV40

    Plasmid
    #98929
    Purpose
    AAV expression of glutamate sensor from human synapsin promoter. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Publication
    Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:
    Has service
    AAV1, AAV5, and AAV9
    Insert
    iGluSnFr
    Use
    AAV
    Expression
    Mammalian
    Promoter
    hSyn
    Available since
    Jan. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    98929-plasmid-map-sequence-id-190089

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  8. pAAV.Syn.NES-jRGECO1a.WPRE.SV40 (AAV9)

    Viral prep
    #100854-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV.Syn.NES-jRGECO1a.WPRE.SV40 (#100854). In addition to the viral particles, you will also receive purified pAAV.Syn.NES-jRGECO1a.WPRE.SV40 plasmid DNA. Synapsin-driven GECO1a calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Publication
    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:
    Promoter
    Syn
    Available since
    April 17, 2018
    Availability
    Academic Institutions and Nonprofits only

  11. pcDNA3.1/hChR2(H134R)-mCherry

    Plasmid
    #20938
    Purpose
    Mammalian expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activation
    Depositor
    Karl Deisseroth
    Publication
    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    channelrhodopsin-2
    Expression
    Mammalian
    Mutation
    H134R mutation in ChR2
    Promoter
    CMV
    Available since
    June 1, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    20938-plasmid-map-sequence-id-181022

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  14. lenti sgRNA(MS2)_puro backbone

    Plasmid
    #73795
    Purpose
    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Publication
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    73795-plasmid-map-sequence-id-181202

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  19. pCMV-PEmax-P2A-GFP

    Plasmid
    #180020
    Purpose
    Mammalian expression of SpCas9 PEmax prime editor with P2A-EGFP marker
    Depositor
    David Liu
    Publication
    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PEmax-P2A-EGFP
    Tags
    P2A-EGFP, SV40 bpNLS, and c-Myc NLS
    Expression
    Mammalian
    Mutation
    Detailed in manuscript
    Promoter
    CMV
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    180020-plasmid-map-sequence-id-352752

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  20. pCMV(MinDis).iGluSnFR

    Plasmid
    #41732
    Purpose
    Single-wavelength extracellular glutamate sensor constructed from E. coli Gltl and cpGFP. Membrane-displayed. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Publication
    Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GltI based Glutamate Biosensor
    Use
    Glutamate biosensor
    Tags
    Myc, PDGFR transmembrane helix (aa 513-561), and …
    Expression
    Mammalian
    Mutation
    cpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…
    Promoter
    CMV
    Available since
    Jan. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    41732-plasmid-map-sequence-id-210671

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Showing: 1141 - 1160 of 62376 results