We narrowed to 59,253 results for: {{{s}}}

1. Nanog-luciferase targeting construct

   Plasmid

   #26591

   Depositor

   Rudolf Jaenisch

   Article

   Lyssiotis et al Proc Natl Acad Sci U S A. 2009 Jun 2. 10

   Insert

   Nanog promoter-luciferase-lox-pgk-neomycin-loc
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSLQ2822 pPB: CAG-GID1-KRAB-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9-ABI

   Plasmid

   #106895

   Purpose

   Expresses Sp dCas9 and GA-inducible KRAB for NOR gate/diametric regulation

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   GAI-tagBFP-Sp dCas9-ABI
   GID1-KRAB
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   2xNLS (SV40), ABI, GAI, GID1, HA tag, IRES-Puro, …

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and PGK

   Available Since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. Ai39

   Plasmid

   #34884

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Nat Neurosci. 2012 Mar 25;15(5):793-802.

   Insert

   CAG-Floxed eNpHRv3.0-EYFP
   

   Use

   Mouse Targeting

   Tags

   EYFP

   Expression

   Mutation

   Promoter

   CAG

   Available Since

   Feb. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAFNF-PSDd1.2-GFP

   Plasmid

   #125581

   Purpose

   Flp-dependent expression of PSD d1.2-GFP (a deletion mutant of PSD-95 fused to GFP) in mammalian cells.

   Depositor

   Takeshi Imai

   Article

   Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

   Insert

   PSDd1.2-GFP (Dlg4 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. FR_HNF4A2-C106R

   Plasmid

   #31123

   Depositor

   Gerhart Ryffel

   Article

   Erdmann et al Biol Chem. 2007 Jan . 388(1):91-106.

   Insert

   HNF4A (HNF4A Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   splice variant 2, mutation C106R. Wild type seq…

   Promoter

   Available Since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCIG AKT3 S472E

   Plasmid

   #73053

   Purpose

   Human AKT3 S472E

   Depositor

   Joseph Gleeson

   Article

   Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

   Insert

   AKT3 S472E (AKT3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMXs-hs-3xHA-deltaRING-LIN-41

   Plasmid

   #52718

   Purpose

   retroviral expression of human LIN-41 / TRIM71 containing a RING domain deletion and 3 N-terminal HA tags

   Depositor

   Shinya Yamanaka

   Article

   Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

   Insert

   LIN-41 (TRIM71 Human)
   

   Use

   Retroviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   deleted amino acids 12-91

   Promoter

   Available Since

   June 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. mEos3-caRac1

   Plasmid

   #92041

   Purpose

   Expresses mEos3 fusion with caRac1 (Q61L)

   Depositor

   Ji Yu

   Article

   Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

   Insert

   RAC1 (RAC1 Human)
   

   Use

   Tags

   mEos3

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Q61L (constitutively active)

   Promoter

   CMV

   Available Since

   June 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pv9_MBD1_MBD-WT-13XL-BASU

   Plasmid

   #179416

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1.MBD-WT-13XL-BASU

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   MBD1.MBD-WT-13XL-BASU
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pet23a_RBPMS_FL

    Plasmid

    #59392

    Purpose

    Plasmid for bacterial overexpression of RBPMS

    Depositor

    Thomas Tuschl

    Article

    Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

    Insert

    RBPMS (RBPMS Human)
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. LbCas12a-DR-GC-swap-cr in pBCSK+

    Plasmid

    #126643

    Purpose

    Cloning vector LbCas12a-DR-GC-swap-crRNA for expression in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

    Insert

    LbCas12a Full Length Direct Repeat GC Swap crRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 Promoter

    Available Since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRS0035

    Plasmid

    #131125

    Purpose

    Mammalian Target-AID expression vector

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    nCas9-PmCDA1-UGI
    

    Use

    CRISPR

    Tags

    PmCDA1 and UGI

    Expression

    Mammalian

    Mutation

    D10A in SpCas9

    Promoter

    CMV promoter

    Available Since

    Sept. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO.1P POLD1_1

    Plasmid

    #160791

    Purpose

    Suppress POLD1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLD1_1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO.1P REV3L_2

    Plasmid

    #160798

    Purpose

    Suppress REV3L

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shREV3L_2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLKO.1P REV3L_1

    Plasmid

    #160797

    Purpose

    Suppress REV3L

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shREV3L_1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLKO.1P POLA1_2

    Plasmid

    #160790

    Purpose

    Suppress POLA1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLA1_2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLKO.1P POLA1_1

    Plasmid

    #160789

    Purpose

    Suppress POLA1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLA1_1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMSCV-puro-AS9 (Mito MTS)-Delta N67-mMCL1

    Plasmid

    #45822

    Depositor

    Joseph Opferman

    Article

    Perciavalle et al Nat Cell Biol. 2012 Apr 29;14(6):575-83.

    Insert

    AS9(Mito MTS)-Delta N67-mMCL1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    ATP Synthase subunit 9 is used as a Mito Targetin…

    Promoter

    Available Since

    July 24, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pET28_6xHis-PARP1delWGR-L713F

    Plasmid

    #173941

    Purpose

    Bacterial expression of PARP1 lacking WGR domain but containing L713F gain-of-function mutation

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1delWGR-L713F (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Deletion of residues 521-660; L713F, V762A

    Promoter

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. PA-DreN

    Plasmid

    #169707

    Purpose

    The optimized DreN246-nMag

    Depositor

    Dali Li

    Article

    Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

    Insert

    Dre246-nMag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CAG

    Available Since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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