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pAAVone-AAV2-CMV-eGFP

Plasmid

#230930

Purpose

pAAVone plasmid is used to package AAV-CMV-eGFP with an AAV2 serotype, utilizing the AAVone single-plasmid AAV production system.

Depositor

Qizhao Wang

Article

Yang et al Mol Ther Nucleic Acids. 2025 May 14;36(2

Insert

eGFP

Use

AAV

Promoter

CMV

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCMMP-MCS-IRES-eGFP-P2A-iCas9

Plasmid

#178533

Purpose

For genome editing: EGFP-inducible Caspase9 knock-in cassette which allows ablation of target cells

Depositor

Ryoji Yao

Article

Okamoto et al STAR Protoc. 2021 Sep 16;2(4):100780. do

Insert

iCaspase9 (CASP9 Human)

Expression

Mammalian

Mutation

FKBP12 F36 to V Truncated Caspase9 (135-416; lack…

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pmTurquoise2-N1

Plasmid

#60561

Purpose

Expresses the cyan fluorescent protein variant mTurquoise2, can be used to create fusion proteins

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-BE3

Plasmid

#73021

Purpose

Expresses BE3 in mammalian cells

Depositor

David Liu

Article

Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

Insert

BE3

Expression

Mammalian

Promoter

CMV

Available Since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

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N-Cadherin-EGFP

Plasmid

#18870

Depositor

Valeri Vasioukhin

Article

Nechiporuk et al Dev Cell. 2007 Sep . 13(3):338-50.

Insert

N-Cadherin (Cdh2 Mouse)

Tags

EGFP, Kanamycin, and N-Cadherin

Expression

Mammalian

Available Since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only

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pJL1-mCherry

Plasmid

#102629

Purpose

In vitro expression of mCherry from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

mCherry

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pJOE7706.1

Plasmid

#135075

Purpose

A shuttle vector for heterologous HA production with Corynebacterium glutamicum

Depositor

Josef Altenbuchner

Article

Hoffmann et al J Appl Microbiol. 2014 Sep;117(3):663-78

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-SpRY-P2A-EGFP (RTW4830)

Plasmid

#139989

Purpose

CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Walton et al Science. 2020 Apr 17;368(6488):290-296.

Insert

human codon optimized SpCas9 variant named SpRY with BPNLS-3xFLAG-P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

Promoter

CMV and T7

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pMPRAdonor2

Plasmid

#49353

Purpose

minP-luc2 donor plasmid for MPRA library construction

Depositor

Tarjei Mikkelsen

Article

Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

Insert

luc2

Use

Luciferase

Promoter

minP

Available Since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV.TBG.PI.Null.bGH

Plasmid

#105536

Purpose

empty AAV vector with TBG promoter

Depositor

James M. Wilson

Has Service

AAV8

Insert

None

Use

AAV

Expression

Mammalian

Promoter

TBG

Available Since

Feb. 2, 2018

Availability

Academic Institutions and Nonprofits only

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