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AAV.hSyn.GLPLight-ctr

Plasmid

#187468

Purpose

Expresses GLPLight-ctr in mammalian cells

Depositor

Tommaso Patriarchi

Article

Duffet et al Elife. 2023 Jun 2;12:RP86628. doi: 10.75

Insert

GLPLight-ctr

Use

AAV

Tags

Flag tag

Promoter

human Synapsin-1

Available Since

Feb. 16, 2023

Availability

Academic Institutions and Nonprofits only

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LZF39: Cre-off hSyn-CheRiff-CFP (FAS)

Plasmid

#104118

Purpose

synOptopatch construct

Depositor

Adam Cohen

Article

Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

Insert

CheRiff-CFP

Use

Lentiviral

Expression

Mammalian

Promoter

human synapsin I

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pLncEXP

Plasmid

#64865

Purpose

Empty vector for lncRNA expression

Depositor

Lei Sun

Article

Alvarez-Dominguez et al Cell Metab. 2015 May 5;21(5):764-76. doi

Type

Empty backbone

Use

Retroviral

Promoter

CMV

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP-ninein C-ter

Plasmid

#73523

Purpose

Encodes the C-terminus of mouse ninein (residues 1874-2113) with an EGFP tag at its N-terminus

Depositor

Michel Bornens

Article

Delgehyr et al J Cell Sci. 2005 Apr 15;118(Pt 8):1565-7

Insert

ninein C-ter (residues 1874-2113) (Nin Mouse)

Tags

EGFP tag

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA5-FRT-TetO-U2B''-fSNAPc

Plasmid

#106988

Purpose

open reading frame of U2B'' inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sites

Depositor

Melissa Moore

Article

Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

Insert

UB2" (SNRPB2 Human)

Tags

fSNAP

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pT-GEM(2)

Plasmid

#62894

Purpose

Trojan-Gal4 Expression Module in Phase 2. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Type

Empty backbone

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pCoofy1-psHaloTag1a

Plasmid

#232940

Purpose

Photoswichable HaloTag: psHaloTag1a variant

Depositor

Claire Deo

Article

Walterspiel et al Angew Chem Int Ed Engl. 2025 Oct 23:e202

Insert

photoswitchable HaloTag 1a variant

Tags

His6x-HRV3C

Expression

Bacterial

Mutation

E143W, A258W with linkers L1 (FAG) and L2 (P)

Promoter

T7

Available Since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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inert_EJC

Plasmid

#216224

Purpose

GFP P2A RFP reporter with inert EJC immediately following stop codon

Depositor

Rebecca Voorhees

Article

Inglis et al J Cell Sci. 2023 May 15;136(10):jcs26121

Insert

GFP P2A RFP_inert_EJC

Expression

Mammalian

Available Since

April 15, 2024

Availability

Academic Institutions and Nonprofits only

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pVB3011

Plasmid

#113389

Purpose

Galactose-inducible L-BC gag: cure killer

Depositor

Suzanne Sandmeyer

Article

Bilanchone et al PLoS Genet. 2015 Sep 30;11(9):e1005528.

Insert

L-BC Gag

Expression

Yeast

Mutation

Please see depositor comments

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pMXs-hNeuroD1

Plasmid

#233184

Purpose

Retroviral expression of NeuroD1

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

NeuroD1 (NEUROD1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-K-I-N-CAAX

Plasmid

#41640

Purpose

Expresses caged Cdc42 GEF intersectin: Intersectin Dbl homology (DH) domain fused to wild-type Dronpa145K at C terminus and Dronpa145N at N terminus, for optogenetic activation

Depositor

Michael Lin

Article

Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi

Insert

Dronpa145K-IntersectinDH-Dronpa145N-CAAX

Tags

CAAX box

Expression

Mammalian

Mutation

K145N on Dronpa

Promoter

CMV

Available Since

Dec. 21, 2012

Availability

Academic Institutions and Nonprofits only

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TC374

Plasmid

#122614

Purpose

Prpl-28::rtetR-QFAD::P2A::mKate::T2A::tetR-pie1

Depositor

Tian Chi

Article

Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

Insert

rtetR-QFAD::P2A::mKate::T2A::tetR-pie1

Expression

Worm

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMN-013

Plasmid

#195417

Purpose

AAVS1 Integratable mCherry Reporter for synZiFTR ZF5

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

4X ZF5 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pA5c-tesA-vhb50-8fadR

Plasmid

#86601

Purpose

expression of tesA and Vhb50 under the control of a LacUV5 promoter and fadR under the control of a pBAD promoter

Depositor

Yinjie Tang

Article

Liu et al Biotechnol Bioeng. 2016 Aug 6. doi: 10.1

Inserts

tesA
Vhb50
fadR

Expression

Bacterial

Promoter

LacUV5 and pBAD

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pCDH-anti-mCherry(LaM6) PAGER(Gi)

Plasmid

#230018

Purpose

Lentiviral vector expressing anti-mCherry PAGER(Gi) in mammalian cells

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

MT1-LaM6-TEVcs-ALFA-hM4D

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pCDH-SFFV-anti-mCherry(LaM6) PAGER(Gi)

Plasmid

#230021

Purpose

Lentiviral vector expressing anti-mCherry PAGER(Gi) in human T cells

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

MT1-LaM6-TEVcs-ALFA-hM4D

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-PS Intein

Plasmid

#242020

Purpose

AAV version of PS Intein palsmid.

Depositor

Vladislav Verkhusha

Article

Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

Insert

PS Intein

Use

Adenoviral

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pUAP4

Plasmid

#136079

Purpose

Acceptor plasmid for L0 parts. Clone L0 parts into pUAP4 with SapI, lacZ blue-white screening. Release parts to assemble transcription units with BsaI.

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Use

Synthetic Biology

Expression

Plant

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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(R42A) Cyclin B1-GFP (62)

Plasmid

#61849

Purpose

mammalian expression of Cyclin B1 with R42A mutation in destruction box to make cyclin B1 non-destructable

Depositor

Jonathon Pines

Article

Clute et al Nat Cell Biol. 1999 Jun;1(2):82-7.

Insert

cyclin B1 (CCNB1 Human)

Tags

GFP and Myc

Expression

Mammalian

Mutation

V5A R42A

Promoter

CMV

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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GFAP-Arclight

Plasmid

#187978

Purpose

Expresses the fluorescent voltage indicator Arclight under the GFAP promoter

Depositor

Chris Dulla

Article

Armbruster et al Nat Neurosci. 2022 May;25(5):607-616. do

Insert

Arclight A242

Use

AAV

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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