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pAAV.CAG.(mem).iGlucoSnFR2

Plasmid

#244077

Purpose

Plasma membrane targeted expression of green glucose sensor

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2

Use

AAV

Tags

IgG kappa leader

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pTRIP-CMV-GFP-FLAG-cGAS

Plasmid

#86675

Purpose

Expresses catalytically-active GFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.

Depositor

Nicolas Manel

Article

Raab et al Science. 2016 Apr 15;352(6283):359-62. d

Insert

GFP-FLAG-cGAS

Use

Lentiviral

Available Since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-TetON-3G

Plasmid

#96963

Purpose

Mammalian expression of Clontech rtTA driven by human beta-actin promoter

Depositor

Elena Cattaneo

Article

Faedo et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

Type

Empty backbone

Expression

Mammalian

Available Since

Aug. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-bJun-C-mSOG-IRES-mCherry

Plasmid

#136600

Purpose

Encodes bJun domain tagged with the C-terminal fragment of split-miniSOG (mSOG-Ja95-140).

Depositor

John Ngo

Article

Boassa et al Cell Chem Biol. 2019 Oct 17;26(10):1407-

Insert

bJun-C-mSOG

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-N-mSOG-bFos-IRES-mCherry

Plasmid

#136599

Purpose

Encodes bFos domain tagged with the N-terminal fragment of split-miniSOG (mSOG1-94).

Depositor

John Ngo

Article

Boassa et al Cell Chem Biol. 2019 Oct 17;26(10):1407-

Insert

N-mSOG-bFos

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2021

Availability

Academic Institutions and Nonprofits only

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pTRIP-CMV-tagRFP-FLAG-cGAS

Plasmid

#86676

Purpose

Expresses catalytically-active tagRFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.

Depositor

Nicolas Manel

Article

Raab et al Science. 2016 Apr 15;352(6283):359-62. d

Insert

tagRFP-FLAG-cGAS

Use

Lentiviral

Available Since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

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TRE-UBC-HLA-E SCT-T2A-GFP

Plasmid

#205461

Purpose

lentiviral plasmid for expression of HLA-E SCT

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

HLA-E SCT (HLA-E Human)

Expression

Mammalian

Promoter

UBC

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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phOct4-EGFP

Plasmid

#38776

Depositor

Wei Cui

Article

Gerrard et al Stem Cells. 2005;23(1):124-33.

Insert

Oct-4 promoter (POU5F1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

human OCT4

Available Since

Dec. 13, 2012

Availability

Academic Institutions and Nonprofits only

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H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen

Plasmid

#129631

Purpose

Visualization of Akt and ERK activity

Depositor

Dorus Gadella

Article

Chavez-Abiega et al J Cell Sci. 2022 Mar 15;135(6):jcs259685

Insert

H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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XLone-BSD SPI1

Plasmid

#179514

Purpose

Tunable and temporal expression control of SPI1

Depositor

Xiaoping Bao

Article

Jung et al ACS Synth Biol. 2022 May 24. doi: 10.102

Insert

SPI1 (SPI1 Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE3GS Promoter

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO eNpHR 3.0-EYFP

Plasmid

#26966

Purpose

AAV expression of eNpHR 3.0 fused to EYFP driven by EF1a promoter for optogenetic inhibition

Depositor

Karl Deisseroth

Article

Gradinaru et al Cell. 2010 Apr 2. 141(1):154-65.

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

eNpHR 3.0

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

ER export at 3' end of gene. Trafficking sig…

Promoter

EF1alpha

Available Since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only

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hMLKL-Venus

Plasmid

#106078

Purpose

expression in mammalian cells

Depositor

Douglas Green

Article

Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

Insert

human MLKL-Venus (MLKL Human)

Use

Retroviral; Tet-on retrovirus

Tags

Venus

Expression

Mammalian

Promoter

Dox-inducible

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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Oct4-IRES-eGFP-PGK-Neo

Plasmid

#48681

Purpose

For knock-in donor vector by CRISPR/CAS system

Depositor

Rudolf Jaenisch

Article

Yang et al Cell. 2013 Aug 27. pii: S0092-8674(13)01

Insert

Oct4 (Pou5f1 Mouse)

Use

CRISPR

Tags

GFP

Available Since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pmCherry-NLS

Plasmid

#39319

Depositor

Martin Offterdinger

Article

Micutkova et al Int J Cancer. 2012 Apr 1;130(7):1544-57.

Insert

mCherry-3xNLS

Tags

3xSV40LT NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 26, 2012

Availability

Academic Institutions and Nonprofits only

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PB-REVERBA-mScarlet-NLS-PEST

Plasmid

#190658

Purpose

Fluorescent reporter for circadian rhythms

Depositor

Mary Teruel

Article

Zhang et al Proc Natl Acad Sci U S A. 2022 Aug 16;11

Insert

Reverba-mScarlet (Nr1d1 Mouse)

Expression

Mammalian

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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T7-VEE-mCherry

Plasmid

#193355

Purpose

Self-replicating RNA vector expressing mCherry fluorescent protein + PuroR

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

mCherry

Use

Venezuelan equine encephalitis (vee) virus rna re…

Expression

Mammalian

Promoter

T7

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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EF-hGPC3

Plasmid

#24670

Depositor

Jorge Filmus

Article

Gonzalez et al J Cell Biol. 1998 Jun 15. 141(6):1407-14

Insert

GPC3 (GPC3 Human)

Tags

HA

Expression

Mammalian

Available Since

May 6, 2010

Availability

Academic Institutions and Nonprofits only
TFAM-SNAPf

Plasmid

#187909

Purpose

Mammalian cell expression of SNAPf-labeled TFAM

Depositor

Erika Holzbaur

Article

Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

Insert

TFAM (TFAM Human)

Tags

SNAPf

Expression

Mammalian

Promoter

CMV

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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TRE-UBC-HLA-G SCT-T2A-GFP

Plasmid

#205462

Purpose

lentiviral plasmid for expression of HLA-G SCT

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

HLA-G SCT (HLA-G Human)

Expression

Mammalian

Promoter

UBC

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV/L7-6-GFP-WPRE

Plasmid

#126462

Purpose

An AAV vector that expresses GFP under the control of the L7-6 promoter. The L7-6 promoter is the short size (0.8-kb) and has highly specificity to Purkinje cells.

Depositor

Hirokazu Hirai

Article

Nitta et al Mol Ther Methods Clin Dev. 2017 Jul 27;6

Insert

L7-6, Purkinje cell specific promoter

Use

AAV

Expression

Mammalian

Available Since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

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