165,803 results

1. CoV2-Spike-D614G

   Plasmid

   #177960

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation)

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation) (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D614G

   Promoter

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. lenti-sgRNA-BSD

   Plasmid

   #175583

   Purpose

   Expresses sgRNA for CasRx in mammalian cell

   Depositor

   Qi Xie

   Article

   Xia et al Nucleic Acids Res. 2021 Jul 21;49(13):73

   Insert

   sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMXs-c-Myc

   Plasmid

   #13375

   Purpose

   Retroviral expression of mouse c-Myc

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

   Insert

   myelocytomatosis oncogene (Myc Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available Since

   Jan. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. FTO-dCas9

   Plasmid

   #134781

   Purpose

   Expression fusion protein FTO-dCas9 in Mammalian cells

   Depositor

   Shu-Bing Qian

   Article

   Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

   Insert

   FTO and inactive dCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-eCas9-GFP-U6-gRNA

   Plasmid

   #79145

   Purpose

   All-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9 expression in human pluripotent stem cells

   Depositor

   Jizhong Zou

   Insert

   eSpCas9(1.1)
   

   Use

   CRISPR

   Tags

   2A-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTOX3

   Plasmid

   #127448

   Purpose

   Encodes Tse2 toxin. CAM-R

   Depositor

   Matthew Waldor

   Article

   Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

   Insert

   tse2 toxin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pRha

   Available Since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lys-eMagB-iRFP

   Plasmid

   #162249

   Purpose

   Blue-light dependent heterodimerization system

   Depositor

   Pietro De Camilli

   Article

   Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

   Insert

   p18-eMagB-iRFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   beta-actin promoter

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. qTAG-C-mStayGold-Puro

   Plasmid

   #227253

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-2A-Puro cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-FAH-rtTA3G

   Plasmid

   #120309

   Purpose

   An AAV vector that expresses rtTA3G under the c-fos promoter

   Depositor

   Bong-Kiun Kaang

   Article

   Choi et al Science. 2018 Apr 27;360(6387):430-435.

   Insert

   rtTA3G
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   c-fos

   Available Since

   July 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmCherry-C1_SEPT9 i1

    Plasmid

    #71622

    Purpose

    mammalian expression of human SEPT9 with an mCherry fusion

    Depositor

    Elias Spiliotis

    Article

    Dolat et al J Cell Biol. 2014 Oct 27;207(2):225-35.

    Insert

    Septin 9 (SEPTIN9 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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