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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,211 results for: genome

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Showing: 11721 - 11740 of 16211 results

  4. pYLCRISPR/Cas9P35S-H

    Plasmid
    #66189
    Purpose
    expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), hygro selection
    Depositor
    Yao-Guang Liu
    Article
    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(
    Insert
    Cas9
    Expression
    Plant
    Mutation
    plant-codon optimized with higher GC contents (62…
    Promoter
    cauliflower mosaic virus 35S promoter (P35S)
    Available Since
    June 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    66189-plasmid-map-sequence-id-211913

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  5. pRS416-dCas9-Mxi1 + TetR + pRPR1(TetO)-NotI-gRNA

    Plasmid
    #73796
    Purpose
    pRS416 Ura marked Cen/Ars plasmid with dCas9-Mxi1 under Tef1 promoter, and tet-incucibile RPR1 promoter with NotI cloning site adjacent to gRNA
    Depositor
    Ronald Davis
    Article
    Smith et al Genome Biol. 2016 Mar 8;17(1):45. doi: 1
    Inserts
    dCas9-Mxi1
    Tet Repressor
    Structural gRNA for S pyogenes
    Use
    CRISPR
    Tags
    Mxi1 and NLS
    Expression
    Yeast
    Mutation
    D10A, H840A
    Promoter
    pGPM1, pRPR1(TetO), and pTef1
    Available Since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    73796-plasmid-map-sequence-id-237125

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  6. pOT_5 - lenti-EFS-FMC6.3-28z-2A-puro-2A-tNGFR

    Plasmid
    #181974
    Purpose
    Expresses anti-CD19 CAR (with CD28 signaling domain) linked to puromycin resistance via P2A and to human truncated NGFR via T2A for lentiviral delivery.
    Depositor
    Neville Sanjana
    Article
    Legut et al Nature. 2022 Mar;603(7902):728-735. doi:
    Insert
    FMC6.3-28z CAR; tNGFR (NGFR Human, Synthetic)
    Use
    Lentiviral
    Mutation
    NGFR truncation: aa 11-276
    Available Since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    181974-plasmid-map-sequence-id-355578

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  7. pWZL-Neo-Myr-Flag-DEST

    Plasmid
    #15300
    Purpose
    a Gateway-compatible retroviral destination vector which adds a myristoylation sequence and a FLAG tag to each introduced ORF
    Depositor
    Jean Zhao
    Article
    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    Flag and Myr
    Expression
    Mammalian
    Available Since
    July 12, 2007
    Availability
    Academic Institutions and Nonprofits only
    15300-plasmid-map-sequence-id-190527

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  8. pYPQ233-STU

    Plasmid
    #138111
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized, catalytically deactivated LbCas12a-SRDX repressor without promoter and terminator
    Depositor
    Yiping Qi
    Article
    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:
    Insert
    dLbCas12a-SRDX
    Use
    CRISPR; Gateway compatible cas12a entry clone
    Tags
    NLS
    Expression
    Plant
    Available Since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    138111-plasmid-map-sequence-id-272043

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  10. pOT_6 - lenti-EFS-FMC6.3-BBz-2A-puro-2A-tNGFR

    Plasmid
    #181975
    Purpose
    Expresses anti-CD19 CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A and to human truncated NGFR via T2A for lentiviral delivery.
    Depositor
    Neville Sanjana
    Article
    Legut et al Nature. 2022 Mar;603(7902):728-735. doi:
    Insert
    FMC6.3-BBz CAR; tNGFR (NGFR Human, Synthetic)
    Use
    Lentiviral
    Mutation
    NGFR truncation: aa 11-276
    Available Since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    181975-plasmid-map-sequence-id-377682

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  12. pBFC0619

    Plasmid
    #186690
    Purpose
    VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, GmR+sfGFP cargo
    Depositor
    Jennifer Doudna
    Article
    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:
    Inserts
    tnsA
    tnsB
    tnsC
    tnsD
    tniQ
    cas8-cas5 fusion
    cas7
    cas6
    Vc_2xBsaI_NT (Guide Stuffer) crRNA
    Tn7 transposon
    GmR+sfGFP VcDART Cargo
    Expression
    Bacterial
    Promoter
    PLac
    Available Since
    Sept. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    186690-plasmid-map-sequence-id-373180

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  13. PX458-3xHA-en31FnCas9

    Plasmid
    #201949
    Purpose
    Mammalian expression plasmid of enhanced activity FnCas9 variant en31FnCas9 with T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Debojyoti Chakraborty
    Article
    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:
    Insert
    3xHA-NLS-en31FnCas9-T2A-EGFP
    Use
    CRISPR
    Tags
    3xHA, NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    G1243T, E1369R, E1603H on FnCas9
    Promoter
    Cbh
    Available Since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    201949-plasmid-map-sequence-id-405303

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  14. pYLCRISPR/Cas9P35S-N

    Plasmid
    #66191
    Purpose
    expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), Neo selection
    Depositor
    Yao-Guang Liu
    Article
    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(
    Insert
    Cas9
    Expression
    Plant
    Mutation
    plant-codon optimized with higher GC contents (62…
    Promoter
    cauliflower mosaic virus 35S promoter (P35S)
    Available Since
    Oct. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    66191-plasmid-map-sequence-id-404047

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Showing: 11721 - 11740 of 16211 results