We narrowed to 40,512 results for: Sele

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pZY102

Plasmid

#73933

Purpose

This plasmid was derived from pZY101 by inserting a GUS-intron reporter gene cassette into multiple cloning sites. This plasmid is recommended for use as empty control for service event request.

Depositor

Zhanyuan Zhang

Article

Zeng et al Plant Cell Rep. 2004 Feb;22(7):478-82. E

Inserts

bar
GUS

Expression

Plant

Mutation

intron is inserted into the GUS coding region

Promoter

35S

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pYZ173

Plasmid

#98410

Purpose

Cas9-gRNA lys9 plasmid for lys9 deletion in S. pombe

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Insert

gRNA targeting Sp.lys9 (SPBC3B8.03 Fission Yeast)

Use

CRISPR

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pQTEV-FLJ30046

Plasmid

#34828

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

FLJ30046 (SLAIN1 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pEF HaloTag-SYB2

Plasmid

#197282

Purpose

Encodes full-length Synaptobrevin 2 (SYB2) with mutations Q76V and F77W to confer resistance to tetanus cleavage.

Depositor

Edwin Chapman

Article

Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

Insert

SYB2 and HaloTag (Vamp2 Rat)

Expression

Mammalian

Mutation

Q76V and F77W

Promoter

EF1alpha

Available Since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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mOtop3_pGEMHE

Plasmid

#114676

Purpose

This construct was used to express mouse Otop3 in Xenopus laevis oocytes

Depositor

Emily Liman

Article

Tu et al Science. 2018 Mar 2;359(6379):1047-1050.

Insert

Otopetrin 3 (Otop3 Mouse)

Use

For generating the mrna, which can highly express…

Promoter

T7

Available Since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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pMC0x_gRNA_Pos5_Ptet

Plasmid

#179336

Purpose

gRNA Position Vector 5, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 cam

Depositor

Anke Becker

Article

Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

Insert

Ptet gRNA with sfGFP dropout

Use

CRISPR

Available Since

March 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pMpGWB300

Plasmid

#68628

Purpose

MCS-containing binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Expression

Plant

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGWB204

Plasmid

#68595

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

GUS

Expression

Plant

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-DDK

Plasmid

#137764

Purpose

Control plasmid for immunoprecipitation of DDK/FLAG-tagged proteins. Also a good control for transfection efficiency.

Depositor

Carol Mercer

Article

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

GFP

Tags

DDK

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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Teto Ngn1

Plasmid

#62222

Purpose

Inducible expression of Ngn1

Depositor

Kristin Baldwin

Article

Blanchard et al Nat Neurosci. 2015 Jan;18(1):25-35. doi:

Insert

Neurog1 (Neurog1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

March 5, 2015

Availability

Academic Institutions and Nonprofits only

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pMpGWB302

Plasmid

#68630

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGWB113

Plasmid

#68567

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Glucocorticoid receptor (GR)

Expression

Plant

Promoter

MpEF1alpha promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGWB333

Plasmid

#68661

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Citrine

Expression

Plant

Promoter

MpHSP17.8A1 promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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GST-NDP52

Plasmid

#171422

Purpose

For the purifcation of NDP52 protein

Depositor

James Hurley

Article

Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

Insert

NDP52 (CALCOCO2 Human)

Expression

Bacterial

Available Since

July 15, 2021

Availability

Academic Institutions and Nonprofits only

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p-mCherry-N1-ChloC

Plasmid

#85461

Purpose

Historical plasmid. For inhibition of neuronal activity, #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)

Depositor

Peter Hegemann

Article

Wietek et al Science. 2014 Mar 27.

Insert

ChloC

Tags

mCherry

Expression

Mammalian

Mutation

E90R, T159C

Promoter

CMV (+enhancer)

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pSiMPlc_N + pSiMPlc_C

Plasmid

#134312

Purpose

Maintenance of 2 plasmids with a single antibiotic in E. coli

Depositor

Barbara Di Ventura

Article

Palanisamy et al Nat Commun. 2019 Oct 31;10(1):4967. doi:

Insert

cmR_gp41-1 N-intein + cmR_gp41-1 C-intein

Expression

Bacterial

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-E1.1-RFP

Plasmid

#22928

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

E1.1 binding site from Scardigli et al., 2003 with minimal CMV

Use

AAV

Expression

Mammalian

Available Since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pMpGWB133

Plasmid

#68587

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Citrine

Expression

Plant

Promoter

MpHSP17.8A1 promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pQTEV-PPP1R1A

Plasmid

#31331

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

protein phosphatase 1 (PPP1R1AP1 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pMpGWB134

Plasmid

#68588

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Glucocorticoid receptor (GR)

Expression

Plant

Promoter

MpHSP17.8A1 promoter

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMpGWB334

Plasmid

#68662

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Glucocorticoid receptor (GR)

Expression

Plant

Promoter

MpHSP17.8A1 promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pQTEV-CIP29

Plasmid

#34822

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

cytokine induced protein 29 kDa (SARNP Human)

Tags

His and TEV

Expression

Bacterial

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_P0526-sgRNAmreB

Plasmid

#149654

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi mreB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAmreB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, P0526

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
mOtop2_pGEMHE

Plasmid

#114675

Purpose

This construct was used to express mouse Otop2 in Xenopus laevis oocytes

Depositor

Emily Liman

Article

Tu et al Science. 2018 Mar 2;359(6379):1047-1050.

Insert

Otopetrin 2 (Otop2 Mouse)

Use

For generating the mrna, which can highly express…

Promoter

T7

Available Since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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YSBE 508 : pNEO_GFP

Plasmid

#92081

Purpose

Plasmid for tagging proteins in Cryptococcus neoformans; GFP tag, Neo selection

Depositor

Yong-Sun Bahn

Article

So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

Insert

GFP

Use

tagging proteins in cryptococcus neoforman

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pQTEV-SNX3

Plasmid

#31573

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

sorting nexin 3 (SNX3 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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mOtop2_pcDNA3

Plasmid

#114678

Purpose

This construct was used to express mouse Otop2 in HEK-293 cells

Depositor

Emily Liman

Article

Tu et al Science. 2018 Mar 2;359(6379):1047-1050.

Insert

Otopetrin 2 (Otop2 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pI3-MTU-DO-G418

Plasmid

#160023

Purpose

Integrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). G418 selection.

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMpGWB308

Plasmid

#68636

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Citrine

Expression

Plant

Promoter

MpEF1alpha promoter

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBabe hygro NFS1

Plasmid

#102977

Purpose

Expression of NFS1

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

NFS1 (NFS1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

Silent mutations to eliminate shNFS1_1 binding si…

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
NavBp

Plasmid

#100014

Purpose

Electrophysiology in mammalian cells: Mammalian expression of a voltage gated ion channel and GFP

Depositor

David Clapham, Paul DeCaen

Article

DeCaen et al Elife. 2014 Nov 11;3. doi: 10.7554/eLife

Insert

NavBp

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pYZ146

Plasmid

#98406

Purpose

Cas9-gRNA leu1 plasmid for leu1 deletion in S. pombe

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Insert

gRNA targeting Sp.leu1 (leu1 Fission Yeast)

Use

CRISPR

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pQTEV-PDAP1

Plasmid

#31586

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

PDGFA associated protein 1 (PDAP1 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pMpGWB229

Plasmid

#68620

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

tdTomato

Expression

Plant

Available Since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMpGWB202

Plasmid

#68593

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMpGWB208

Plasmid

#68599

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Citrine

Expression

Plant

Promoter

MpEF1alpha promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pYZ164

Plasmid

#98408

Purpose

Cas9-gRNA his3 plasmid for his3 deletion in S. pombe

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Insert

gRNA targeting Sp.his3 (his3 Fission Yeast)

Use

CRISPR

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pStr-KDEL_APV-SBP-DsRed2-ER-5

Plasmid

#170675

Purpose

Expresses ER-targeted streptavidin-KDEL (hook) and SBP-DsRed2 targeted to the ER lumen with an N-terminal APV tripeptide (cargo) for RUSH.

Depositor

Ke Xu

Article

Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

Inserts

APV-SBP-DsRed2-ER-5
streptavidin-KDEL

Expression

Mammalian

Promoter

IRES and T7

Available Since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMpGWB336

Plasmid

#68664

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

TagRFP

Expression

Plant

Promoter

MpEF1alpha promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSyn-Chronos-mT-Sapphire

Plasmid

#149690

Purpose

For packaging and expression of Chronos-mT-Sapphire in neurons via AAV

Depositor

Robert Malenka

Article

Christoffel et al Proc Natl Acad Sci U S A. 2021 Jun 15;11

Insert

Chronos

Use

AAV

Tags

mT-Sapphire

Expression

Mammalian

Promoter

hSyn

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

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