Addgene

We narrowed to 42,842 results for: Sele

Showing: 2321 - 2360 of 42842 results

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SAH0098-scrPX

Plasmid

#79830

Purpose

Transcriptional interference assessment; no interfering promoter (scrambled)

Depositor

Katja Arndt

Article

Hoffmann et al Nucleic Acids Res. 2016 Jun 2;44(10):e95

Inserts

Blue fluorescent protein
mCherry

Use

Tags

Expression

Mutation

Promoter

bla P3 and scrPX (no promoter activity)

Available since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pFA6a-pcr1P-natMX6-pcr2P

Plasmid

#80620

Purpose

to delete part of the mat1-P locus and fix the mating type in natural S. pombe isolates

Depositor

Miguel Godinho Ferreira

Article

Avelar et al Nat Commun. 2013;4:2235. doi: 10.1038/nc

Inserts

PCR1 from mat1-P
PCR2 from mat1-P

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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pFA6a-pcr1M-natMX6-pcr2M

Plasmid

#80621

Purpose

to delete part of the mat1-M locus and fix the mating type in natural S. pombe isolates

Depositor

Miguel Godinho Ferreira

Article

Avelar et al Nat Commun. 2013;4:2235. doi: 10.1038/nc

Inserts

PCR1 from mat1-M
PCR2 from mat1-M

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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pRL439

Plasmid

#70258

Purpose

Source of psbA promoter of Amaranthus hybridus, used as a strong promoter of cyanobacterial genes

Depositor

Peter Wolk

Article

Elhai et al Gene. 1988 Aug 15;68(1):119-38.

Insert

psbA promoter

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pET11a+T214V

Plasmid

#66134

Purpose

Expresses the T214V mutant of CYP119

Depositor

Teruyuki Nagamune

Article

Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

Insert

CYP119 (cyp119 )

Use

Tags

Expression

Bacterial

Mutation

T214V

Promoter

T7 promoter

Available since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pASKt15C+SrtA2woH

Plasmid

#65018

Purpose

Expresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

Insert

sortase A

Use

Tags

S tag

Expression

Bacterial

Mutation

deleted amino acids 1-59, E105K, E108A

Promoter

tetracycline promoter/operator

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pQTEV-CGI12

Plasmid

#34800

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

CGI-12 (MTERF3 Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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pQTEV-SUHW4

Plasmid

#34807

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

suppressor of hairy wing homolog 4 (Drosophila) (ZNF280D Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pQStrep2-UBL3

Plasmid

#31330

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

ubiquitin-like 3 (UBL3 Human)

Use

Tags

His, StrepII-tag, and TEV

Expression

Bacterial

Mutation

Promoter

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pQTEV-DKFZP566E144

Plasmid

#31593

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

small fragment nuclease (REXO2 Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pNH094

Plasmid

#42167

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

mTFP1-RT-::[(G4S)3]::C-TAP Tag

Use

Tags

Expression

Mutation

Promoter

Available since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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pNH090

Plasmid

#42166

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

mTFP1::[(G4S)3]::C-TAP Tag

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pQTEV-DGCR6

Plasmid

#31564

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

DiGeorge syndrome critical region gene 6 (DGCR6 Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pTNS3

Plasmid

#63127

Purpose

Tn7 transposase expression

Depositor

Herbert Schweizer

Article

Choi et al Appl Environ Microbiol. 2008 Feb;74(4):1

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

P1 and Ptac

Available since

May 17, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pCS2+8CeGFP

Plasmid

#34952

Depositor

Amro Hamdoun

Article

Gokirmak et al J Biol Chem. 2012 Nov 2.

Type

Empty backbone

Use

Sea urchin, zebrafish, xenopus

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

SP6

Available since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only

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HO-Poly-KanMX4-HO

Plasmid

#51662

Purpose

yeast plasmid for integration of target sequence at HO locus, Kan selection

Depositor

David Stillman

Article

Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

Type

Empty backbone

Use

Yeast integration

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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pSPble

Plasmid

#63561

Purpose

Bleomycin drug selection marker

Depositor

Greg Matlashewski

Article

Zhang et al MBio. 2015 Jul 21;6(4). pii: e00861-15.

Insert

Bleomycin drug selection marker

Use

Leishmania expression

Tags

Expression

Mutation

Promoter

Available since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pTW358f

Plasmid

#227935

Purpose

Express hIAPP-T7n

Depositor

Tina Wang

Article

Yang et al Nat Chem Biol. 2025 Jan 13. doi: 10.1038

Insert

hIAPP-T7n

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pbacPuroR

Plasmid

#26348

Depositor

Ben Lehner

Article

Semple et al Nat Methods. 2012 Jan 30;9(2):118-9. doi

Insert

Bacterial promoter::PuroR::bacterial 3p flank

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 3, 2010

Availability

Academic Institutions and Nonprofits only

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pNIC-GST-TEV-GG

Plasmid

#215795

Purpose

Cloning of target gene with N terminal GST tag and TEV cleavage

Depositor

ASAP Discovery, Frank von Delft

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNIC-CT-sfGFP-HN-GG

Plasmid

#215803

Purpose

Cloning of target gene with C terminal TEV cleavable sfGFP HisAsn6 tag

Depositor

ASAP Discovery, Frank von Delft

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pLOVE empty vector

Plasmid

#15948

Depositor

Miguel Ramalho-Santos

Article

Blelloch et al Cell Stem Cell. 2007 Sep 13. ():.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 7, 2007

Availability

Academic Institutions and Nonprofits only

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pNIC-CTH10STII-GG

Plasmid

#215804

Purpose

Cloning of target gene with C terminal TEV cleavage of His10 and Twin Strep tag

Depositor

ASAP Discovery, Frank von Delft

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCS2+8CmCherry

Plasmid

#34935

Depositor

Amro Hamdoun

Article

Gokirmak et al J Biol Chem. 2012 Nov 2.

Type

Empty backbone

Use

Sea urchin, zebrafish, xenopus

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

SP6

Available since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pNX3b2-FL3

Plasmid

#78388

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xFLAG
hygML

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pNXRVb-HACre

Plasmid

#78401

Purpose

constitutive Cre expression vector

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xHA-nls-Cre
hygMX6

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pCS2+8NeGFP

Plasmid

#34953

Depositor

Amro Hamdoun

Article

Gokirmak et al J Biol Chem. 2012 Nov 2.

Type

Empty backbone

Use

Sea urchin, zebrafish, xenopus

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

SP6

Available since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+8NmCherry

Plasmid

#34936

Depositor

Amro Hamdoun

Article

Gokirmak et al J Biol Chem. 2012 Nov 2.

Type

Empty backbone

Use

Sea urchin, zebrafish, xenopus

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

SP6

Available since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only
pNKY51

Plasmid

#14839

Depositor

Nancy Kleckner

Article

Alani et al Genetics. 1987 Aug . 116(4):541-5.

Insert

hisG-URA3-hisG

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 27, 2007

Availability

Academic Institutions and Nonprofits only
pGRG37

Plasmid

#16667

Depositor

Nancy Craig

Article

McKenzie et al BMC Microbiol. 2006 . 6():39.

Insert

mTn7::Gateway cassette

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only

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pPuroG1

Plasmid

#200224

Purpose

puroR nls-mNG marked rapid RMCE integration vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

Type

Empty backbone

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pNIC-MBP-TEV-GG

Plasmid

#215794

Purpose

Cloning of target gene with N terminal MBP tag and TEV cleavage

Depositor

ASAP Discovery, Frank von Delft

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pJQ200

Plasmid

#78496

Purpose

Base vector for construction of series of suicide plasmids for gene replacement in Proteobacteria

Depositor

Michael Hynes

Article

Quandt et al Gene. 1993 May 15;127(1):15-21.

Type

Empty backbone

Use

Suicide vector

Tags

Expression

Mutation

Promoter

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pNIC-HIS6-SUMO1-GG

Plasmid

#215799

Purpose

Cloning of target gene with N terminal His6 SUMO2 tag and SENP1 cleavage

Depositor

ASAP Discovery, Frank von Delft

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCS2+8CCerulean

Plasmid

#34937

Depositor

Amro Hamdoun

Article

Gokirmak et al J Biol Chem. 2012 Nov 2.

Type

Empty backbone

Use

Sea urchin, zebrafish, xenopus

Tags

Cerulean

Expression

Mammalian

Mutation

Promoter

SP6

Available since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only
pNX3c-HA3

Plasmid

#78373

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xHA
natMX6

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-Flightless-1-LRR-HA

Plasmid

#21151

Depositor

Junying Yuan

Article

Li et al J Cell Biol. 2008 Apr 21. 181(2):321-33.

Insert

pcDNA3-Fli-Lrr-HA

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2009

Availability

Academic Institutions and Nonprofits only
pCS2+8CmCitrine

Plasmid

#34950

Depositor

Amro Hamdoun

Article

Gokirmak et al J Biol Chem. 2012 Nov 2.

Type

Empty backbone

Use

Sea urchin, zebrafish, xenopus

Tags

mCitrine

Expression

Mammalian

Mutation

Promoter

SP6

Available since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only
pCS2+8NCerulean

Plasmid

#34938

Depositor

Amro Hamdoun

Article

Gokirmak et al J Biol Chem. 2012 Nov 2.

Type

Empty backbone

Use

Sea urchin, zebrafish, xenopus

Tags

Cerulean

Expression

Mammalian

Mutation

Promoter

SP6

Available since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only
pBS34

Plasmid

#83796

Purpose

Useful for tagging yeast proteins with C-terminal mCherry tag; G418 selection

Depositor

Eric Muller

Article

Hailey et al Methods Enzymol. 2002;351:34-49.

Insert

mCherry

Use

Tags

mCherry

Expression

Yeast

Mutation

Promoter

Available since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 2321 - 2360 of 42842 results

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