We narrowed to 76,996 results for: rest

1. pKLV-U6gRNA(BbsI)-PGKpuro2ABFP-L1mono3

   Plasmid

   #73542

   Purpose

   Expression of sgRNA targeting LINE-1 and TagBFP

   Depositor

   Constance Ciaudo

   Article

   Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

   Insert

   LINE-1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKLV-U6gRNA(BbsI)-PGKpuro2ABFP-L1mono1

   Plasmid

   #73543

   Purpose

   Expression of sgRNA targeting LINE-1 and TagBFP

   Depositor

   Constance Ciaudo

   Article

   Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

   Insert

   LINE-1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. L1-AA027

   Plasmid

   #185752

   Purpose

   Level 1 loop assembly vector containing a transcriptional unit with a Hygromycin resistance cassette under the control of the A. agrestis EF1a promoter (pAaEF1a).

   Depositor

   Peter Szoevenyi

   Article

   Frangedakis et al New Phytol. 2021 Jun 2. doi: 10.1111/nph

   Insert

   HygR2
   

   Use

   Synthetic Biology

   Expression

   Plant

   Available Since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Prham-M.XhoI-M.MunI-M.Ocy1ORF8430P-p15A-aadA

   Plasmid

   #170768

   Purpose

   Methylase expression vector for 5RM assembly: expresses M.XhoI, M.MunI (MfeI) and M.Ocy1ORF8430P (SpeI) DNA methyltransferases in E. coli

   Depositor

   Ichiro Matsumura

   Article

   Matsumura et al Biotechniques. 2022 May;72(5):185-193. d

   Inserts

   M.XhoI
   M.MunI
   M.Ocy1ORF8430P
   

   Expression

   Bacterial

   Promoter

   rhamnose

   Available Since

   Feb. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIIS18-BufAI-2

   Plasmid

   #128651

   Purpose

   Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

   Depositor

   Tohru Suzuki

   Article

   Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

   Type

   Empty backbone

   Use

   Unspecified

   Available Since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIIS18-BufAI-1

   Plasmid

   #128650

   Purpose

   Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

   Depositor

   Tohru Suzuki

   Article

   Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

   Type

   Empty backbone

   Use

   Unspecified

   Available Since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTK-EdnrbE2-Cerulean

   Plasmid

   #127771

   Purpose

   Enhancer reporter construct containing the chicken EdnrB enhancer, E2 driving Cerulean activity

   Depositor

   Tatjana Sauka-Spengler

   Article

   Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

   Insert

   ednrbE2
   

   Use

   Enhancer reporter construct

   Available Since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pU6-Sox3gRNA-T2

   Plasmid

   #127778

   Purpose

   Chicken sox3 gRNA target site 2, cloned into the pU6 sgRNA (backbone #92359)

   Depositor

   Tatjana Sauka-Spengler

   Article

   Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

   Insert

   sox3gRNAT2
   

   Use

   CRISPR

   Available Since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX 4T-1/MKRN1

   Plasmid

   #78757

   Purpose

   To purify GST-tagged MKRN1 recombinant protein

   Depositor

   Jaewhan Song

   Article

   Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

   Insert

   MKRN1 (MKRN1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   June 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-HA-MKRN1 110-482

    Plasmid

    #78747

    Purpose

    To overexpress MKRN1 110-482 in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

    Insert

    MKRN1 (MKRN1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    110-482

    Promoter

    CMV

    Available Since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-HA-MKRN1 1-280

    Plasmid

    #78750

    Purpose

    To overexpress -MKRN1 1-280 in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

    Insert

    MKRN1 (MKRN1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    1-280

    Promoter

    CMV

    Available Since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBGSA FPR1 N297A

    Plasmid

    #66723

    Purpose

    Constitutive expression of FPR1 N297A in mammalian cells.

    Depositor

    Heini Miettinen

    Article

    Gripentrog et al Cell Signal. 2008 Feb;20(2):424-31. Epub

    Insert

    FPR1 (haplotype 3) (FPR1 Human)
    

    Expression

    Mammalian

    Mutation

    N297A

    Promoter

    SR-alpha

    Available Since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pCMV-myc-Rem2 S334A

    Plasmid

    #51593

    Purpose

    expresses RNAiR myc-tagged Rem2 S-A mutant

    Depositor

    Suzanne Paradis

    Article

    Ghiretti et al J Neurosci. 2013 Apr 10;33(15):6504-15.

    Insert

    Rem2 (Rem2 Mouse)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Serine 334 to Alanine; also RNAi resistant

    Promoter

    CMV

    Available Since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pUC:FCP:ShBle:FCP:EGFP

    Plasmid

    #85987

    Purpose

    Expresses ShBle and EGFP with FCP promoters/terminators for F. cylindrus transformation.

    Depositor

    Thomas Mock

    Article

    Faktorova et al Nat Methods. 2020 May;17(5):481-494. doi

    Inserts

    ShBle
    EGFP
    

    Promoter

    FCP

    Available Since

    Jan. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSIV-D3psi/delta env/delta Vif/delta Vpr

    Plasmid

    #132928

    Purpose

    Production of SIV-VLPs containing Vpx protein

    Depositor

    Jeremy Luban

    Article

    Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

    Insert

    Gag, pol, and vpx
    

    Use

    Lentiviral

    Available Since

    Oct. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFA6a-kanMX6-P41nmt1-mECitrine

    Plasmid

    #105152

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu

    Article

    Wang et al J Cell Biol. 2014 May 12;205(3):357-75.

    Insert

    kanMX6-P41nmt1-mECitrine
    

    Tags

    41nmt1-mECitrine

    Expression

    Bacterial and Yeast

    Mutation

    F64L, Q69M, A206K

    Promoter

    41nmt1

    Available Since

    Feb. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGL3b-8XGliBS:EGFP

    Plasmid

    #84602

    Purpose

    Gli protein-dependent EGFP reporter for analysis of Hedgehog signaling

    Depositor

    James Chen

    Article

    Hyman et al Proc Natl Acad Sci U S A. 2009 Aug 18;10

    Insert

    eGFP
    

    Expression

    Mammalian

    Promoter

    8X Gli-binding site

    Available Since

    Oct. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. ∆DUSP6

    Plasmid

    #27977

    Depositor

    Igor Astsaturov

    Article

    Bagnyukova et al Br J Cancer. 2013 Aug 20;109(4):1063-71.

    Insert

    DUSP6 (DUSP6 Human)
    

    Use

    Lentiviral

    Tags

    HA and MYC

    Expression

    Mammalian

    Mutation

    C293S: mutation in the phosphatase domain

    Available Since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pLenti_CamKIIa_GFP

    Plasmid

    #96941

    Purpose

    To label mature excitatory iPSC-derived human neurons

    Depositor

    Ricardo Dolmetsch, Oleksandr Shcheglovitov

    Article

    Shcheglovitov et al Nature. 2013 Nov 14;503(7475):267-71. do

    Insert

    Green fluorescent protein
    

    Use

    Lentiviral

    Promoter

    CaMKIIa

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX459-HypaCas9-mR2-AAVS1_sgRNA

    Plasmid

    #183890

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and sgRNA targeting the AAVS1 in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    AAVS1 sgRNA spacer (AAVS1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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