We narrowed to 19,528 results for: MUT

Showing: 11821 - 11840 of 19528 results

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pcDNA3-FRB-LplA(W37I)

Plasmid

#34840

Depositor

Alice Ting

Article

Slavoff et al J Am Chem Soc. 2011 Dec 14;133(49):19769

Insert

FRB-LplA(W37I)

Tags

FLAG (internal fusion), HA, and His Tag

Expression

Mammalian

Mutation

LplA has Trp37 to Val mutation

Promoter

CMV

Available Since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pReporter_10

Plasmid

#60570

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_10

Expression

Bacterial

Promoter

BBa_J23119

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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Flag-TRIM24 shRescue

Plasmid

#28139

Depositor

Michelle Barton

Article

Allton et al Proc Natl Acad Sci U S A. 2009 Jul 14. 1

Insert

TRIM24 (TRIM24 Human)

Tags

FLAGX2

Expression

Mammalian

Mutation

A489V; aa 308 and 309 mutated for shRNA rescue

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
MBP-TEV-VMA Model vector

Plasmid

#86590

Purpose

This can be used to make MBP-TEV-Cterm with SapI and PstI; and can also make N-term-VMA with NdeI and SapI

Depositor

Sharon Rozovsky

Article

Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

Insert

VMA intein

Tags

His6-MBP

Expression

Bacterial

Mutation

Original SapI site from pMal was mutated out

Promoter

Tac

Available Since

Feb. 24, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pCAGGS_2paCRY2(D387A)

Plasmid

#129652

Purpose

2P-activatable CRY2 with the FAD-disrupting mutation

Depositor

Michiyuki Matsuda

Article

Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

Insert

2paCRY2(D387A)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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M-R742X (BF7-2)

Plasmid

#21372

Depositor

Gregory Germino

Article

Hanaoka et al Nature. . 408(6815):990-4.

Insert

autosomal dominant polycystic kidney disease type II (PKD2 Human)

Tags

6xMyc

Expression

Mammalian

Mutation

changed R742 to stop codon

Available Since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only
pSec7-Halox2

Plasmid

#115425

Purpose

Express Sec7-Halox2 at the endogenous locus via pop-in pop-out mutagenesis

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

Insert

Sec7-Halox2

Tags

HaloTag

Expression

Yeast

Promoter

Endogenous

Available Since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt6-V5

Plasmid

#35932

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt6 (WNT6 Human)

Tags

V5

Expression

Mammalian

Mutation

Silent Mutation G (567) --> A

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt9A-V5

Plasmid

#35937

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt9A (WNT9A Human)

Tags

V5

Expression

Mammalian

Mutation

Silent Mutation A (543) --> G

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pTU1-A-lacZ

Plasmid

#72935

Purpose

Level 1 Destination vector - Position A

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Level 1 - A site

Use

Synthetic Biology

Mutation

Beta-lactamase - G717C (nucleotide) - silent muta…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pICH42222

Plasmid

#50328

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, phosphinothricin acetyl transferase, (S. hygroscopicus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation; See De…

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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YIplac211-vps10-104

Plasmid

#115426

Purpose

Generate the vps10-104 allele via pop-in pop-out mutagenesis

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

Insert

vps10-104

Expression

Yeast

Mutation

domain 1 deletion

Promoter

Endogenous

Available Since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA HA FAK Y577F

Plasmid

#50507

Depositor

Kenneth Yamada

Article

Tamura et al J Biol Chem. 1999 Jul 16;274(29):20693-7

Insert

protein tyrosine kinase, mutated

Tags

HA

Expression

Mammalian

Mutation

Y577F

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pICSL70005

Plasmid

#50335

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

bialaphos resistance cassette (Nos promoter (A. tumefaciens) + phosphinothricin acetyl transferase (S. hygroscopicus) + Nos term

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pED7x26

Plasmid

#134813

Purpose

Stringent phage propagation reporter containing pIII-16-29-34-TAGA

Depositor

Ahmed Badran

Article

DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

Insert

pIII 16-TAGA 29-TAGA 34-TAGA

Use

Synthetic Biology

Expression

Bacterial

Mutation

16-TAGA 29-TAGA 34-TAGA

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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UbG75C

Plasmid

#37194

Depositor

Cynthia Wolberger

Article

Wiener et al Nature. 2012 Feb 22;483(7391):618-22. do

Insert

Ubiquitin G75C mutant (RBX1 Human)

Tags

No tag

Expression

Bacterial

Mutation

Glycine 75 changed to Cystine

Promoter

T7

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only
YIp211-P30-His-pol30(K164R)-3'

Plasmid

#99548

Purpose

Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K164R; URA3 marker

Depositor

Helle Ulrich

Article

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

POL30 (POL30 Budding Yeast)

Tags

6xHis

Expression

Yeast

Mutation

K164R

Promoter

POL30

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR-L5-CAG-R3

Plasmid

#141036

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

CAG promoter

Expression

Mammalian

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAC-EsaR-I70V

Plasmid

#47663

Purpose

pAC-EsaR with a208g mutation in esaR

Depositor

Cynthia Collins

Article

Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

Insert

esaR-I70V

Use

Synthetic Biology

Expression

Bacterial

Mutation

Changed Isoleucine 70 to Valine

Promoter

Plac

Available Since

Sept. 6, 2013

Availability

Academic Institutions and Nonprofits only
pDH6

Plasmid

#83777

Purpose

Useful for tagging yeast proteins with C-terminal YFP tag; G418 selection

Depositor

Eric Muller

Article

Hailey et al Methods Enzymol. 2002;351:34-49.

Insert

YFP

Tags

YFP

Expression

Yeast

Mutation

S65G, V68L, Q69K, S72A, T203Y mutations added.

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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