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pUCmini-iCAP-PHP.V1

Plasmid

#127847

Purpose

non-standard AAV2 rep-AAV-PHP.V1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Publication

Ravindra Kumar et al Nat Methods. 2020 May;17(5):541-550. doi

Insert

Synthetic construct isolate AAV-PHP.V1 VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pUCmini-iCAP-PHP.V1

Plasmid

#127847

Purpose

non-standard AAV2 rep-AAV-PHP.V1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Publication

Ravindra Kumar et al Nat Methods. 2020 May;17(5):541-550. doi

Insert

Synthetic construct isolate AAV-PHP.V1 VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pET_RTX-6xHis

Plasmid

#145029

Purpose

pET vector for IPTG-inducible, T7-based expression of RTX bearing a C-terminal 6xHis tag

Depositor

Andrew Ellington

Publication

Bhadra et al BioProtocol 2021

Insert

RTX

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pET_RTX-6xHis

Plasmid

#145029

Purpose

pET vector for IPTG-inducible, T7-based expression of RTX bearing a C-terminal 6xHis tag

Depositor

Andrew Ellington

Publication

Bhadra et al BioProtocol 2021

Insert

RTX

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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SaKKH-ABE8e

Plasmid

#138502

Purpose

A-to-G base editor

Depositor

David Liu

Publication

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(8e)-nSaCas9 (KKH)

Expression

Mammalian

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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SaKKH-ABE8e

Plasmid

#138502

Purpose

A-to-G base editor

Depositor

David Liu

Publication

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(8e)-nSaCas9 (KKH)

Expression

Mammalian

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pLJC5-3XMyc-EGFP-PEX26

Plasmid

#139059

Purpose

Myc-PEROXO Lentiviral Construct. Expresses a Peroxisomal Tag.

Depositor

David Sabatini

Publication

Ray et al iScience. 2020 May 22;23(5):101109. doi:

Insert

3XMyc-EGFP-PEX26

Use

Lentiviral

Tags

Myc

Promoter

UBC

Available since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pLJC5-3XMyc-EGFP-PEX26

Plasmid

#139059

Purpose

Myc-PEROXO Lentiviral Construct. Expresses a Peroxisomal Tag.

Depositor

David Sabatini

Publication

Ray et al iScience. 2020 May 22;23(5):101109. doi:

Insert

3XMyc-EGFP-PEX26

Use

Lentiviral

Tags

Myc

Promoter

UBC

Available since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCRISPR-cBEST

Plasmid

#125689

Purpose

C to T base editor for actinomycetes

Depositor

Tilmann Weber

Publication

Tong et al Proc Natl Acad Sci U S A. 2019 Oct 8;116

Insert

streptomyces codon optimized spCas9n (D10A), modified sgRNA casette, streptomyces codon optimized rAPOBEC1

Use

CRISPR and Synthetic Biology

Promoter

ermE*/tipA

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pCRISPR-cBEST

Plasmid

#125689

Purpose

C to T base editor for actinomycetes

Depositor

Tilmann Weber

Publication

Tong et al Proc Natl Acad Sci U S A. 2019 Oct 8;116

Insert

streptomyces codon optimized spCas9n (D10A), modified sgRNA casette, streptomyces codon optimized rAPOBEC1

Use

CRISPR and Synthetic Biology

Promoter

ermE*/tipA

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pRN114

Plasmid

#127221

Purpose

BeYDV replicon with constitutive Luc expression, WUS2 and AtSTM

Depositor

Daniel Voytas

Publication

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc, Wus, AtSTM

Expression

Plant

Mutation

codon optimized CDSs

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pRN114

Plasmid

#127221

Purpose

BeYDV replicon with constitutive Luc expression, WUS2 and AtSTM

Depositor

Daniel Voytas

Publication

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc, Wus, AtSTM

Expression

Plant

Mutation

codon optimized CDSs

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-6XHis-human SUMO 2

Plasmid

#133771

Purpose

express 6Xhis- SUMO 2 protein

Depositor

Martine Roussel

Publication

Tago et al Proc Natl Acad Sci U S A. 2005 May 24. 1

Insert

SUMO 2 (SUMO2 Human)

Tags

6XHis

Expression

Mammalian

Promoter

CMV

Available since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3-6XHis-human SUMO 2

Plasmid

#133771

Purpose

express 6Xhis- SUMO 2 protein

Depositor

Martine Roussel

Publication

Tago et al Proc Natl Acad Sci U S A. 2005 May 24. 1

Insert

SUMO 2 (SUMO2 Human)

Tags

6XHis

Expression

Mammalian

Promoter

CMV

Available since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLX401-INK4A

Plasmid

#121919

Purpose

Doxycycline-inducible expression of p16-INK4A product of CDKN2A

Depositor

William Hahn

Publication

Howard et al Cancer Res. 2019 Feb 12. pii: 0008-5472.

Insert

p16-INK4A (CDKN2A Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE Tet ON

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pLX401-INK4A

Plasmid

#121919

Purpose

Doxycycline-inducible expression of p16-INK4A product of CDKN2A

Depositor

William Hahn

Publication

Howard et al Cancer Res. 2019 Feb 12. pii: 0008-5472.

Insert

p16-INK4A (CDKN2A Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE Tet ON

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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mNeonGreen-mRuby2-FRET-10

Plasmid

#58179

Purpose

Localization: Cytosolic FRET, Excitation: , Emission:

Depositor

Michael Davidson

Publication

Shaner et al Nat Methods. 2013 May;10(5):407-9. doi:

Type

Empty backbone

Tags

mNeonGreen and mRuby2

Expression

Mammalian

Available since

March 1, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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mNeonGreen-mRuby2-FRET-10

Plasmid

#58179

Purpose

Localization: Cytosolic FRET, Excitation: , Emission:

Depositor

Michael Davidson

Publication

Shaner et al Nat Methods. 2013 May;10(5):407-9. doi:

Type

Empty backbone

Tags

mNeonGreen and mRuby2

Expression

Mammalian

Available since

March 1, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Bassik lab Mouse CRISPR-Cas9 Deletion Library - Drug targets, kinases, phosphatases

Pooled library

#1000000122

Purpose

CRISPR gRNA mouse knockout pooled library - Drug targets, kinases, phosphatases

Depositor

Michael Bassik

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-ZNF598-TEV-3xFLAG

Plasmid

#105690

Purpose

Expresses C terminally 3xFLAG tagged ZNF598 protein

Depositor

Ramanujan Hegde

Publication

Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

Insert

ZNF598 (ZNF598 Human)

Tags

TEV-3xFLAG

Expression

Mammalian

Promoter

CMV (from vector)

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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