We narrowed to 14,544 results for: TIM

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pUDE1093

Plasmid

#204227

Purpose

Episomal yeast plasmid expressing the Ercas12a

Depositor

Jean-Marc Daran

Article

Bennis et al FEMS Yeast Res. 2023 Jan 4;23:foad043. d

Insert

Eubacterium rectale cas12a (Ercas12a)

Expression

Yeast

Mutation

codon optimized for S. cerevisiae

Promoter

Saccharomyces cerevisiae PGK1

Available Since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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LLP784_SnoopCatcher-DNMT3A-CO

Plasmid

#211777

Purpose

SnoopTag counterpart binding domain, SnoopCatcher, fused to DNA methyltransferase DNMT3A, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

Spy-EZH2

Tags

3xFLAG

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect EZH2 e…

Promoter

pEF1a and pSV40

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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BAT-gal

Plasmid

#20889

Depositor

Stefano Piccolo

Article

Maretto et al Proc Natl Acad Sci U S A. 2003 Mar 18. 1

Insert

7XTCF binding sites, Siamois minimal promoter, NLS-lacz

Use

Mouse Targeting

Expression

Mammalian

Mutation

7 multimerized TCF-binding elements cloned upstre…

Available Since

May 7, 2009

Availability

Academic Institutions and Nonprofits only
FgH1tUTG_huBim_Ex3

Plasmid

#85532

Purpose

Inducible expression of guide RNA (huBim_Ex3) with fluorescent GFP reporter

Depositor

Marco Herold

Article

Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

Insert

hu Bim

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

H1t

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pRGEB-VirD2-Cas9

Plasmid

#139803

Purpose

Expressing VirD-Cas9 fusion under ubiquitin promoter

Depositor

Magdy Mahfouz

Article

Ali et al Commun Biol. 2020 Jan 23;3(1):44. doi: 1

Insert

VirD2-Cas9

Tags

VirD2-Cas9

Expression

Plant

Promoter

Ubiquitin

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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KM-CashGem-Xyl2-URA3-LINEAR

Plasmid

#174838

Purpose

pRS414 backbone containing LINEAR fragment targeting XYL2 in K. marxianus

Depositor

Zengyi Shao

Article

Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

Insert

Codon optimized Cas9:hGem

Use

CRISPR and Synthetic Biology

Tags

hGeminin tag

Expression

Bacterial and Yeast

Promoter

TEF1p

Available Since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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LLP787_SnoopCatcher-EZH2-FL-CO

Plasmid

#211780

Purpose

SnoopTag counterpart binding domain, SnoopCatcher, fused to polycomb repressive complex subunit EZH2, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

aGCN4-DNMT3A

Tags

3xTy1

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect DNMT3A…

Promoter

pEF1a and pSV40

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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LLP780_SpyCatcher-KRAB-CO

Plasmid

#211773

Purpose

SpyTag counterpart binding domain, SpyCatcher, fused to transcriptional repressor KRAB, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

SpyCatcher-KRAB

Tags

3xFLAG

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect KRAB e…

Promoter

pEF1a and pSV40

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pMXs_FLAG-MCART6

Plasmid

#133251

Purpose

Express FLAG-tagged MCART6 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

Insert

MCART6 (SLC25A53 Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

codon-optimized for expression in human cells

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCXLE-dCas9VPPH-T2A-GFP-shP53

Plasmid

#102897

Purpose

EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-P300core-p65-HSF1 followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

dCas9VPPH-T2A-GFP-shP53

Use

CRISPR

Expression

Mammalian

Mutation

D10A, H840A

Promoter

CAG

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pEJS1004-pCSDest2-AcrIIA5-NLS-FLAG

Plasmid

#136513

Purpose

Expresses type II-A anti-CRISPR protein AcrIIA5 in mammalian cells

Depositor

Erik Sontheimer

Article

Garcia et al Cell Rep. 2019 Nov 12;29(7):1739-1746.e5

Insert

codon-optimized AcrIIA5

Tags

FLAG/NLS

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

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pRSETa-mEos3.2-A69T

Plasmid

#98571

Purpose

photoconvertible fluorescent protein mEos3.2-A69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coli

Depositor

Ulrike Endesfelder

Article

Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

Insert

mEos3.2-A69T

Tags

7xHis

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2018

Availability

Academic Institutions and Nonprofits only

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plenti hSyn PGK1-HALO

Plasmid

#220910

Purpose

Neuronal specific expression of the fusion construct PGK1-HALO

Depositor

Timothy Ryan

Article

Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

Insert

PGK1-HALO (PGK1 Human)

Use

Lentiviral

Tags

HaloTag

Expression

Mammalian

Promoter

hSyn

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Hygro(-)-B2-TS3

Plasmid

#104353

Purpose

Expresses human beta 2 integrin tension sensor

Depositor

Pontus Nordenfelt, Timothy Springer

Article

Nordenfelt et al Nat Commun. 2016 Oct 10;7:13119. doi: 10

Insert

Human integrin b2 with tension sensor (ITGB2 Human)

Expression

Mammalian

Mutation

AA1-733 ITGB2 present

Promoter

CMV

Available Since

Jan. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pTH41 [punc-17::YFP-BeCyclOp(A-2x)::SL2::mCherry]

Plasmid

#168170

Purpose

Expression of YFP-BeCyclOp(A-2x) in cholinergic motor neurons of C. elegans

Depositor

Alexander Gottschalk

Article

Henss et al Br J Pharmacol. 2021 Mar 18. doi: 10.111

Insert

YFP-BeCyclOp(A-2x) (codon optimized for C. elegans)

Tags

YFP

Expression

Worm

Mutation

E497K and C566D

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pHS1477

Plasmid

#205970

Purpose

MWEE01000013 Cas5-HNH operon (E. coli codon optimized) with His14-tagged Cse2 in pET45b(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas5-HNH all proteins, with His-tagged Cas11

Tags

14xHis on Cas11

Expression

Bacterial

Promoter

T7

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pESC-URA-hPOLISc

Plasmid

#60819

Purpose

to express human Pol iota recoded to yeast codons in yeast

Depositor

Youri Pavlov

Article

Makarova et al PLoS One. 2011 Jan 31;6(1):e16612. doi:

Insert

POLI (POLI Human)

Tags

c-myc

Expression

Yeast

Mutation

optimized to yeast codon usage

Promoter

GAL1

Available Since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA-CRY2-mCh-superPLDx30-P2A-CIBN-CAAX

Plasmid

#188992

Purpose

A plasmid for dual expression of CRY2-mCh-superPLDx30 (an engineered PLD mutant with 30 times higher activity than the wild-type) and plasma membrane-tagged CIBN

Depositor

Jeremy Baskin

Article

Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

Insert

PLD

Tags

CAAX, CIBN, CRY2, P2A, and mCherry

Expression

Mammalian

Mutation

K109R, P245A, G328S, G381V, G429D

Promoter

CMV

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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LITE1.0_pAAV_hSyn_CRY2PHR-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA

Plasmid

#47454

Purpose

epiLITE / LITE1.0 CRY2PHR fused to SID4X transcriptional repressor effector domain. Binds to CIB1 upon blue light stimulation. Synapsin promoter for neuronal expression.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

Insert

CRY2PHR-NLS-SID4X

Use

AAV

Tags

2A_phiLOV2.1

Expression

Mammalian

Promoter

hSyn human synapsin (mammalian neuronal expressio…

Available Since

Oct. 15, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HCMVgL-FLAG (Merlin)

Plasmid

#136450

Purpose

Mammalian expression plasmid for HCMV glycoprotein L (Merlin strain) with FLAG tag

Depositor

Erik Procko

Article

Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

Insert

glycoprotein L (UL115 Human betaherpesvirus 5)

Tags

Short SGGSG linker and FLAG tag

Expression

Mammalian

Mutation

Codon-optimized for human cell expression.

Promoter

CMV

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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