We narrowed to 44,431 results for: gats

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pU6_(GLuc)_INT[Spinach2]

Plasmid

#68430

Purpose

Transient expression in mammalian cells of an "INT" construct_bearing the "Spinach2" aptamer, targeting the GLuc reporter. U6 promoter

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

INT construct_bearing the "Spinach2" aptamer

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

human U6

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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mApple-ERM

Plasmid

#217377

Purpose

ER marker

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

rat cb5 ERM

Tags

mApple

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pSub-MBP-GFP(2M-IKQE)

Plasmid

#185757

Purpose

Bacterial expression of MBP-GFP(2M IKQE)

Depositor

Jeffrey Bode

Article

Akimoto et al ACS Cent Sci. 2022 Feb 23;8(2):275-281.

Insert

GFP(2M-IKQE)

Tags

MBP

Expression

Bacterial

Promoter

tac

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pET29b-mVenus-dspB(E184Q)-6xHis

Plasmid

#176576

Purpose

Plasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

Depositor

Mark Nitz

Article

Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

Insert

Dispersin B

Tags

Hexahistidine tag and mVenus

Expression

Bacterial

Mutation

E184Q

Promoter

T7

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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plIVMD156

Plasmid

#160934

Purpose

In vivo mRNA display Gateway Destination construct

Depositor

Saeed Tavazoie

Article

Oikonomou et al Proc Natl Acad Sci U S A. 2020 Oct 9. pi

Insert

MCP-Gateway cloning site-HIS tag, 3'UTR MS2 Stem Loop

Tags

HIS tag, 3'UTR MS2 Stem Loop

Expression

Yeast

Promoter

MET25

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pUE1-TSP-ORF68

Plasmid

#162650

Purpose

Expresses ORF68 in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Tags

N-terminal 2xStrep-Tag II and HRV 3C site

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pJW4.16

Plasmid

#62871

Purpose

Bacterial expression of MBP-MBK-2

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

MBP-MBK-2

Tags

MBP

Expression

Bacterial

Available Since

April 22, 2015

Availability

Academic Institutions and Nonprofits only

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MECP2 - R133C in pENTR

Plasmid

#216594

Purpose

gateway cloning donor vector containing MECP2 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

MECP2 (MECP2 Human)

Use

Gateway entry plasmid

Mutation

R133C

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pTORKm43GWC

Plasmid

#112898

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418). Contains additional cassette for constitutive expression of mTFP1.

Depositor

Sebastian Schornack

Article

Evangelisti et al MBio. 2019 Oct 1;10(5). pii: mBio.01516-

Type

Empty backbone

Use

Oomycete expression

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV syn CCR6-eLOV-TEVcs-tTA

Plasmid

#173116

Purpose

Expresses CCR6 receiver construct in neurons

Depositor

Alice Ting

Article

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

CCR6-eLOV-TEVcs-tTA

Use

AAV

Promoter

Synapsin

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pET22b-T5-FB-E25TAG

Plasmid

#176545

Purpose

Expresses IgG affinity protein FB with a TAG stop codon at E25 position

Depositor

Han Xiao

Article

Yu et al Bioconjug Chem. 2018 Nov 21;29(11):3522-

Insert

IgG affinity protein

Tags

His tag

Expression

Bacterial

Mutation

change glutamic acid 25 to amber stop codon

Promoter

T5

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR macroH2A1.2-C

Plasmid

#30512

Depositor

John Gurdon

Article

Pasque et al EMBO J. 2011 May 6;30(12):2373-87. doi:

Insert

macroH2A1.2-C (Macroh2a1 Mouse)

Use

Gateway - donor vector

Mutation

NO STOP

Available Since

June 25, 2012

Availability

Academic Institutions and Nonprofits only

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SLF091: Lenti-CaMKIIa-eTsChR-eGFP

Plasmid

#112281

Purpose

For lentiviral or direct expression of eTsChR in excitatory neurons

Depositor

Adam Cohen

Article

Farhi et al J Neurosci. 2019 Apr 5. pii: JNEUROSCI.0

Insert

eTsChR

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CaMKIIa

Available Since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQ-NF-L1-dMb2Cas12a

Plasmid

#177683

Purpose

Gateway entry clone (attL1 & attR5) for dimeric FokI-dMb2Cas12a genome editing

Depositor

Yiping Qi

Article

Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

Insert

FokI-dMb2Cas12a

Use

CRISPR; Gateway compatible foki-dmb2cas12a entry …

Tags

NLS

Expression

Plant

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pRU52

Plasmid

#167678

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

PcUB4-2::spCas9

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCRISPR_eGFP_369

Plasmid

#111823

Purpose

Knockout eGFP

Depositor

Steve Shih

Article

Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

Insert

gRNA targeting eGFP 349-369

Use

CRISPR

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

U6

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pDB036a

Plasmid

#99215

Purpose

Accessory plasmid for PACE of MjTyrRS variants; negative selection

Depositor

David Liu

Article

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); PproD [SD4] T7RNAP(S12*,S203*)

Expression

Bacterial

Mutation

amber codons at positions 12 and 203 of T7 RNA po…

Promoter

Ppsp, PproK and PproD

Available Since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pASKt15C+SrtA5

Plasmid

#64985

Purpose

Expresses the P94R/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

Insert

sortase A

Tags

His6 tag and S tag

Expression

Bacterial

Mutation

deleted amino acids 1-59, P94R, D160N, D165A, K19…

Promoter

tetracycline promoter/operator

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pTH760-CEN-mCherry_v3

Plasmid

#40598

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Insert

mCherry (Red Fluorescent Protein variant) with yeast promoter and terminator sequences

Expression

Yeast

Mutation

mCherry ORF partially codon optimised for yeast e…

Promoter

GPD (TDH3)

Available Since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pMpGWB100

Plasmid

#68062

Purpose

MCS-containing binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Expression

Plant

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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