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pDONR223_POLR2I_WT_V5

Plasmid

#82955

Purpose

Gateway Donor vector containing POLR2I, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

POLR2I (POLR2I Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-IGLON5-COMP5AP-AviTag-9xHis

Plasmid

#157307

Purpose

Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

IGLON5 (IGLON5 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3R1-K379E

Plasmid

#116596

Purpose

Lentiviral expression of PIK3R1 K379E

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3R1 (PIK3R1 Human)

Use

Lentiviral

Mutation

K379E

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-KIR3DP1-Fc(DAPA)-AviTag-6xHis

Plasmid

#156746

Purpose

Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

KIR3DP1 (KIR3DP1 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CNTF-HA

Plasmid

#195517

Purpose

Expresses HA-tagged CNTF in mammalian cells

Depositor

Kevin Park

Article

Ribeiro et al Exp Neurol. 2022 Sep;355:114147. doi: 10

Insert

Ciliary neurotrophic factor (CNTF Human)

Use

AAV

Tags

HA-tag and NGF signal peptide

Expression

Mammalian

Promoter

UbC promoter with beta-globin intron

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-TNFRSF11B-Fc(DAPA)-AviTag-6xHis

Plasmid

#156811

Purpose

Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

TNFRSF11B (TNFRSF11B Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLEX307_PDHA2S293A

Plasmid

#115197

Purpose

Lentiviral transduction and expression of PDHA2S293A into any mammalian cell

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

PDHA2S293A (PDHA2 Human)

Use

Lentiviral

Mutation

p.S293A

Promoter

EF1alpha

Available Since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA216-CoV2-RBD-beta variant

Plasmid

#194598

Purpose

Mammalian expression of RBD domain from SARS-CoV-2 beta (B.1.351)

Depositor

Ben Murrell

Article

Sheward et al Cell Rep Med. 2021 Nov 16;2(11):100450.

Insert

CoV2-RBD-beta variant (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223_RBM45_WT_V5

Plasmid

#82968

Purpose

Gateway Donor vector containing RBM45, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

RBM45 (RBM45 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_STC2_WT_V5

Plasmid

#82957

Purpose

Gateway Donor vector containing STC2, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

STC2 (STC2 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE-MAN2B1

Plasmid

#116756

Purpose

Lentiviral expression of MAN2B1

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

MAN2B1 (MAN2B1 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5 FLAG membrane-bound lipin-1 (TMD)

Plasmid

#120277

Purpose

Expression of ER/Golgi-tethered lipin-1 in mammalian cells

Depositor

Sirio Dupont

Article

Romani et al Nat Cell Biol. 2019 Mar;21(3):338-347. d

Insert

Lipin-1 (Lpin1 Mouse)

Tags

FLAG and in-frame fusion with the TMD Golgi-local…

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CD-C416R

Plasmid

#116564

Purpose

Lentiviral expression of PIK3CD C416R

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CD (PIK3CD Human)

Use

Lentiviral

Mutation

C416R

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CB-K290N

Plasmid

#116556

Purpose

Lentiviral expression of PIK3CB K290N

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CB (PIK3CB Human)

Use

Lentiviral

Mutation

K290N

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CB-R321Q

Plasmid

#116559

Purpose

Lentiviral expression of PIK3CB R321Q

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CB (PIK3CB Human)

Use

Lentiviral

Mutation

R321Q

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CB-A1048V

Plasmid

#116550

Purpose

Lentiviral expression of PIK3CB A1048V

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CB (PIK3CB Human)

Use

Lentiviral

Mutation

A1048V

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PTEN-R130Q

Plasmid

#116631

Purpose

Lentiviral expression of PTEN R130Q

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PTEN (PTEN Human)

Use

Lentiviral

Mutation

R130Q

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PPP2R1A-V525A

Plasmid

#116609

Purpose

Lentiviral expression of PPP2R1A V525A

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PPP2R1A (PPP2R1A Human)

Use

Lentiviral

Mutation

V525A

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3R1-Q455_K459delQFQEK

Plasmid

#116602

Purpose

Lentiviral expression of PIK3R1 Q455_K459delQFQEK

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3R1 (PIK3R1 Human)

Use

Lentiviral

Mutation

Q455_K459delQFQEK

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3R1-W333R

Plasmid

#116606

Purpose

Lentiviral expression of PIK3R1 W333R

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3R1 (PIK3R1 Human)

Use

Lentiviral

Mutation

W333R

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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