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LentiGuide-Neo-CTRLg2

Plasmid

#139451

Purpose

Lentiviral vector with non-targeting gRNA and neomycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

Non-targeting sgRNA inserted; resistance gene: neoR

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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FUW-ubiquitin-SV40-RFP

Plasmid

#65448

Purpose

Expression of RFP in mammalian cells. Control vector for FUW-Ephrin-RFP vectors

Depositor

Eduard Batlle

Article

Cortina et al Nat Genet. 2007 Nov;39(11):1376-83. Epub

Insert

SV40-RFP

Use

Lentiviral

Expression

Mammalian

Promoter

UBQ

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
HOXA10-MSCV

Plasmid

#20980

Depositor

Corey Largman

Article

Thorsteinsdottir et al Mol Cell Biol. 1997 Jan . 17(1):495-505.

Insert

HOXA10 (HOXA10 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
Cav2.2e[a10, 18a, Δ24a, 31a, 37b, 46]

Plasmid

#26568

Depositor

Diane Lipscombe

Article

Pan et al J Neurosci. 2000 Jul 1. 20(13):4769-75.

Insert

calcium channel alpha-1B subunit (Cacna1b Rat)

Tags

6XHis and V5

Expression

Mammalian

Mutation

contains exons a10, 18a, 31a, 37b, and 46. Del…

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only
LentiGuide-Puro-CTRLg1 (CG509)

Plasmid

#139455

Purpose

Lentiviral vector with non-targeting gRNA and puromycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

Non-targeting sgRNA inserted; resistance gene: puroR

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pHDR_NANOS3-T2A-mVenus-PuroTK

Plasmid

#222903

Purpose

Homology directed repair template for knocking in mVenus reporter to NANOS3.

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

mVenus

Use

CRISPR

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416 Gal G294A YFP

Plasmid

#27448

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Tags

YFP

Expression

Yeast

Mutation

Glycine 294 mutated to Alanine (G294A)

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only
SARS_COV2_orf9c_HA_BASU

Plasmid

#153991

Purpose

Express Orf9c gene along with HA and BASU

Depositor

Paul Khavari

Article

Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

Insert

orf9c

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shFXN_1

Plasmid

#102970

Purpose

Suppression of FXN

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shFXN_1 (FXN Human)

Use

Lentiviral and RNAi

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3 PBD wt (Nigg AH12)

Plasmid

#41162

Depositor

Erich Nigg

Article

Hanisch et al Mol Biol Cell. 2006 Jan;17(1):448-59. Ep

Insert

PBD (PLK1 Human)

Tags

3xMyc and PreScission Site

Expression

Mammalian

Mutation

Contains Plk1-PBD only (aa 326–603)

Promoter

CMV

Available Since

June 7, 2013

Availability

Academic Institutions and Nonprofits only
p4xSMAD_RE

Plasmid

#118051

Purpose

GBPart - Operator/DNA Response element - 4 copies the SMAD DNA binding motif

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

4 copies the SMAD DNA binding motif

Use

Synthetic Biology; Domestication of dna parts for…

Expression

Mammalian

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pBluescript-mouse eIF2C2

Plasmid

#50354

Purpose

Mouse eIF2C2 cDNA clone

Depositor

Kumiko Ui-Tei

Article

Doi et al Curr Biol. 2003 Jan 8;13(1):41-6.

Insert

eIF2C2 (Ago2 Mouse)

Expression

Bacterial

Promoter

T7

Available Since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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p2xp53_RE

Plasmid

#118053

Purpose

GBPart - Operator/DNA Response element - 2 copies of the p53 DNA binding motif

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

2 copies of the p53 DNA binding motif

Use

Synthetic Biology; Domestication of dna parts for…

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) M-Ras(WT)

Plasmid

#224287

Purpose

Cell transfection and expression of M-Ras

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

M-Ras (MRAS Human)

Expression

Mammalian

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pHS0667 KraIscB-1 non-coding locus in pCOLADuet-1

Plasmid

#176537

Purpose

Non-protein coding locus components from KraIscB-1

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Insert

non-coding locus components from KraIscB-1 locus

Expression

Bacterial

Available Since

Nov. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV-SMYD2 F184A

Plasmid

#85659

Purpose

retroviral expression of human SMYD2 F184A

Depositor

Julien Sage

Article

Reynoird et al Genes Dev. 2016 Apr 1;30(7):772-85. doi:

Insert

SMYD2 (SMYD2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

F184A

Promoter

LTR

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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GST-Bcr pLEF

Plasmid

#38160

Depositor

Nora Heisterkamp

Article

Mishra et al Oncogene. 2003 Nov 13;22(51):8255-62.

Insert

Bcr (BCR Human)

Tags

GST

Expression

Mammalian

Promoter

SV40

Available Since

Aug. 3, 2012

Availability

Academic Institutions and Nonprofits only
Ad-Flag-HDAC3

Plasmid

#63675

Purpose

adenoviral expression of Flag tagged HDAC3

Depositor

Qingbo Xu, Lingfang Zeng

Article

Zeng et al J Cell Biol. 2006 Sep 25;174(7):1059-69.

Insert

HDAC3 (Hdac3 Mouse)

Use

Adenoviral

Tags

FLAG

Expression

Mammalian

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only
pECE-M2-PAK2 S20A

Plasmid

#31681

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

p21-activated protein kinase 2 S20A (PAK2 Human)

Tags

M2

Expression

Mammalian

Mutation

changed Serine 20 to Alanine

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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phFluc-WT

Plasmid

#177714

Purpose

Cytosolic firefly luciferase reporter previously described in Shcherbakov et al. 2019

Depositor

Erik Böttger

Article

Shcherbakov et al Commun Biol. 2019 Oct 17;2:381. doi: 10.

Insert

hFluc WT

Expression

Bacterial and Mammalian

Available Since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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