We narrowed to 11,284 results for: 110

1. pRSET-4xTR-TUBE

   Plasmid

   #110312

   Purpose

   E. coli expression and purification of 4xTR-TUBE

   Depositor

   Yasushi Saeki

   Article

   Tsuchiya et al Nat Commun. 2018 Feb 6;9(1):524. doi: 10

   Insert

   4xTR-TUBE (UBQLN1 Human)
   

   Use

   Tags

   N-terminal His6-T7 tag

   Expression

   Bacterial

   Mutation

   All Arg residues in the UBA domain are mutated to…

   Promoter

   T7

   Available since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKL

   Plasmid

   #110741

   Purpose

   Plasmid for the insect cell expression system, acceptor for plasmid fusions, two promoters, Multiplication Module M, Tn7 transposition and LoxP site

   Depositor

   Tim Richmond

   Article

   Fitzgerald et al Nat Methods. 2006 Dec;3(12):1021-32. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin and p10 promoters

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. Hy_pMT Flag-URH49 WT

   Plasmid

   #110127

   Purpose

   Expresses Flag-tagged human URH49 (DDX39A)

   Depositor

   Jeremy Wilusz

   Article

   Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

   Insert

   URH49 (DDX39A Human)
   

   Use

   Tags

   Flag

   Expression

   Insect

   Mutation

   Promoter

   Metallothionein Promoter (pMT)

   Available since

   April 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKDM

   Plasmid

   #110740

   Purpose

   Plasmid for the insect cell expression system, two promoters, Multiplication Module M, Tn7 transposition

   Depositor

   Tim Richmond

   Article

   Fitzgerald et al Nat Methods. 2006 Dec;3(12):1021-32. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-TRE-TdTomato

   Plasmid

   #110338

   Purpose

   TdTomato reporter vector of iTango and Cal-Light system for AAV production

   Depositor

   Hyungbae Kwon

   Insert

   tetO-TdTomato
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tetO

   Available since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMEV4

   Plasmid

   #110539

   Purpose

   The plasmid serves as a backbone for heterologous expression in the model archaeal organism Methanococcus maripaludis.

   Depositor

   William B. Whitman

   Article

   Lyu et al ACS Synth Biol. 2016 Jul 15;5(7):577-81.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 28, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pCDNA3-Flag-SPRTN-E112A

   Plasmid

   #110215

   Purpose

   Expression in mammalian cells of of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, and Flag-tag at N-terminus.

   Depositor

   Ivana Marinovic-Terzic, Kristijan Ramadan

   Article

   Lessel et al Nat Genet. 2014 Nov;46(11):1239-44. doi:

   Insert

   SPRTN (SPRTN Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   E112A, P296L (see depositor comments below)

   Promoter

   Available since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-N1_MeCP2(WT)

   Plasmid

   #110186

   Purpose

   Expression of mouse MeCP2 (WT) in mammalian cells

   Depositor

   Adrian Bird

   Article

   Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

   Insert

   MeCP2_e2 FL (mouse cDNA) (Mecp2 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   May 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. SB_concave

   Plasmid

   #110102

   Purpose

   pBXPHM3 with scaffold Sybody of the concave library

   Depositor

   Markus Seeger

   Article

   Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

   Insert

   scaffold Sybody of the concave library
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   PBAD

   Available since

   May 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. Human USP30 (1-517, WT, MG-38-11)

    Plasmid

    #110748

    Purpose

    Transient transfection of human USP30

    Depositor

    David Komander

    Article

    Gersch et al Nat Struct Mol Biol. 2017 Sep 25. doi: 1

    Insert

    USP30 (USP30 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. Halo-Tev-Flag-Ube2v2

    Plasmid

    #110070

    Purpose

    Express Halo-Tev-Flag-Ube2v2 (human) in mammalian cells

    Depositor

    Yimon Aye

    Article

    Zhao et al ACS Cent Sci. 2018 Feb 28;4(2):246-259.

    Insert

    Halo-Tev-Flag-Ube2v2 (UBE2V2 Human)
    

    Use

    Tags

    Halo-TEV-Flag

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    July 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pACT5FVMF(f)

    Plasmid

    #110549

    Purpose

    Control plasmid for titering FV by qPCR to determine the genome-containing particle number. This qPCR protocol is described in Hendrie et al., 2008

    Depositor

    David Russell

    Article

    Hendrie et al Mol Ther. 2008 Mar;16(3):534-40. doi: 10

    Insert

    EGFP, FV LTRs
    

    Use

    Qpcr control for titering foamy virus

    Tags

    Expression

    Mutation

    Promoter

    promoter: MLV LTR

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti6.3-OGDH_wobble

    Plasmid

    #110938

    Purpose

    OGDH ORF containing silent wobble mutations insensitive to corresponding shRNAs

    Depositor

    William Hahn

    Article

    Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

    Insert

    OGDH wobble cDNA (OGDH Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    15 silent wobble mutations corresponding to three…

    Promoter

    SV40

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-TRE3G-FNLS-PGK-Puro

    Plasmid

    #110847

    Purpose

    Lentiviral vector for dox-inducible expression of FNLS in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3RA-FNLS
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    D10A and NLS sequence at the N-terminus

    Promoter

    TRE3G

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3 Leo1

    Plasmid

    #11061

    Depositor

    Matthew Meyerson

    Article

    Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

    Insert

    Leo1 (LEO1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3 Paf1

    Plasmid

    #11059

    Depositor

    Matthew Meyerson

    Article

    Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

    Insert

    Paf1 (PAF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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17. pB-CAGGS-dCas9

    Plasmid

    #110823

    Purpose

    PiggyBac compatible plasmid expressing dCas9

    Depositor

    Alejandro Chavez, George Church

    Article

    Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

    Insert

    dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    CAGGS

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSKC11 - UTRQ

    Plasmid

    #110490

    Purpose

    Monitor the expression of Luciferase driven by the NRAS 5'-UTR.

    Depositor

    Shankar Balasubramanian

    Article

    Kumari et al Nat Chem Biol. 2007 Apr;3(4):218-21. doi

    Insert

    NRAS 5'-UTR (bp 1-254) (NRAS Human)
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mutation

    Promoter

    T7

    Available since

    July 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. SB_loop

    Plasmid

    #110103

    Purpose

    pBXPHM3 with scaffold Sybody of the loop library

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

    Insert

    scaffold Sybody of the loop library
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    May 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. mCRY1 HA tag

    Plasmid

    #110298

    Purpose

    full length wt mouse Cry1 HA tag in pcDNA3.1

    Depositor

    Joseph Takahashi

    Article

    Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

    Insert

    wt mcry1 HA tag (Cry1 Mouse)
    

    Use

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    wildtype

    Promoter

    CMV

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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