We narrowed to 165,848 results for: Gene

1. KJ740(DE3)

   Bacterial Strain

   #179486

   Purpose

   KJ740(DE3) is an OmpC-, OmpF-deletion strain of E. coli that allows for T7 promoter-based expression

   Depositor

   Debra Hansen

   Article

   Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

   Bacterial Resistance

   Tetracycline (chromosome, tn10) (10 μg/ml); streptomycin/spectinomycin (chromosome) (50 μg/ml)

   Species

   Escherichia coli

   Available Since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

2. BIOFAB

   Plasmid Kit

   #1000000037

   Purpose

   Sets of specific constitutive bacterial promoters that vary in strength, followed by specific Bi-Cistronic Design elements varying in effectiveness to drive E. coli expression of a gene of interest

   Depositor

   Drew Endy

   Available Since

   Dec. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

3. MK01

   Bacterial Strain

   #195090

   Purpose

   Improved E. coli strain created by knocking out the endogenous lacI gene with chloramphenicol acetyltransferase (cat) selection cassette in the parent strain BW27783 that overcome the all-or-none response of araBAD promoter (PBAD).

   Depositor

   Sander Tans

   Article

   Kogenaru et al J Biol Eng. 2014 Jan 2;8(1):2. doi: 10.1

   Bacterial Resistance

   Chloramphenicol

   Available Since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

4. Guzit

   Bacterial Strain

   #196902

   Purpose

   Guzit is a RNase HI(rnhA) His-tagged E. coli strain made for a cell-free expression system.

   Depositor

   Kate Adamala

   Article

   Sato et al Biotechnol Bioeng. 2023 Jul;120(7):1986-

   Bacterial Resistance

   Kanamycin

   Available Since

   Aug. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

5. RNase III-His

   Bacterial Strain

   #196903

   Purpose

   RNase III-His is an E. coli strain made for a cell-free expression system.

   Depositor

   Kate Adamala

   Article

   Sato et al Biotechnol Bioeng. 2023 Jul;120(7):1986-

   Bacterial Resistance

   Kanamycin

   Available Since

   Aug. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

6. C-5545 ∆cosδε

   Bacterial Strain

   #209018

   Purpose

   Deficient in the P2 bacteriophage packaging cos site and encoding rhamnose-inducible P4 delta (δ) and epsilon (ε) genes

   Depositor

   Alfonso Jaramillo

   Article

   Tridgett et al ACS Synth Biol. 2021 Jan 15;10(1):107-11

   Bacterial Resistance

   Hygromycin

   Species

   Escherichia coli

   Available Since

   Jan. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

7. Strain SIJ_488

   Bacterial Strain

   #68246

   Purpose

   E. coli K-12 MG1655 with genomically integrated arabinose inducible lambda Red recombineering genes, rhamnose inducible flippase recombinase and m-toluic acid inducible I-SceI.

   Depositor

   Alex Nielsen

   Article

   Jensen et al Sci Rep. 2015 Dec 8;5:17874. doi: 10.103

   Bacterial Resistance

   None

   Available Since

   Dec. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

8. Syn61Δ3(ev5)

   Bacterial Strain

   #174514

   Purpose

   Recoded E. coli strain without TCG, TCA, or TAG codons and deleted serT, serU and prfA genes. Full sequence - Genbank: CP071799.1

   Depositor

   Jason W Chin

   Article

   Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

   Bacterial Resistance

   Streptomycin

   Available Since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

9. TP997

   Bacterial Strain

   #13055

   Depositor

   Anthony Poteete

   Article

   Poteete et al BioTechniques. 2006 Sept. 41(3):261-264

   Bacterial Resistance

   Ampicillin and spectinomycin

   Species

   Tn1696

   Available Since

   Sept. 26, 2006

   Availability

   Academic Institutions and Nonprofits only

10. FD1

    Bacterial Strain

    #125258

    Purpose

    Derivative of MG1655 used for the screening of clones able to survive the bad seed toxicity of dCas9, while still efficiently silencing the expression of mcherry

    Depositor

    David Bikard

    Article

    Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)

    Bacterial Resistance

    None

    Available Since

    Aug. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

11. Jun lab tunable CRISPRi strain

    Bacterial Strain

    #86400

    Purpose

    Strain SJ_XTL219 Genotype: MG1655 PBAD_dcas9 ΔlacI ΔaraE araFGH<>spec lacY A177C, galM<pBBa-J23119-tet-sacB-handle-term

    Depositor

    Suckjoon Jun

    Article

    Li et al Sci Rep. 2016 Dec 20;6:39076. doi: 10.10

    Bacterial Resistance

    Spectinomycin and hygromycin

    Available Since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

12. pAAV-TREtight-mTagBFP2-B19G (AAV1)

    Viral Prep

    #100799-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-TREtight-mTagBFP2-B19G (#100799). In addition to the viral particles, you will also receive purified pAAV-TREtight-mTagBFP2-B19G plasmid DNA. Helper virus for monosynaptic tracing with rabies virus; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA (Addgene# 100798). These AAV preparations are suitable purity for injection into animals.

    Depositor

    Ian Wickersham

    Article

    Liu et al Nature. 2017 Aug 31;548(7669):582-587. d

    Promoter

    TREtight

    Tags

    BFP (in presence of Cre and 100798)

    Available Since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

13. Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A (Lentiviral Prep)

    Viral Prep

    #92379-LV

    Purpose

    Ready-to-use Lentiviral Prep particles produced from Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A (#92379). In addition to the viral particles, you will also receive purified Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A plasmid DNA. Ready-to-use lentiviral pooled library for CRISPR activation screening in human cells. Contains 56,762 sgRNAs, targeting 18,885 genes. Concentrated lentiviral particles carrying set A of the Calabrese human CRISPRa sgRNA activation library in backbone XPR_502 (P65 HSF) .

    Depositor

    David Root, John Doench

    Article

    Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

    Promoter

    Tags

    None

    Available Since

    Aug. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

14. RF12

    Bacterial Strain

    #62077

    Purpose

    BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. trpA trpB genes deleted.

    Depositor

    Robert Gennis, Toshio Iwasaki

    Article

    Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

    Bacterial Resistance

    None

    Available Since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

15. zCas9i cloning kit: MoClo-compatible CRISPR/Cas9 cloning kit with intronized Cas9

    Plasmid Kit

    #1000000171

    Purpose

    Vectors and DNA modules for assembly of CRISPR/Cas9 constructs for use in plants. The constructs are made with zCas9i, a high efficiency intron-optimized SpCas9-coding gene.

    Depositor

    Johannes Stuttmann, Sylvestre Marillonnet

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

16. EasyClone-MarkerFree Vector Set

    Plasmid Kit

    #1000000098

    Purpose

    Yeast (S. cerevisiae) vector toolkit for marker-less integration of genes into 11 predetermined EasyClone Chromosomal loci via CRISPR-Cas9

    Depositor

    Irina Borodina

    Available Since

    Dec. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

17. BL21∆serB

    Bacterial Strain

    #34929

    Depositor

    Dieter Söll, Jesse Rinehart

    Article

    Park et al Science. 2011 Aug 26;333(6046):1151-4.

    Bacterial Resistance

    None

    Available Since

    Feb. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

18. FR-E01

    Bacterial Strain

    #118727

    Purpose

    Expression of dCas9 gene under the control of a Ptet promoter in the E. coli chromosome

    Depositor

    David Bikard

    Article

    Rousset et al PLoS Genet. 2018 Nov 7;14(11):e1007749.

    Bacterial Resistance

    None

    Available Since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

19. Top10∆serB

    Bacterial Strain

    #34928

    Depositor

    Dieter Söll, Jesse Rinehart

    Article

    Park et al Science. 2011 Aug 26;333(6046):1151-4.

    Bacterial Resistance

    None

    Available Since

    Feb. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

20. RAS mutant clone collection

    Plasmid Kit

    #1000000089

    Purpose

    Wild-type and mutant HRAS, NRAS, KRAS4a and KRAS4b gene plasmids as Gateway entry clones

    Depositor

    Dominic Esposito

    Available Since

    Dec. 1, 2016

    Availability

    Academic Institutions and Nonprofits only