We narrowed to 22,010 results for: his

1. 6x-His_A1aY1_GFP

   Plasmid

   #158753

   Purpose

   Bacterial expression of 6xHis-A1aY1 fused with carboxy terminus GFP for recombinant purification

   Depositor

   Minhajuddin Sirajuddin

   Article

   Kesarwani et al J Cell Biol. 2020 Oct 5;219(10). pii: 15

   Insert

   6xHistidine_A1aY1_GFP
   

   Use

   Tags

   6x-Histidine tag, GFP, T7 leader sequence, and Th…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHis17_ParM _2

   Plasmid

   #78202

   Purpose

   Expression of double-cysteine variant of plasmid segregation protein, ParM, as scaffold for ADP biosensor

   Depositor

   Martin Webb

   Article

   Kunzelmann et al ACS Chem Biol. 2010 Apr 16;5(4):415-25.

   Insert

   parM (K33A D63C T174A T175N D224C C287A)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   K33A D63C T174A T175N D224C C287A

   Promoter

   unknown

   Available since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFastBacMam5_N_Flag_His8_eGFP_3C

   Plasmid

   #178816

   Purpose

   Baculovirus gene transfer into Mammalian cells to product recombinant proteins.

   Depositor

   Kazuhiro Abe

   Type

   Empty backbone

   Use

   Tags

   Flag, His8, eGFP, 3C

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFastBacMam6_C_3C_His8_Stop

   Plasmid

   #178810

   Purpose

   Baculovirus gene transfer into Mammalian cells to product recombinant proteins.

   Depositor

   Kazuhiro Abe

   Type

   Empty backbone

   Use

   Tags

   3C, His8, Stop codon

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTAMAHISTEV_PfeA

   Plasmid

   #128943

   Purpose

   expresses PfeA in E. coli

   Depositor

   James Naismith

   Article

   Moynie et al Nat Commun. 2019 Aug 14;10(1):3673. doi:

   Insert

   ferric enterobactin receptor
   

   Use

   Tags

   TEV protease cleavable 7xHis and TamA

   Expression

   Bacterial

   Mutation

   Signal peptide sequence has been removed (1-75)

   Promoter

   T7

   Available since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBIG1b_10xHisTEVATG5_ATG12

   Plasmid

   #190861

   Purpose

   Plasmid for the expression of ATG5 and ATG12 protein subunit complexes. Internal References: SMC1178

   Depositor

   Sascha Martens

   Article

   Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

   Inserts

   ATG5 (ATG5 Human)
   ATG12 (ATG12 Human)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTAMAHISTEV_PfeAQ482A

   Plasmid

   #128945

   Purpose

   expresses Q482A mutant of PfeA in E. coli

   Depositor

   James Naismith

   Article

   Moynie et al Nat Commun. 2019 Aug 14;10(1):3673. doi:

   Insert

   ferric enterobactin receptor
   

   Use

   Tags

   TEV protease cleavable 7xHis and TamA

   Expression

   Bacterial

   Mutation

   Signal peptide sequence has been removed (1-75), …

   Promoter

   T7

   Available since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28_Human_SAT1_Cterm_His

   Plasmid

   #220487

   Purpose

   heterologous expression of SSAT1 for protein purification

   Depositor

   Emily Balskus

   Article

   Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

   Insert

   SSAT1 (SAT1 Human)
   

   Use

   Tags

   6xHs

   Expression

   Bacterial

   Mutation

   none

   Promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28_Human_SAT1_Nterm_His

   Plasmid

   #220482

   Purpose

   heterologous expression of SSAT1 for protein purification

   Depositor

   Emily Balskus

   Article

   Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

   Insert

   SSAT1 (SAT1 Human)
   

   Use

   Tags

   6xHs

   Expression

   Bacterial

   Mutation

   none

   Promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a_speG_Cterm_His

    Plasmid

    #220483

    Purpose

    heterologous expression for protein purification

    Depositor

    Emily Balskus

    Article

    Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

    Insert

    speG
    

    Use

    Tags

    6xHs

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGagMBPHis

    Plasmid

    #42176

    Depositor

    James Hurley

    Article

    Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

    Insert

    Gag protein sequence is from HIV-1 clone NL4-3
    

    Use

    Tags

    6xHIS and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pET28a_PA4114_Cterm_His

    Plasmid

    #220484

    Purpose

    heterologous expression of PA4114 for protein purification

    Depositor

    Emily Balskus

    Article

    Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

    Insert

    PA4114
    

    Use

    Tags

    6xHs

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. p2Bac.His.XPB

    Plasmid

    #39216

    Depositor

    Aziz Sancar

    Insert

    XPB (ERCC3 Human)
    

    Use

    Tags

    His

    Expression

    Insect

    Mutation

    Promoter

    Pph

    Available since

    Aug. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

14. p2Bac.His.XPD

    Plasmid

    #39217

    Depositor

    Aziz Sancar

    Insert

    XPD (ERCC2 Human)
    

    Use

    Tags

    His (HHYHHH)

    Expression

    Insect

    Mutation

    XPD starts at aa4

    Promoter

    Pph

    Available since

    Aug. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

15. HisMS2_VLP_FluA

    Plasmid

    #175955

    Purpose

    Generation of MS2 Virus-Like Particles packaged with the sequence for the Influenza A Matrix Protein [A/Illinois/20/2018(H1N1)]

    Depositor

    Paul Freemont

    Article

    Unruh et al medRxiv 2024.04.17.24305965

    Insert

    M gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pMGAHisTev5B

    Plasmid

    #37221

    Depositor

    Jon Lorsch

    Article

    Acker et al Methods Enzymol. 2007;430:111-45.

    Insert

    eIF5B
    

    Use

    Tags

    His

    Expression

    Mutation

    N-term truncation (contains aa 396-1002)

    Promoter

    Available since

    Aug. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pHisIHFαIHFβ

    Plasmid

    #149384

    Purpose

    Expression of P. aeruginosa PA14, 6xHis-HRV3C-IHFalpha and IHFbeta

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Inserts

    Pseudomonas aeruginosa PA14 6xHis-tagged IHFalpha
    Pseudomonas aeruginosa PA14 IHFbeta
    

    Use

    Tags

    6xHis tag and HRV3C cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOPINVHH_cys_his

    Plasmid

    #210403

    Purpose

    For expression of VHH with C-terminal cysteine and his tag

    Depositor

    Ray Owens

    Article

    Eyssen et al Bio Protoc. 2024 Mar 20;14(6):e4962. doi

    Type

    Empty backbone

    Use

    Tags

    cys-his6

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOPINVHH_his_flag

    Plasmid

    #210404

    Purpose

    For expression of VHH with C-terminal his and Flag tags

    Depositor

    Ray Owens

    Article

    Eyssen et al Bio Protoc. 2024 Mar 20;14(6):e4962. doi

    Type

    Empty backbone

    Use

    Tags

    his6-flag

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOPINVHH_sortase_his

    Plasmid

    #210406

    Purpose

    For expression of VHH with C-terminal sortase and his tags

    Depositor

    Ray Owens

    Article

    Eyssen et al Bio Protoc. 2024 Mar 20;14(6):e4962. doi

    Type

    Empty backbone

    Use

    Tags

    sortase-his6

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge