We narrowed to 56,262 results for: plasmid

1. RNA motif plasmid cloning backbone

   Plasmid

   #107253

   Purpose

   RNA motif plasmid with BoxB sites. Digest with BsmBI and insert RNA motif of interest. Stuffer size 1.8kb

   Depositor

   Paul Khavari

   Article

   Ramanathan et al Nat Methods. 2018 Feb 5. pii: nmeth.4601

   Insert

   RNA motif plasmid cloning backbone
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDY153: Flag(SUZ12) WT HDR plasmid

   Plasmid

   #170793

   Purpose

   WT homology directed repair plasmid for performing genome-editing on the SUZ12 Locus

   Depositor

   Thomas Cech

   Article

   Youmans et al Mol Cell. 2021 Feb 4;81(3):488-501.e9. d

   Insert

   SUZ12 (SUZ12 Human)
   

   Expression

   Mammalian

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. ER-mit RspA-splitFAST (lentiviral plasmid)

   Plasmid

   #233604

   Purpose

   Expression of ER-mit RspA-splitFAST by a single lentiviral plasmid encoding the two fragments

   Depositor

   Riccardo Filadi

   Article

   Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

   Insert

   RspA-splitFAST
   

   Use

   Lentiviral

   Promoter

   CMV/SV40

   Available Since

   April 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGHUC w-ERBIN (2-hybrid prey plasmid)

   Plasmid

   #128163

   Purpose

   UV5 promoter drives expression of omega-erbin fusion. Pair with pB1H2 UV5 Zif268-cons as a positive control (turns on HIS3/GFP).

   Depositor

   Marcus Noyes

   Article

   Ichikawa et al ACS Synth Biol. 2019 May 17;8(5):918-928

   Insert

   ERBIN PDZ domain (ERBIN Synthetic)
   

   Use

   Synthetic Biology

   Tags

   FLAG and Omega subunit of RNA polymerase

   Expression

   Bacterial

   Promoter

   UV5

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCDH-CMV-RPS15A-3xHA plasmid

   Plasmid

   #192245

   Purpose

   overexpression vector of RPS15A-3XHA

   Depositor

   Dieter Wolf

   Article

   Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

   Insert

   RPS15A CDS without stop coden (RPS15A Human)
   

   Use

   Lentiviral

   Tags

   3xHA

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSHS207 - Bacterial expression plasmid for WT SpCas9

   Plasmid

   #101199

   Purpose

   Bacterial expression plasmid for WT SpCas9

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9
   

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAGGS-ACE2-his expression plasmid

   Plasmid

   #158089

   Purpose

   Expresses ACE2 in mammalian cells

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   ACE2 (ACE2 Human)
   

   Expression

   Mammalian

   Mutation

   See depositor comments below

   Available Since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1-IL2sp-6His-Asprosin plasmid

   Plasmid

   #190683

   Purpose

   Expresses His-tagged asprosin in mammalian cells. An IL2 signal peptide at N-terminus allows secretion of asprosin from the transduced cells.

   Depositor

   Atul Chopra

   Article

   Mishra et al STAR Protoc. 2022 Dec 16;3(4):101762. do

   Insert

   IL2-6xHis-Asprosin
   

   Tags

   IL2 signal peptide, 6xHis

   Expression

   Mammalian

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. T7 promoter + filler DNA reporter plasmid

   Plasmid

   #156454

   Purpose

   A mutational reporter plasmid that can gain Kanamycin and/or Tetracycline resistance upon mutation. Contains T7 promoter.

   Depositor

   Matthew D. Shoulders

   Article

   Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

   Inserts

   NeoR/KanR
   TcR
   

   Expression

   Bacterial

   Mutation

   ATG start codon mutated to ACG

   Promoter

   Em7 and tet

   Available Since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Dual opposing T7 promoters reporter plasmid

    Plasmid

    #156457

    Purpose

    A mutational reporter plasmid that can gain Kanamycin resistance upon mutation. Contains two T7 promoters flanking the kanamycin resistance gene.

    Depositor

    Matthew D. Shoulders

    Article

    Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

    Insert

    NeoR/KanR
    

    Expression

    Bacterial

    Mutation

    ATG start codon mutated to ACG

    Promoter

    Em7

    Available Since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCDH-CMV-RPS4X-3xHA plasmid

    Plasmid

    #192246

    Purpose

    overexpression vector of RPS4X-3XHA

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    RPS4X CDS without stop coden (RPS4X Human)
    

    Use

    Lentiviral

    Tags

    3xHA

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PGK+ plasmid with onboard mCherry cassette

    Plasmid

    #169745

    Purpose

    PGK+ plasmid that also encodes separate mCherry cassette. mCherry is used to monitor plasmid dosage.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    eGFP
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    PGK-tetO2

    Available Since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Equalizer-H plasmid with onboard mCherry cassette

    Plasmid

    #169733

    Purpose

    Equalizer-H plasmid that encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    tetR-P2A-eGFP-miR(FF3) target-miR(FF3)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV-tetO2

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CMV+ plasmid with onboard mCherry cassette

    Plasmid

    #169741

    Purpose

    CMV+ plasmid that also encodes a separate mCherry cassette. mCherry is used to monitor plasmid dosage.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    eGFP
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    pCMV-tetO2

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLK88 - Edit-directing plasmid base

    Plasmid

    #98814

    Purpose

    Edit-directing plasmid base

    Depositor

    Leonid Kruglyak

    Insert

    CAN1.y gRNA
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    July 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Gr4-T2A-QF2 HDR plasmid

    Plasmid

    #140944

    Purpose

    Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti Gr4 gene

    Depositor

    Leslie Vosshall

    Article

    Jove et al Neuron. 2020 Dec 23;108(6):1163-1180.e12

    Insert

    Gr4-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Gr4-right-HDR-arm
    

    Available Since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. RPS4X sgRNA-3 lentiCRISPR v2-Blast plasmid

    Plasmid

    #192233

    Purpose

    lentiviral vector expressing sgRNA-3 targeting eIF3 binding site of RPS4X 5'UTR

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    sgRNA-3 targeting eIF3 binding site of RPS4X 5'UTR (RPS4X Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. RPS15A sgRNA-5 lentiCRISPR v2-Blast plasmid

    Plasmid

    #192230

    Purpose

    lentiviral vector expressing sgRNA-5 targeting eIF3 binding site of RPS15A 5'UTR

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    sgRNA-5 targeting eIF3 binding site of RPS15A 5'UTR (RPS15A Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. RPS4X sgRNA-4 lentiCRISPR v2-Blast plasmid

    Plasmid

    #192234

    Purpose

    lentiviral vector expressing sgRNA-4 targeting eIF3 binding site of RPS4X 5'UTR

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    sgRNA-4 targeting eIF3 binding site of RPS4X 5'UTR (RPS4X Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. RPS15A sgRNA-4 lentiCRISPR v2-Blast plasmid

    Plasmid

    #192229

    Purpose

    lentiviral vector expressing sgRNA-4 targeting eIF3 binding site of RPS15A 5'UTR

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    sgRNA-4 targeting eIF3 binding site of RPS15A 5'UTR (RPS15A Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge