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Showing: 101 - 120 of 1687 results

  3. lenti-dCas9-ZIM3-KRAB-BFP

    Plasmid
    #196712
    Purpose
    CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3
    Depositor
    Bernhard Schmierer
    Insert
    dCas9-KRAB(ZIM3)-T2A-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Mutation
    D10A, N863A
    Promoter
    EF1a
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196712-plasmid-map-sequence-id-388051

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  5. pCSD_2xNLS-SpCas9MT3-NLS-FKBP_SL

    Plasmid
    #107305
    Purpose
    Expresses R1335K mutant SpCas9 fused to FKBP with extra NLS in mammalian cells
    Depositor
    Scot Wolfe
    Insert
    SpCas9
    Use
    CRISPR
    Tags
    2x NLS and FKBP
    Expression
    Mammalian
    Mutation
    R1335K and fused to FKBP
    Promoter
    CMV IE94
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107305-plasmid-map-sequence-id-204646

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  6. pAAV-MP-84-spCas9-U6-gRNA-tlock

    Plasmid
    #206936
    Purpose
    Expression of spCas9 endonuclease and gRNA in an AAV vector allowing packaging of viral genome smaller than 5 kb.
    Depositor
    Markus Grompe
    Insert
    spCas9
    Use
    AAV
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    MP-84
    Available since
    Sept. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    206936-plasmid-map-sequence-id-407723

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  10. pCW-Tet-Cas9-BFP-PGK-Blast

    Plasmid
    #196720
    Purpose
    Tet-inducible expression of Cas9-T2A-TagBFP
    Depositor
    Bernhard Schmierer
    Insert
    Cas9-T2A-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    3xFLAG-SV40NLS and Nucleoplasmin NLS
    Expression
    Mutation
    Promoter
    TRE, PGK
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196720-plasmid-map-sequence-id-389756

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  11. pCW-Tet-Cas9-miRFP670nano-PGK-Blast

    Plasmid
    #196722
    Purpose
    Tet-inducible expression of Cas9-T2A-miRFP670nano
    Depositor
    Bernhard Schmierer
    Insert
    Cas9-T2A-miRFP670nano
    Use
    CRISPR and Lentiviral
    Tags
    3xFLAG-SV40NLS and Nucleoplasmin NLS
    Expression
    Mutation
    Promoter
    TRE, PGK
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196722-plasmid-map-sequence-id-389804

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  12. pISV-CRISPR/Cas9PGmUbi

    Plasmid
    #209189
    Purpose
    Expression of Cas9 in the model legume Medicago truncatula, Gmubi promoter (PGmubi), DsRed and Basta selection
    Depositor
    Christian Staehelin
    Inserts
    Cas9
    DsRed(deltaB)
    Use
    Tags
    NLS
    Expression
    Plant
    Mutation
    missing BsaI site and plant-codon optimized with …
    Promoter
    CaMV 35S and GmUbi from Glycine max (L.) Merr. cv…
    Available since
    Jan. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    209189-plasmid-map-sequence-id-419145

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  17. bbCas9pluspAAA

    Plasmid
    #82581
    Purpose
    The plasmid encodes the T7 promoter, Cas9 (from pX330 #42230) and a stretch of poly-A. After linearization with restriction enzyme SapI It is used to produce in vitro transcribed mRNA
    Depositor
    Ivo Huijbers
    Article
    Pritchard et al Methods Mol Biol. 2017;1642:21-35. doi:
    Insert
    Cas9
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    82581-plasmid-map-sequence-id-228465

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  19. lenti-Cas9-Exo-Blast-BFP

    Plasmid
    #196721
    Purpose
    Sortable and selectable SpCas9 fused to exodeoxyribonuclease I (sbcB) from E. coli. For the creation of longer deletions.
    Depositor
    Bernhard Schmierer
    Insert
    Cas9-Exo1-T2A-BSD-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196721-plasmid-map-sequence-id-389753

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Showing: 101 - 120 of 1687 results