We narrowed to 4,622 results for: TIL;

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pFA6a-kanMX6

Plasmid

#39296

Depositor

Jurg Bahler, John Pringle

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

KanR

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Available Since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only

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Endo-APEX2

Plasmid

#215553

Purpose

Stable, inducible expression in endosome

Depositor

Nevan Krogan

Article

Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

Insert

EGFP-2xFYVE-APEX2-NES

Use

Lentiviral

Available Since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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Sp_PE

Plasmid

#212038

Purpose

Bacterial expression of the PE2 proteins for genome editing in E. coli

Depositor

Quanjiang Ji

Article

Zhang et al Nat Commun. 2024 Jan 27;15(1):825. doi:

Insert

PE2

Use

CRISPR

Promoter

L-arabinose-inducible promoter PBAD

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT/TO Plk4-mCherry

Plasmid

#80269

Purpose

mammalian expression plasmid for Plk4-mCherry

Depositor

Andrew Holland

Article

Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

Insert

Plk4 (PLK4 Human)

Tags

mCherry

Expression

Mammalian

Available Since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pQFT-mNGsacB

Plasmid

#203676

Purpose

pQFT with mNeonGreen and sacB under control of PQJ

Depositor

Urs Jenal

Article

Klotz et al Appl Environ Microbiol. 2023 May 18:e002

Inserts

mNeonGreen
sacB

Expression

Bacterial

Mutation

Has synonymous (silent) mutations to remove BsrGI…

Promoter

PQJ

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pF hPGK mc2 IRES blast

Plasmid

#206239

Purpose

For expressing cloned exons as circular RNA.

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pF hPGK mc2 IRES hygro

Plasmid

#206238

Purpose

For expressing cloned exons as circular RNA.

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pf CAG mc2 IRES blast

Plasmid

#206235

Purpose

For expressing cloned exons as circular RNA.

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pf CAG mc2 IRES puro

Plasmid

#206233

Purpose

For expressing cloned exons as circular RNA.

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pF CAG mc2 IRES hygro

Plasmid

#206234

Purpose

For expressing cloned exons as circular RNA.

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pUC57-CmYLCV

Plasmid

#205245

Purpose

For plant prime editing in wheat plants or monocotyledons protoplasts

Depositor

Zong Yuan

Article

Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

Insert

CmYLCV-csy4_binding_site-esgRNA-tevopreQ1-csy4_binding_site

Use

CRISPR

Expression

Plant

Promoter

CmYLCV

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pHRm-GFP(1-10)-VP64-NLS

Plasmid

#70228

Purpose

Expresses GFP(1-10)-VP64-NLS in mammalian cells

Depositor

Bo Huang

Article

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

GFP(1-10)-VP64-NLS

Use

Lentiviral

Expression

Mammalian

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pLVCT-tTR-KRAB

Plasmid

#11643

Purpose

Tet-regulated (Tet-on) lentiviral vector for transgene (CAG promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

Depositor

Patrick Aebischer, Didier Trono

Article

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

CAG promoters, GFP, tTR-KRAB, Tet-on

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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pFREE_Zeo

Plasmid

#92053

Purpose

Allows inducible expression of gRNAs and Cas9 for plasmid curing.

Depositor

Morten Norholm

Article

Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

Inserts

Cas9
gRNA array

Use

CRISPR

Promoter

pRhamBAD and ptet

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pT3-EF1α-Dest

Plasmid

#133299

Purpose

The pT3-EF1α destination vector without any insert which can be used for LR reaction.

Depositor

Xin Chen

Article

Lee et al Hepatology. 2008 Apr;47(4):1200-10. doi:

Type

Empty backbone

Expression

Mammalian

Promoter

EF1α

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pFREE_Amp

Plasmid

#92051

Purpose

Allows inducible expression of gRNAs and Cas9 for plasmid curing.

Depositor

Morten Norholm

Article

Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

Inserts

Cas9
gRNA array

Use

CRISPR

Promoter

pRhamBAD and ptet

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pBR322-Pdp1_NTD-Cas9

Plasmid

#198275

Purpose

For loading SpCas9 into PVCs; also contains PVC regulatory genes

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Pdp1_NTD-Cas9

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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CROPseq-multi-v2-SpCas9-P2A-Puro-BsmBI_entry (pRW1512)

Plasmid

#225756

Purpose

Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with expression of SpCas9 and Puromycin resistance; v2 is compatible with T7 in vitro transcription detection

Depositor

Paul Blainey

Article

Walton et al bioRxiv 2024.03.17.585235

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pHCMV-SS-antiHAscFv-TM

Plasmid

#198302

Purpose

Displays anti-HA scFv from the surface of mammalian cells

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

pHCMV-SS-antiHAscFv-TM

Expression

Mammalian

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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YSBE 731 : pHYG_4xFLAG

Plasmid

#92090

Purpose

Plasmid for tagging proteins in Cryptococcus neoformans; 4xFLAG tag, HYG selection

Depositor

Yong-Sun Bahn

Article

So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

Insert

4xFlag

Use

tagging proteins in cryptococcus neoforman

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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