We narrowed to 6,901 results for: plasmid dna

1. pcDNA3_SARS-CoV-Spike[BtKY72 RBD]

   Plasmid

   #190081

   Purpose

   express chimeric SARS-CoV spike with the BtKY72 receptor binding domain

   Depositor

   Kenneth Matreyek

   Article

   Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

   Insert

   SARS-CoV S (S Synthetic)
   

   Use

   Tags

   Rhodopsin C9

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3_SARS-CoV-Spike[PRD0038 RBD]

   Plasmid

   #190085

   Purpose

   express chimeric SARS-CoV spike with the PRD0038 receptor binding domain

   Depositor

   Kenneth Matreyek

   Article

   Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

   Insert

   SARS-CoV S (S Synthetic)
   

   Use

   Tags

   Rhodopsin C9

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5-hygro-eGFP-STOP-HEC1

   Plasmid

   #114026

   Purpose

   Inducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines. Due to insertion of a STOP-codon after the eGFP-tag, this plasmid only expressed eGFP.

   Depositor

   Geert Kops

   Article

   Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

   Insert

   eGFP-STOP-HEC1 (NDC80 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Start codon of Hec1 is mutated to stop codon

   Promoter

   Available Since

   Sept. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+)-HLA-Flag-natT1R3

   Plasmid

   #113950

   Purpose

   mammalian expression plasmid for FLAG-tagged human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

   Depositor

   Erik Procko

   Article

   Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

   Insert

   T1R3 (TAS1R3 Human)
   

   Use

   Tags

   FLAG and Signal/leader sequence from HLA class I …

   Expression

   Mammalian

   Mutation

   residues 21-852

   Promoter

   Available Since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5-EGFP-AID-BubR1

   Plasmid

   #47330

   Purpose

   for plasmid integration using the Flp-In system. AID at N-terminal

   Depositor

   Don Cleveland

   Article

   Holland et al Proc Natl Acad Sci U S A. 2012 Dec 4;109

   Insert

   BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B Human)
   

   Use

   Tags

   AID and GFP

   Expression

   Mammalian

   Mutation

   siRNA resistant

   Promoter

   Available Since

   Sept. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-HCMVgO-sfGFP (TB40/E)

   Plasmid

   #136451

   Purpose

   Mammalian expression plasmid for HCMV glycoprotein O (TB40/E strain) fused to superfolder GFP

   Depositor

   Erik Procko

   Article

   Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

   Insert

   glycoprotein O (UL74 Human betaherpesvirus 5)
   

   Use

   Tags

   Gly/Ser-rich linker (GGSGGSGSGG) (includes BamHI …

   Expression

   Mammalian

   Mutation

   Codon-optimized for human cell expression.

   Promoter

   CMV

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5-FRT/TO-mNeonGreen-BRD4

   Plasmid

   #204692

   Purpose

   This plasmid allows for inducible expression of short BRD4 isoform tagged with mNeonGreen, in mammalian cells.

   Depositor

   Michael Rosen

   Article

   Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

   Insert

   BRD4 (BRD4 Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   CMV

   Available Since

   Aug. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-HCMVgO-sfGFP (Merlin)

   Plasmid

   #136452

   Purpose

   Mammalian expression plasmid for HCMV glycoprotein O (Merlin strain) fused to superfolder GFP

   Depositor

   Erik Procko

   Article

   Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

   Insert

   glycoprotein O (UL74 Human betaherpesvirus 5)
   

   Use

   Tags

   Gly/Ser-rich linker (GGSGGSGSGG) (includes BamHI …

   Expression

   Mammalian

   Mutation

   Codon-optimized for human cell expression.

   Promoter

   CMV

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-HCMVgH-8his (TB40/E)

   Plasmid

   #136445

   Purpose

   Mammalian expression plasmid for HCMV glycoprotein H (TB40/E strain) extracellular domain with 8his purification tag

   Depositor

   Erik Procko

   Article

   Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

   Insert

   glycoprotein H (UL75 Human betaherpesvirus 5)
   

   Use

   Tags

   Short GGSG linker and 8his tag

   Expression

   Mammalian

   Mutation

   Extracellular domain (a.a. 1-717). Codon-optimize…

   Promoter

   CMV

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-HCMVgO-8his (TB40/E)

    Plasmid

    #136453

    Purpose

    Mammalian expression plasmid for HCMV glycoprotein O (TB40/E strain) with an 8his tag

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    envelope glycoprotein O (UL74 Human betaherpesvirus 5)
    

    Use

    Tags

    Linker and 8his tag (GGSGHHHHHHHH, includes BamHI…

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.2 mir-1-1 reporter mir16-1 wt

    Plasmid

    #46683

    Purpose

    Expresses wild-type miR-16-1 in mammalian cells

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    mir16-1 wt (MIR16-1 Human, Synthetic)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.2 mir-1-1 reporter mir129-2-wt

    Plasmid

    #46730

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    mir129-2-wt (MIR129-2 Human, Synthetic)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. RGS-6xHis-DRE-1-pcDNA3.1-

    Plasmid

    #52517

    Purpose

    expresses C. elegans DRE-1 in mammalian cells with RGS-6xHis tag at N-terminus

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Insert

    Dre-1 (dre-1 Nematode)
    

    Use

    Tags

    RGS-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(+)Gα13-125-NLuc

    Plasmid

    #207155

    Purpose

    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA13 tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2

    Depositor

    Tom Van Loy, Dominique Schols

    Article

    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

    Insert

    GNA13 (GNA13 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NLuc inserted at position F(125)D

    Promoter

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1(+)Gα12-133-NLuc

    Plasmid

    #207156

    Purpose

    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA12 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

    Depositor

    Tom Van Loy, Dominique Schols

    Article

    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

    Insert

    GNA12 (GNA12 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NLuc inserted at position A(133)F

    Promoter

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. Pmyo-3::hpo-30 gDNA (pBAB204)

    Plasmid

    #117385

    Purpose

    Allows for body-wall muscle expression of HPO-30

    Depositor

    Kavita Babu

    Article

    Sharma et al J Neurosci. 2018 Aug 8;38(32):7072-7087.

    Insert

    genomic DNA of hpo-30 (833 bp)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.2 mir-1-1 reporter mir129-2-2

    Plasmid

    #46728

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    mir129-2-2 (MIR129-2 Human, Synthetic)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    see sequence and publication

    Promoter

    CMV

    Available Since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1(+)-myc-OGA(GS-544)C181-D174N

    Plasmid

    #193999

    Purpose

    plasmid encoding myc-tagged OGA(GS-544)C181-D174N

    Depositor

    Christina Woo

    Article

    Ge et al ACS Chem Biol. 2023 Jan 4. doi: 10.1021/

    Insert

    myc-OGA(GS-544)C181-D174N
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDNA3.3_MERS_D12

    Plasmid

    #170448

    Purpose

    Express MERS WT spike protein with an 12aa deletion on the C-terminal tail. Used for MERS pseudotyped virus production.

    Depositor

    David Nemazee

    Article

    Rogers et al Science. 2020 Aug 21;369(6506):956-963.

    Insert

    MERS spike D12 (S MERS)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    last 12aa deletion in c-terminal tail

    Promoter

    CMV

    Available Since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1(+) Laccase2 MCS Exon Vector

    Plasmid

    #69893

    Purpose

    Expression plasmid for expressing circular RNAs of a desired sequence in mammalian cells

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    Laccase2 (stw Fly)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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