We narrowed to 6,452 results for: plasmid dna

1. pcDNA3.2 mir-1-1 reporter mir16-1 wt

   Plasmid

   #46683

   Purpose

   Expresses wild-type miR-16-1 in mammalian cells

   Depositor

   David Bartel

   Article

   Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

   Insert

   mir16-1 wt (MIR16-1 Human, Synthetic)
   

   Use

   RNAi

   Tags

   V5 and hsa-mir-1-1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.2 mir-1-1 reporter mir129-2-wt

   Plasmid

   #46730

   Depositor

   David Bartel

   Article

   Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

   Insert

   mir129-2-wt (MIR129-2 Human, Synthetic)
   

   Use

   RNAi

   Tags

   V5 and hsa-mir-1-1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. RGS-6xHis-DRE-1-pcDNA3.1-

   Plasmid

   #52517

   Purpose

   expresses C. elegans DRE-1 in mammalian cells with RGS-6xHis tag at N-terminus

   Depositor

   Adam Antebi

   Article

   Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

   Insert

   Dre-1 (dre-1 Nematode)
   

   Use

   Tags

   RGS-6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+)Gα13-125-NLuc

   Plasmid

   #207155

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA13 tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2

   Depositor

   Tom Van Loy, Dominique Schols

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNA13 (GNA13 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NLuc inserted at position F(125)D

   Promoter

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1(+)Gα12-133-NLuc

   Plasmid

   #207156

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA12 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

   Depositor

   Tom Van Loy, Dominique Schols

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNA12 (GNA12 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NLuc inserted at position A(133)F

   Promoter

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Pmyo-3::hpo-30 gDNA (pBAB204)

   Plasmid

   #117385

   Purpose

   Allows for body-wall muscle expression of HPO-30

   Depositor

   Kavita Babu

   Article

   Sharma et al J Neurosci. 2018 Aug 8;38(32):7072-7087.

   Insert

   genomic DNA of hpo-30 (833 bp)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.2 mir-1-1 reporter mir129-2-2

   Plasmid

   #46728

   Depositor

   David Bartel

   Article

   Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

   Insert

   mir129-2-2 (MIR129-2 Human, Synthetic)
   

   Use

   RNAi

   Tags

   V5 and hsa-mir-1-1

   Expression

   Mammalian

   Mutation

   see sequence and publication

   Promoter

   CMV

   Available since

   June 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1(+)-myc-OGA(GS-544)C181-D174N

   Plasmid

   #193999

   Purpose

   plasmid encoding myc-tagged OGA(GS-544)C181-D174N

   Depositor

   Christina Woo

   Article

   Ge et al ACS Chem Biol. 2023 Jan 4. doi: 10.1021/

   Insert

   myc-OGA(GS-544)C181-D174N
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3.3_MERS_D12

   Plasmid

   #170448

   Purpose

   Express MERS WT spike protein with an 12aa deletion on the C-terminal tail. Used for MERS pseudotyped virus production.

   Depositor

   David Nemazee

   Article

   Rogers et al Science. 2020 Aug 21;369(6506):956-963.

   Insert

   MERS spike D12 (S MERS)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   last 12aa deletion in c-terminal tail

   Promoter

   CMV

   Available since

   July 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1(+) Laccase2 MCS Exon Vector

    Plasmid

    #69893

    Purpose

    Expression plasmid for expressing circular RNAs of a desired sequence in mammalian cells

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    Laccase2 (stw Fly)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-Lyn-dInPAkt(R23/25A)

    Plasmid

    #181835

    Purpose

    Phospholipid binding-deficient Lyn-dInPAkt negative-control plasmid.

    Depositor

    Jin Zhang

    Article

    Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

    Insert

    Lyn-dInPAkt
    

    Use

    Tags

    N-terminal targeting sequence from Lyn kinase, dd…

    Expression

    Mammalian

    Mutation

    Arginines 23 and 25 in PH domain mutated to alani…

    Promoter

    CMV

    Available since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1 SP-His-mCherry-HRP-VhHGFP

    Plasmid

    #112157

    Purpose

    Expression and secretion of anti-GFP FLIPPER-bodies, small protein probes for correlated microscopy. FLIPPER-bodies are the fusion of a nanobody with a fluorescent protein and a peroxidase.

    Depositor

    Ben Giepmans

    Article

    de Beer et al Histochem Cell Biol. 2018 Mar;149(3):261

    Insert

    anti-GFP FLIPPER-body
    

    Use

    Tags

    His and Thrombin cleavage site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-CAG-sbGLuc-hGtARC2-EYFP

    Plasmid

    #114111

    Purpose

    fusion protein of Gaussia luciferase variant slow burn, Guillardia theta anion channelrhodopsin-2, and enhanced yellow fluorescent protein for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Prakash et al J Neurosci Res. 2018 Aug 28. doi: 10.100

    Insert

    Gaussia luciferase, slow burn; Guillardia theta anion channelrhodopsin-2, human codon optimized
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.2 mir-1-1 reporter dme-mir-2a-1

    Plasmid

    #46664

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    dme-mir-2a-1
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1 Cas9-P2A-dTomato-PS9

    Plasmid

    #231911

    Purpose

    For packaging spCas9, dTomato mRNA into SARS-CoV-2 virus-like particles (VLPs) with a 'PS9' packaging signal

    Depositor

    Sriram Neelamegham

    Article

    Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Insert

    Cas9-P2A-dTomato
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1 Cas9-P2A-dTomato-T20

    Plasmid

    #231912

    Purpose

    For packaging spCas9, dTomato mRNA into SARS-CoV-2 virus-like particles (VLPs) with a 'T20' packaging signal

    Depositor

    Sriram Neelamegham

    Article

    Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Insert

    Cas9-P2A-dTomato
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.2 mir-1-1 reporter dme-let-7

    Plasmid

    #46662

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    dme-let-7
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.2 mir-1-1 reporter dme-mir-34

    Plasmid

    #46665

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    dme-mir-34
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA5/FRT/TO-GAlphasS-RLuc8

    Plasmid

    #140980

    Purpose

    Encodes a G alpha subunit (GNAS2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlphasS-RLuc8
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3 Flag C/EBPb LAP

    Plasmid

    #66979

    Purpose

    Expression plasmid for mouse C/EBPb LAP; adds Flag tag to the N-terminus of protein

    Depositor

    Christopher Vakoc

    Article

    Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

    Insert

    C/EBP beta (Cebpb Mouse)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 15, 2015

    Availability

    Academic Institutions and Nonprofits only