We narrowed to 7,392 results for: bre

1. GFP-Rab27B T23N

   Plasmid

   #89450

   Purpose

   Expression of GFP-tagged human Rab27B T23N in mammalian cells

   Depositor

   Wendy Westbroek

   Article

   Hendrix et al J Natl Cancer Inst. 2010 Jun 16;102(12):

   Insert

   human Rab27B T23N (RAB27B Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   T23N

   Promoter

   CMV

   Available since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLenti-xCas9RA-P2A-Puro

   Plasmid

   #110871

   Purpose

   Lentiviral vector for constitutive expression of xCas9(3.7)RA in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   xCas9(3.7)RA
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   A262T, R324L, S409I, E480K, E543D, M694I, E1219V

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Anti-VGlut1 [N28/9R]

   Plasmid

   #128638

   Purpose

   Mammalian Expression Plasmid of anti-VGlut1 (Rat). Derived from hybridoma N28/9.

   Depositor

   James Trimmer

   Article

   Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

   Insert

   anti-VGlut1 (Rat) recombinant mouse monoclonal antibody (Slc17a7 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual CMV

   Available since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFastBac1_GI.1_Q62C-A140C

   Plasmid

   #162581

   Purpose

   Expresses norovirus GI.1 VP1 protein with mutations Q62C-A140C in Sf9 insect cells

   Depositor

   Peter Kwong

   Article

   Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

   Insert

   VP1 protein from human norovirus GI.1
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Glutamine 62 changed to Cysteine and Alanine 140 …

   Promoter

   polyhedring

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. dCas9-NLS-gs10-cERb447

   Plasmid

   #188257

   Purpose

   Plasmid ensures constitutive expression of the C-terminal fragment of split ERbeta (aa 447-501), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus

   Depositor

   Roman Jerala

   Article

   Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

   Insert

   cERb447 (ESR1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBabepuro3-p18 A61D

   Plasmid

   #24937

   Depositor

   Lynda Chin

   Article

   Wiedemeyer et al Cancer Cell. 2008 Apr . 13(4):355-64.

   Insert

   p18 (CDKN2C Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   A61D

   Promoter

   Available since

   June 10, 2010

   Availability

   Academic Institutions and Nonprofits only

7. P-rTetO-mCherry-T2A-RPL22-1xV5

   Plasmid

   #170325

   Purpose

   Tetracycline-inducible expression of V5 and mCherry tagged RPL22

   Depositor

   Hemali Phatnani

   Article

   Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

   Insert

   RPL22 (RPL22 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBabe DN-FOXO1-HA neo

   Plasmid

   #45814

   Depositor

   Kevin Janes

   Article

   Wang et al Proc Natl Acad Sci U S A. 2011 Oct 4;108

   Insert

   FOXO1 (FOXO1 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   aa1-255

   Promoter

   Available since

   June 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Lenti-DIO-P-Ef1a-mCherry-T2A-RPL22-1xV5

   Plasmid

   #170328

   Purpose

   Double-floxed inverse Orientation V5 and mCherry tagged RPL22

   Depositor

   Hemali Phatnani

   Article

   Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

   Insert

   RPL22 (RPL22 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. px330 Rosa26 Gu1 (LH416)

    Plasmid

    #99629

    Purpose

    Plasmid used to target the wildtype Rosa26 allele with Cas9

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSHUT4-eYFP

    Plasmid

    #133094

    Purpose

    Agrobacterium T-DNA overexpression cassette comprising constitutive TEF-1alpha fused to reporter eYFP. Integrates near beta-tubulin gene in F. solani genome. Replace eYFP w/ XhoI BamHI

    Depositor

    Jens Laurids Sørensen

    Article

    Nielsen et al Fungal Biol Biotechnol. 2019 Dec 11;6:25

    Insert

    enhanced yellow flourescent protein
    

    Use

    Fungal expression

    Tags

    Expression

    Mutation

    Promoter

    TEF-1α

    Available since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. GFP-Rab27B GG

    Plasmid

    #89451

    Purpose

    Expression of GFP-tagged human Rab27B GG in mammalian cells

    Depositor

    Wendy Westbroek

    Article

    Hendrix et al J Natl Cancer Inst. 2010 Jun 16;102(12):

    Insert

    human Rab27B GG (RAB27B Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    GG (geranyl geranyl site is mutated)

    Promoter

    CMV

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBabe JunD-HA neo

    Plasmid

    #58486

    Purpose

    mammalian expression of mouse JunD

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

    Insert

    JunD (Jund Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAG-Dcst1-3xFLAG

    Plasmid

    #183542

    Purpose

    Expression vector of mouse DC-STAMP domain containing 1 (Dcst1) tagged with 3xFLAG at C-terminus.

    Depositor

    Masahito Ikawa

    Article

    Noda et al Commun Biol. 2022 Apr 7;5(1):332. doi: 1

    Insert

    DC-STAMP domain containing 1 (Dcst1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Anti-PICK1 [L20/8R]

    Plasmid

    #128630

    Purpose

    Mammalian Expression Plasmid of anti-PICK1 (Human). Derived from hybridoma L20/8.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-PICK1 (Human) recombinant mouse monoclonal antibody (PICK1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Oct. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti-BE4RA-P2A-Puro

    Plasmid

    #112672

    Purpose

    Lentivirus for constitutive expression of BE4RA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE4RA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    NLS sequence at the N-terminus and D10A

    Promoter

    EF1s

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEN_TTmiRc2_3xflag_NRF2

    Plasmid

    #136527

    Purpose

    Used as a donor vector containing N-term 3xFLAG to clone into pSLIK

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    NRF2 (NFE2L2 Human)
    

    Use

    Entry vector for gateway cloning

    Tags

    3x FLAG

    Expression

    Mutation

    Promoter

    Available since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEF1α-mouse-full-length-CHMP3-HA

    Plasmid

    #154176

    Purpose

    expresses mouse CHMP3 in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

    Insert

    CHMP3 (Chmp3 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Anti-Neuroligin-1 [N97A/31R]

    Plasmid

    #114554

    Purpose

    Mammalian Expression Plasmid of anti-Neuroligin-1 (Rat). Derived from hybridoma N97A/31.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-Neuroligin-1 (Rattus norvegicus) recombinant mouse monoclonal antibody (Nlgn1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSLIK_hygro_BirA_CDKN1A

    Plasmid

    #136538

    Purpose

    Lentiviral expression vector for an inducible BirA-CDKN1A

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    BirA-CDKN1A (CDKN1A )
    

    Use

    Lentiviral; Destinatioin vector for gateway cloni…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLX302 ID2-V5 puro

    Plasmid

    #83096

    Purpose

    Lentiviral vector for constitutive expression of human ID2 with C-terminal V5 tag

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    ID2 (ID2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

22. TET-pLKO.1 PURO shID2 #2

    Plasmid

    #83087

    Purpose

    Lentiviral shRNA vector for inducible knockdown of human ID2

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    shID2 (ID2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

23. LoxP2-Mb2Tomato-2A (SO240)

    Plasmid

    #99614

    Purpose

    To clone gene of interest downstream of LoxP2-Mb2Tomato-2A cassette

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    Mb2Tomato
    

    Use

    Tags

    T2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTRF.1 udsVenus (P_JUND)

    Plasmid

    #58692

    Purpose

    A rapidly responsive reporter of endogenous JUND promoter activity (Lentiviral expression vector for reporter ultradestabilized Venus)

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

    Insert

    endogenous promoter of JUND (JUND Human)
    

    Use

    Lentiviral

    Tags

    udsVenus

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

25. pLenti6.3/TO/V5-DEST-IGFBP2-Clover

    Plasmid

    #191008

    Purpose

    Lentiviral expression vector for IGFBP2 with a fluorescent Clover tag

    Depositor

    Johanna Ivaska

    Article

    Conway et al Sci Adv. 2023 Jul 14;9(28):eadg1840. doi

    Insert

    Insulin-Like Growth Factor Binding Protein 2 (IGFBP2 Human)
    

    Use

    Lentiviral

    Tags

    Clover

    Expression

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. LoxP1-Mb2YFP-2A (SO244)

    Plasmid

    #99613

    Purpose

    To clone gene of interest downstream of LoxP1-Mb2YFP-2A cassette

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    Mb2EYFP
    

    Use

    Tags

    T2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. LoxP2-H2B-EGFP-V5-2A (SO107)

    Plasmid

    #99617

    Purpose

    To clone gene of interest downstream of LoxP2-H2B-EGFP-V5-2A

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    H2B-EGFP-V5
    

    Use

    Tags

    T2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLenti-BE3RA-P2A-GFP-PGK-Puro

    Plasmid

    #110868

    Purpose

    Lentiviral vector for constitutive expression of BE3RA-P2A-GFP in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3RA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D10A

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBabe RFP-DN-FOXO1-HA neo

    Plasmid

    #45813

    Depositor

    Kevin Janes

    Article

    Wang et al Proc Natl Acad Sci U S A. 2011 Oct 4;108

    Insert

    FOXO1 (FOXO1 Human)
    

    Use

    Retroviral

    Tags

    HA and RFP

    Expression

    Mammalian

    Mutation

    aa1-255

    Promoter

    Available since

    June 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSLIK TT 3xFLAG ID2 hygro

    Plasmid

    #98394

    Purpose

    Lentiviral vector for inducible expression of human ID2 with 3xFLAG N-terminal tag

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    ID2 (ID2 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. LoxP3-HA-H2B-Cerulean-2A (SO104)

    Plasmid

    #99618

    Purpose

    To clone gene of interest downstream of LoxP3-HA-H2B-Cerulean-2A

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    HA-H2B-Cerulean
    

    Use

    Tags

    T2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLKO.1-shGBP1.1.mKO2

    Plasmid

    #85209

    Purpose

    TRCN0000116119 (Target CGACGAAAGGCATGTACCATA) for silencing GBP1 gene and express monomeric Kusabira-Orange2.

    Depositor

    Sandra Martha Gomes Dias

    Article

    Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

    Insert

    GBP1 (guanylate binding protein 1) (GBP1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RNA polymerase III promoter for human U6 snRNA fo…

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSLIK JunD-HA neo

    Plasmid

    #58515

    Purpose

    Lentiviral expression vector for an inducible HA-tagged mouse JunD

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

    Insert

    JunD (Jund Mouse)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

34. sgRNA BRCA1 C64Y

    Plasmid

    #139326

    Purpose

    Plasmid expressing a sgRNA to introduce BRCA1 C64Y using base editing

    Depositor

    Alberto Ciccia

    Article

    Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

    Insert

    sgRNA to insert BRCA1 C64Y using base editing
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAAV-RSV-GFP-U6-Rosa26 gRNA (SpyCas9 scaffold)

    Plasmid

    #120296

    Purpose

    AAV vector; encodes GFP as well as a U6-driven Rosa26-targeting gRNA (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

    Insert

    Rosa26 gRNA (SpCas9 scaffold)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Anti-Pan-Shank [N23B/49R]

    Plasmid

    #128637

    Purpose

    Mammalian Expression Plasmid of anti-Pan-Shank. Derived from hybridoma N23B/49.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-Pan-Shank Pan recombinant mouse monoclonal antibody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Control-cx43.4-48H-pPRISM

    Plasmid

    #117801

    Purpose

    Control to test technique on a target that is known to work

    Depositor

    Jeffrey Essner

    Article

    Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

    Insert

    TagRFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLenti-HF1RA-PGK-Puro

    Plasmid

    #110860

    Purpose

    Lentiviral vector for constitutive expression of Cas9-HF1RA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-HF1RA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    R661A, Q695A, Q926A, and NLS sequence at the N-te…

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pBabepuro3-p18 F37I

    Plasmid

    #24936

    Depositor

    Lynda Chin

    Article

    Wiedemeyer et al Cancer Cell. 2008 Apr . 13(4):355-64.

    Insert

    p18 (CDKN2C Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    F37I

    Promoter

    Available since

    June 23, 2010

    Availability

    Academic Institutions and Nonprofits only

40. LoxP3-Mb2-HA-mTFP1-2A (SO157)

    Plasmid

    #99615

    Purpose

    To clone gene of interest downstream of LoxP3-Mb2-HA-mTFP1-2A

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    Mb2-HA-mTfp1
    

    Use

    Tags

    T2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. Anti-Neuroligin-4* [N98/7R]

    Plasmid

    #140081

    Purpose

    Mammalian Expression Plasmid of anti-Neuroligin-4* (Mouse). Derived from hybridoma N98/7.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-Neuroligin-4* (Mouse) recombinant mouse monoclonal antibody (Nlgn4l Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pTRIP-SFFV-mtagBFP-2A RIGI K270A

    Plasmid

    #167290

    Purpose

    Lentiviral expression construct encoding a TagBFP in frame with a self-cleaving catalytically dead (K270A) RLR sensor RIG-I

    Depositor

    Agnel Sfeir

    Article

    Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

    Insert

    DDX58 (RIGI Human)
    

    Use

    Lentiviral

    Tags

    tagBFP

    Expression

    Mammalian

    Mutation

    K270A

    Promoter

    SFFV

    Available since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. HA-CK2alpha’ V67A/I175A (pJD24)

    Plasmid

    #37363

    Depositor

    David Litchfield

    Article

    Duncan et al Mol Cell Proteomics. 2008 Jun;7(6):1077-

    Insert

    CK2α' V67A/I175A (CSNK2A2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    V67A, I175A

    Promoter

    CMV

    Available since

    July 17, 2012

    Availability

    Academic Institutions and Nonprofits only

44. dCas9-NLS-gs10-cDHFR174

    Plasmid

    #188256

    Purpose

    Plasmid ensures constitutive expression of the C-terminal fragment of split DHFR (aa 1-173), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus

    Depositor

    Roman Jerala

    Article

    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

    Insert

    cDHFR174 (DHFR Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pLenti-HF1RA-P2A-Puro

    Plasmid

    #110853

    Purpose

    Lentiviral vector for constitutive expression of Cas9-HF1RA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-HF1RA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    R661A, Q695A, Q926A, and NLS sequence at the N-te…

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pCW57.1 DOX off blast NFS1

    Plasmid

    #160801

    Purpose

    Express Dox repressible NFS1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    NFS1 (NFS1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pEGFP-N1-CATULIN

    Plasmid

    #118979

    Purpose

    Expresses full length of Catulin-GFP (Mus musculus). Contains CMV and T3 promoter for mammalian expression and mRNA production.

    Depositor

    Tomasz Prószyński

    Article

    Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

    Insert

    Alpha Catulin (Ctnnal1 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. Tet-pLKO-puro shPOLE1_1

    Plasmid

    #160810

    Purpose

    Express Dox repressible shPOLE1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLE1_1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pLPCX2-HA-GATA3 C1(259-443)

    Plasmid

    #89832

    Purpose

    Express GATA3 C1 in mammalian cells

    Depositor

    Shengyu Yang

    Article

    Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

    Insert

    Gata3 (Gata3 Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    C1 (AA 259-443)

    Promoter

    CMV

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. TET-pLKO.1 PURO shID2 #1

    Plasmid

    #83086

    Purpose

    Lentiviral shRNA vector for inducible knockdown of human ID2

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    shID2 (ID2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only