Addgene

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pIT5_KW

Plasmid

#173776

Purpose

Bacterial genomic integration vector

Depositor

Keith Shearwin

Article

Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pIT5_KD

Plasmid

#173771

Purpose

Bacterial genomic integration vector

Depositor

Keith Shearwin

Article

Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pIT5_KL

Plasmid

#173770

Purpose

Bacterial genomic integration vector

Depositor

Keith Shearwin

Article

Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pIT5_KP_LacI

Plasmid

#173780

Purpose

Bacterial genomic integration vector, LacI repressor

Depositor

Keith Shearwin

Article

Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pIT5_KD_NahR

Plasmid

#173778

Purpose

Bacterial genomic integration vector, NahR repressor

Depositor

Keith Shearwin

Article

Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pIT5_KW_CymR

Plasmid

#173777

Purpose

Bacterial genomic integration vector, CymR repressor

Depositor

Keith Shearwin

Article

Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pKT0103

Plasmid

#8718

Depositor

Kurt Thorn

Article

Sheff et al Yeast 2004 Jun;21(8):661-70.

Insert

yEVenus

Use

Tags

Codon optimized linker

Expression

Yeast

Mutation

yEGFP with F46L, S65G, V68L, S72A, M153T, V163A, …

Promoter

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

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pIB3

Plasmid

#25452

Depositor

Benjamin Glick

Article

Sears et al Yeast. 1998 Jun 15;14(8):783-90.

Insert

Pichia pastoris HIS4 (PAS_chr1-4_0160 Pichia pastoria)

Use

Pichia pastoris expression

Tags

Expression

Mutation

Promoter

Available since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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YIplac211-GGA2-HaloTagx2

Plasmid

#140150

Purpose

Plasmid for pop-in/pop-out replacement of Gga2 with Gga2-HaloTagx2

Depositor

Benjamin Glick

Article

Casler et al Elife. 2020 Jun 25;9. pii: 56844. doi: 1

Insert

GGA2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pKT0174

Plasmid

#8720

Depositor

Kurt Thorn

Article

Sheff et al Yeast 2004 Jun;21(8):661-70.

Insert

yECFP

Use

Tags

Codon optimized linker

Expression

Yeast

Mutation

yEGFP with F64L, S65T, Y66W, N144I, M153T, V163A

Promoter

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

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Gatewayp221oxCerulean-oxVenus

Plasmid

#68047

Purpose

oxCerulean-oxVenus in Gateway [1-2] plasmid p221

Depositor

Dana Miller

Article

Chapin et al Aging (Albany NY). 2015 Jun 30.

Insert

p221oxCerulean-oxVenus

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pIB1

Plasmid

#25450

Depositor

Benjamin Glick

Article

Sears et al Yeast. 1998 Jun 15;14(8):783-90.

Insert

Pichia pastoris HIS4 (PAS_chr1-4_0160 Pichia pastoria)

Use

Pichia pastoris expression

Tags

Expression

Mutation

Promoter

Available since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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pJ2048.2_Gs

Plasmid

#140672

Purpose

1-color stripe (“GREEN”)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

1-color stripe (“GREEN”)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pKT0211

Plasmid

#8734

Depositor

Kurt Thorn

Article

Sheff et al Yeast 2004 Jun;21(8):661-70.

Insert

yECitrine A206R

Use

Tags

Codon optimized linker

Expression

Yeast

Mutation

yEGFP with S65G, V68L, Q69M, S72A, T203Y, A206R

Promoter

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

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TJF117

Plasmid

#132572

Purpose

pZE1-oxySp-mCherry

Depositor

Timothy Lu

Article

Muller et al Nat Commun. 2019 Sep 6;10(1):4028. doi:

Insert

oxySp-mCherry-TermT1

Use

Tags

Expression

Bacterial

Mutation

Promoter

oxySp

Available since

Aug. 13, 2022

Availability

Academic Institutions and Nonprofits only

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TJF64

Plasmid

#132573

Purpose

pZE1-katGp-mCherry

Depositor

Timothy Lu

Article

Muller et al Nat Commun. 2019 Sep 6;10(1):4028. doi:

Insert

katGp-mCherry-TermT1

Use

Tags

Expression

Bacterial

Mutation

Promoter

katGp

Available since

Aug. 13, 2022

Availability

Academic Institutions and Nonprofits only

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TJF66

Plasmid

#132574

Purpose

pZE1-ahpCp-mCherry

Depositor

Timothy Lu

Article

Muller et al Nat Commun. 2019 Sep 6;10(1):4028. doi:

Insert

ahpCp-mCherry-TermT1

Use

Tags

Expression

Bacterial

Mutation

Promoter

ahpCp

Available since

Aug. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pJRL2 PHO5pr VYFP (EB#1547)

Plasmid

#14840

Depositor

Erin O'Shea

Article

Raser et al Science. 2004 Jun 18. 304(5678):1811-4.

Insert

PHO5 promoter (PHO5 Budding Yeast)

Use

Tags

VYFP

Expression

Yeast

Mutation

Promoter

Available since

May 25, 2007

Availability

Academic Institutions and Nonprofits only

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pKT0101

Plasmid

#8721

Depositor

Kurt Thorn

Article

Sheff et al Yeast 2004 Jun;21(8):661-70.

Insert

yECFP

Use

Tags

Codon optimized linker

Expression

Yeast

Mutation

yEGFP with F64L, S65T, Y66W, N146I, M153T, V163A

Promoter

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pJ2042.2_invRep

Plasmid

#140688

Purpose

3-color stripe (inverted reporters)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

3-color stripe (inverted reporters)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge