We narrowed to 62,256 results for: cloning
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Plasmid#220958Purposehuman U6-driven expression of ωRNA-v2 scaffold with BsmBI for guide cloingDepositorInsertOgeuIscB-ωRNA-v2 scaffold with BsmBI for guide cloning
UseCRISPRExpressionMammalianAvailable SinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_NE1h
Plasmid#123309PurposeExpresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1h in neuronsDepositorHas ServiceAAV Retrograde and AAV9InsertGPCR activation based NE sensor GRAB_NE1h
UseAAVPromoterhSynAvailable SinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB-gDA3m
Plasmid#208698PurposeExpresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3m in neuronsDepositorHas ServiceAAV1InsertGPCR activation based dopamine (DA) sensor GRAB_gDA3m
UseAAVPromoterhSynAvailable SinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CKIIa-stGtACR2-FusionRed
Plasmid#105669PurposeExpresses a soma-targeted GtACR2 in mammalian neurons under the CaMKIIa promoterDepositorHas ServiceAAV Retrograde and AAV1InsertstGtACR2
UseAAVTagsFusionRed and Kv2.1ExpressionMammalianPromoterCaMKIIaAvailable SinceFeb. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-GRAB_DA2h
Plasmid#140554PurposeExpresses the 2nd generation high-affinity DA sensor GRAB_DA2h in neuronsDepositorHas ServiceAAV9InsertThe 2nd generation DA sensor GRAB_DA2h (high affinity)
UseAAVPromoterhSynAvailable SinceJuly 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn1-SIO-stGtACR2-FusionRed
Plasmid#105677PurposeExpresses a soma-targeted GtACR2 in Cre-expressing neuronsDepositorHas ServiceAAV Retrograde, AAV1, and AAV5InsertstGtACR2
UseAAVTagsFusionRed and Kv2.1ExpressionMammalianPromoterhSyn1Available SinceFeb. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMOD_B3200
Plasmid#91079PurposeModule B, Promoter: none (begins with P2A to be fused to TALE-VPR_1), Gene: TALE-VPR_2 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSPDepositorInsertTALE-VPR_2 backbone with Esp3I ccdb cassette for repeat cloning
UseTALENPromoternone (begins with P2A to be fused to TALE-VPR_1)Available SinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_B3400
Plasmid#91080PurposeModule B, Promoter: none (begins with P2A to be fused to TALE-VP64_1), Gene: TALE-VP64_2 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSPDepositorInsertTALE-VP64_2 backbone with Esp3I ccdb cassette for repeat cloning
UseTALENPromoternone (begins with P2A to be fused to TALE-VP64_1)Available SinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A3101
Plasmid#91048PurposeModule A, Promoter: 35S, Gene: TALE-VPR_1 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: none (ends with P2A to be fused to TALE-VPR_ 2)DepositorInsertTALE-VPR_1 backbone with Esp3I ccdb cassette for repeat cloning
UseTALENPromoter35SAvailable SinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A3301
Plasmid#91049PurposeModule A, Promoter: 35S, Gene: TALE-VP64_1 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: none (ends with P2A to be fused to TALE-VP64_ 2)DepositorInsertTALE-VP64_1 backbone with Esp3I ccdb cassette for repeat cloning
UseTALENPromoter35SAvailable SinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_B2103b
Plasmid#91062PurposeModule B, Promoter: CmYLCV, Gene: SapI ccdb cassette (doesn't include the promoter) for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35SDepositorInsertSapI ccdb cassette (no promoter) for cloning multiple gRNA spacers with Csy4 spacers
UseCRISPRPromoterCmYLCVAvailable SinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
Perturb-seq GBC library
Pooled Library#85968PurposeBarcode library for cloning gRNA sublibraries for single cell CRISPR analysis.DepositorExpressionMammalianUseCRISPR and LentiviralAvailable SinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hDlx-Flex-GFP-Fishell_6
Plasmid#83895PurposeCre recombinase-dependent GFP expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer elementDepositorHas ServiceAAV Retrograde, AAV1, AAV2, AAV8, and AAV9InserteGFP
UseAAV and Cre/LoxExpressionMammalianPromoterhDlxAvailable SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-S5E2-GFP-fGFP
Plasmid#135631PurposeAAV vector to drive the expression of fGFP in PV cortical interneuronsunder the control of the E2 regulatory elementDepositorHas ServiceAAV1 and AAV9InsertGFP-fGFP
UseAAVMutationNoneAvailable SinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pALuWBB
Plasmid#183202PurposedsRed marked transgene for Crispr co-conversion with w+ gRNA and drop in cloning sites for 2 more gRNADepositorInsertw+ gRNA and scaffold followed by 2 gRNA with BbsI and BsaI cloning sites
UseCRISPR; Recombines at attp landing site in fly ge…ExpressionInsectPromoterU6-3Available SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-S5E2-GCaMP6f
Plasmid#135632PurposeAAV vector to drive the expression of GCaMP6f in PV cortical interneuronsunder the control of the E2 regulatory elementDepositorHas ServiceAAV PHP.eB, AAV1, and AAV9InsertGCaMP6f
UseAAVMutationNoneAvailable SinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-GRAB_rDA1m
Plasmid#140556PurposeExpresses the red fluorescent medium-affinity DA sensor GRAB_rDA1m in neuronsDepositorHas ServiceAAV9InsertRed fluorescent DA sensor GRAB_rDA1m (medium affinity)
UseAAVPromoterhSynAvailable SinceJuly 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST
Plasmid#105448PurposeCation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites in a viral vectorDepositorHas ServiceAAV9InsertChrimsonR-mRuby2-ST
UseAAVTagsmRuby2 and soma targeting motifMutationK176R in ChrimsonPromoterhSynAvailable SinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
JM110
Bacterial Strain#49763PurposeE.coli K-12 derivativeDepositorBacterial ResistanceStreptomycinAvailable SinceOct. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-S5E2-Gq-P2A-dTomato
Plasmid#135635PurposeAAV vector to drive the expression of Gq-DREADD-P2A-dTomato in PV cortical interneuronsunder the control of the E2 regulatory elementDepositorHas ServiceAAV PHP.eB, AAV1, and AAV9InsertGq-P2A-dTomato
UseAAVMutationNoneAvailable SinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only