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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,938 results for: emb

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Showing: 1221 - 1240 of 11938 results

  3. GFP-KIF1B beta-FL

    Plasmid
    #79108
    Purpose
    Mammalian expression of GFP-KIF1B beta-FL
    Depositor
    Susanne Schlisio
    Publication
    Chen et al Cancer Discov. 2014 Apr;4(4):434-51. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GFP-KIF1B beta full length (KIF1B Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    exon 25 missing (natural splice varient)
    Promoter
    CMV
    Available since
    June 14, 2016
    Availability
    Academic Institutions and Nonprofits only

  4. eSpCas9(1.1)-Puro

    Plasmid
    #117686
    Purpose
    The plasmids pSpCas9 (BB)-2A-Puro (Addgene, #48139) and eSpCas9 (1.1) (Addgene, #71814) were combined to express both “enhanced specificity” Cas9 and puromycin resistance protein.
    Depositor
    Luc Leyns
    Publication
    Liu et al Biochem Biophys Res Commun. 2019 Feb 14.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eSpCas9(1.1)
    Use
    CRISPR
    Tags
    PuroR (R166H)
    Expression
    Mammalian
    Mutation
    K848A, K1003A, R1060A
    Promoter
    U6
    Available since
    Feb. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    117686-plasmid-map-sequence-id-236350

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  6. Eef1a-1955/-1>nls::Cas9::nls

    Plasmid
    #59987
    Purpose
    Eef1a (EF1alpha) promoter driving nls::Cas9::nls
    Depositor
    Lionel Christiaen
    Publication
    Stolfi et al Development. 2014 Nov;141(21):4115-20. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nls::Cas9::nls
    Use
    CRISPR
    Mutation
    Reverted mutations from dCas9 to wiltdtype
    Promoter
    Ciinte.Eef1a (EF1alpha) -1955/-1
    Available since
    Nov. 18, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    59987-plasmid-map-sequence-id-95666

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  13. mCherry-APP-mGFP

    Plasmid
    #196704
    Purpose
    Expresses APP695 with two fluorescent proteins at the ends to follow processing in mammalian cells
    Depositor
    Martino Calamai
    Publication
    Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Amyloid Precursor Protein 695 isoform (APP Human)
    Tags
    TagGFP2 and mEGFP
    Expression
    Mammalian
    Available since
    April 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    196704-plasmid-map-sequence-id-386429

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  14. pCAGGS-hPhyB-mCherry-HRasCT

    Plasmid
    #100281
    Purpose
    Expresses Phytochrome B (1-908 aa) fused with mCherry and Hras C-terminus in mammalian cells
    Depositor
    Kazuhiro Aoki
    Publication
    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Phytochrome B (PHYB Mustard Weed)
    Tags
    mCherry fused with HRas C-terminus for plasma mem…
    Expression
    Mammalian
    Mutation
    Human codon optimized PhyB (1-908 aa)
    Promoter
    CAG promoter
    Available since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    100281-plasmid-map-sequence-id-197245

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Showing: 1221 - 1240 of 11938 results