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pMCSG17-NleL

Plasmid

#66716

Purpose

Expresses NleL (170-782) in E. coli

Depositor

David Komander

Publication

Hospenthal et al Nat Struct Mol Biol. 2013 May;20(5):555-

Insert

NleL (NEWENTRY )

Tags

GST-TEV

Expression

Bacterial

Promoter

T7

Available since

July 24, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pcDNA5/FRT/TO V5 DNAJB1

Plasmid

#19522

Depositor

Harm Kampinga

Publication

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DnaJ (Hsp40) homolog, subfamily B, member 1 (DNAJB1 Human)

Tags

V5

Expression

Mammalian

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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mEmerald-WASP1-C-14

Plasmid

#54314

Purpose

Localization: Cytoskeleton, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

WASP1 (WASF1 Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

4 Articles
pCI-OR51E2

Plasmid

#22324

Depositor

Hiroaki Matsunami

Publication

Saito et al Sci Signal. 2009 Mar 3;2(60):ra9. doi: 1

Insert

OR51E2 (OR51E2 Human)

Tags

Rho

Expression

Mammalian

Available since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

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pMRXIP GFP-VAMP8

Plasmid

#45919

Depositor

Noboru Mizushima

Publication

Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

Insert

vesicle-associated membrane protein 8 (VAMP8 Human)

Use

Retroviral

Tags

GFP

Promoter

CMV

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
TAL2472

Plasmid

#36853

Depositor

Keith Joung

Publication

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

TP53-TALEN_Right (Tp53 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2348

Plasmid

#36768

Depositor

Keith Joung

Publication

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

TP53-TALEN_Left (Tp53 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pGEX6p-1-FRB-PafA

Plasmid

#113404

Purpose

Expresses FRB-PafA in bacteria

Depositor

Min Zhuang

Publication

Liu et al Nat Methods. 2018 Aug 13. pii: 10.1038/s

Insert

FRB-PafA

Tags

GST

Expression

Bacterial

Promoter

tac

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX6p-1-BCCP-PupE

Plasmid

#113403

Purpose

Expresses BCCP-PupE in bacteria

Depositor

Min Zhuang

Publication

Liu et al Nat Methods. 2018 Aug 13. pii: 10.1038/s

Insert

BCCP-PupE

Tags

GST

Expression

Bacterial

Promoter

tac

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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FU - dio YFP - W

Plasmid

#73858

Purpose

For expressing YFP in mammalian cells upon Cre co-expression. Can be made into Lentivirus.

Depositor

Elly Nedivi

Publication

Villa et al Neuron. 2016 Feb 3. pii: S0896-6273(16)0

Insert

eYFP

Use

Lentiviral

Expression

Mammalian

Available since

April 29, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.1-alpha4 wt

Plasmid

#80016

Purpose

Mammalian expression of human integrin alpha4

Depositor

Chinten James Lim

Publication

Liu et al Mol Cell Biol. 2013 Nov;33(21):4334-45.

Insert

integrin alpha4 (ITGA4 Human)

Expression

Mammalian

Promoter

CMV

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only
Kif1bβ-pcDNA3

Plasmid

#22534

Depositor

William Kaelin

Publication

Schlisio et al Genes Dev. 2008 Apr 1. 22(7):884-93.

Insert

Kif1bβ (KIF1B Human)

Tags

FLAG

Expression

Mammalian

Mutation

wt cDNA

Available since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA5/FRT/TO V5 DNAJB1

Plasmid

#19522

Depositor

Harm Kampinga

Publication

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DnaJ (Hsp40) homolog, subfamily B, member 1 (DNAJB1 Human)

Tags

V5

Expression

Mammalian

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pSF464

Plasmid

#167672

Purpose

Intermediate vector for four gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

Depositor

Niko Geldner

Publication

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

attL1-ccdB-attL2

Use

CRISPR

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCDH_CMV_TFAM_mKate2

Plasmid

#167331

Purpose

Expresses mKate2 fused to full length TFAM in mammalian cells

Depositor

Tom Misteli

Publication

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Use

Lentiviral

Tags

mKate2

Expression

Mammalian

Promoter

CMV

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-ERT2-iCre-ERT2

Plasmid

#178059

Purpose

Conditionally active form of Cre recombinase (activated in response to tamoxifen) under Syn promoter.

Depositor

Edward Boyden

Publication

Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

ERT2-iCre-ERT2

Use

AAV

Expression

Mammalian

Promoter

Human synapsin 1 (Syn) promoter

Available since

Oct. 9, 2022

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgSLC7A11/xCT-1

Plasmid

#161818

Purpose

knock out SLC7A11/xCT in mammalian cells

Depositor

Boyi Gan

Publication

Liu et al Nat Cell Biol. 2020 Apr;22(4):476-486. d

Insert

SLC7A11 solute carrier family 7 member 11 xCT (SLC7A11 Human)

Use

CRISPR and Lentiviral

Available since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pOPINB-OTUB1*

Plasmid

#65441

Purpose

Expresses activated OTUB1 in E. coli

Depositor

David Komander

Publication

Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

Inserts

UbcH5b (UBE2D2 Human)
otubain-1 (OTUB1 )

Tags

His-3C

Expression

Bacterial

Mutation

Active site cysteine mutated to alanine

Promoter

T7

Available since

July 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles
pcDNA5FRT-EF-Pdgfralpha-EGFPN D842V

Plasmid

#66789

Purpose

Expression of constitutively active murine PDGFRalpha D842V tagged with GFP under the control of EF1a promoter

Depositor

Lotte Pedersen

Publication

Nielsen et al J Cell Sci. 2015 Oct 1;128(19):3543-9. d

Insert

PDGFRalpha (Pdgfra Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

D842V (constitutively active)

Promoter

EF1a

Available since

July 14, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
FLAG-SENP2m

Plasmid

#18713

Depositor

Edward Yeh

Publication

Kang et al Mol Cell. 2010 Apr 23. 38(2):191-201.

Insert

SENP2 (SENP2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

R577L, K578M

Available since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles