We narrowed to 12,421 results for: poly

1. pLV-EF1A-TRIP12-391-938-EGFP-Hygro

   Plasmid

   #238092

   Purpose

   Expression of structurally predicted WWE domain of TRIP12 with EGFP on C terminal

   Depositor

   Robert Sobol

   Article

   Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

   Insert

   TRIP12 (TRIP12 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   aa 319-938 only

   Promoter

   EF1alpha

   Available since

   Aug. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-EF1A-DTX1-1-175-EGFP-Hygro

   Plasmid

   #238093

   Purpose

   Expression of structurally predicted WWE domain of DTX1 with EGFP on C terminal

   Depositor

   Robert Sobol

   Article

   Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

   Insert

   DTX1 (DTX1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   aa 1-175 only

   Promoter

   EF1alpha

   Available since

   Aug. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-EF1A-DTX2-1-178-EGFP-Hygro

   Plasmid

   #238094

   Purpose

   Expression of structurally predicted WWE domain of DTX2 with EGFP on C terminal

   Depositor

   Robert Sobol

   Article

   Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

   Insert

   DTX2 (DTX2 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   aa 1-178 only

   Promoter

   EF1alpha

   Available since

   Aug. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-EF1A-DTX4-1-166-EGFP-Hygro

   Plasmid

   #238095

   Purpose

   Expression of structurally predicted WWE domain of DTX4 with EGFP on C terminal

   Depositor

   Robert Sobol

   Article

   Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

   Insert

   DTX4 (DTX4 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   aa 1-166 only

   Promoter

   EF1alpha

   Available since

   Aug. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-EF1A-HUWE1-1503-1780_EGFP-Hygro

   Plasmid

   #238096

   Purpose

   Expression of structurally predicted WWE domain of HUWE1 with EGFP on C terminal

   Depositor

   Robert Sobol

   Article

   Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

   Insert

   HUWE1 (HUWE1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   aa 1-166 only

   Promoter

   EF1alpha

   Available since

   Aug. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-Hygro-EF1A-Af1521(K35E/Y145R)-myc

   Plasmid

   #196237

   Purpose

   Af1521(K35E/Y145R) macrodomain with a myc tag fused to the C terminus & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

   Insert

   Af1521(K35E/Y145R) encoding a C-terminal myc tag
   

   Use

   Lentiviral

   Tags

   myc tag

   Expression

   Mammalian

   Mutation

   amino acid 35 lysine is replaced with glutamic ac…

   Promoter

   EF1A

   Available since

   Aug. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX-4T-3-GST-Tev-Af1521-myc

   Plasmid

   #196239

   Purpose

   N-terminal GST fusion of Af1521(WT) with a TEV protease site located between the GST tag and Af1521 and a myc tag on the C terminus

   Depositor

   Robert Sobol

   Article

   Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

   Insert

   N-terminal GST fusion of Af1521(WT) with a TEV protease site between GST and Af1521 encoding a C-terminal myc tag
   

   Use

   Tags

   GST tag and myc tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. yTREX-ApR

   Plasmid

   #177307

   Purpose

   genomic integration of genes (inserted in I-SceI-site) via transposon Tn5

   Depositor

   Karl-Erich Jaeger

   Article

   Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

   Type

   Empty backbone

   Use

   Synthetic Biology; Yeast expression, tn5 genomic …

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. ABEmax-Puro V2

   Plasmid

   #226955

   Purpose

   ABEmax plasmid enabling A:T to G:C base editing using a single plasmid.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. DG SaCas9 GFP V3

    Plasmid

    #226961

    Purpose

    CBh-SaCas9-2A-GFP, and 2X hU6-sgRNA (Sa) with BbsI golden gate cloning backbone for dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. SaCas9 GFP V3

    Plasmid

    #226962

    Purpose

    CBh-SaCas9-2A-GFP, and hU6-sgRNA (Sa) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. DG SaCas9 puro V3

    Plasmid

    #226960

    Purpose

    CBh-SaCas9-2A-Puro, and 2X hU6-sgRNA (Sa) with BbsI golden gate cloning backbone for dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. rUBR5_2383-2442_pGEX6P1

    Plasmid

    #209791

    Purpose

    Bacterial expression of UBR5 Mlle domain

    Depositor

    Kalle Gehring

    Article

    Lim et al J Biol Chem. 2006 May 19;281(20):14376-8

    Insert

    Mlle domain of UBR5 (Ubr5 Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 2383-2442 only

    Promoter

    tac

    Available since

    Nov. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. LARP1_323-509

    Plasmid

    #209800

    Purpose

    Bacterial expression of human LARP1 La-module

    Depositor

    Kalle Gehring

    Article

    Kozlov et al Nucleic Acids Res. 2022 Sep 9;50(16):953

    Insert

    LARP1 La-module (LARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 323-509 only

    Promoter

    T7

    Available since

    Nov. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. LARP1_323-410

    Plasmid

    #209801

    Purpose

    Bacterial expression of human LARP1 LaM domain

    Depositor

    Kalle Gehring

    Article

    Kozlov et al Nucleic Acids Res. 2022 Sep 9;50(16):953

    Insert

    LARP1 LaM (LARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 323-410 only

    Promoter

    T7

    Available since

    Nov. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOPINE GFP nanobody:Halo:His6

    Plasmid

    #111090

    Purpose

    Bacterial expression of a fusion protein consisting of a GFP nanobody (Dr. Brett Collins, Addgene plasmid # 49172), the Halo tag and a His6 tag.

    Depositor

    Lennart Wirthmueller

    Article

    Chen et al Front Plant Sci. 2018 Nov 1;9:1581. doi:

    Insert

    GFP Nanobody
    

    Use

    Tags

    Halo and His6

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    T7 promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. PARP1 FL

    Plasmid

    #169815

    Purpose

    Expresses codon optimised full length His tagged PARP1 in bacterial cells

    Depositor

    David Neuhaus

    Article

    Ogden et al Nucleic Acids Res. 2021 Feb 26;49(4):226

    Insert

    Poly[ADP-ribose] polymerase 1 (PARP1 Synthetic, Codon optimised, Human)
    

    Use

    Tags

    MKHHHHHHMKQ

    Expression

    Bacterial

    Mutation

    Valine 762 mutated to an alanine

    Promoter

    T7 promoter

    Available since

    Aug. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. 7Gli:GFP

    Plasmid

    #110494

    Purpose

    reporter of Hedgehog signaling activity

    Depositor

    Michael Lewis

    Article

    Li et al PLoS Genet. 2016 May 19;12(5):e1006054.

    Insert

    7 repeats of the Gli binding site GAACACCCA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    7 repeats of Gli biding site

    Available since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX4T2 RANK-L

    Plasmid

    #108571

    Purpose

    Encodes C-terminal part of RANKL (aa158-aa316) with a GST N-terminal tag

    Depositor

    Jean-Claude Scimeca

    Article

    Beranger et al J Bone Miner Res. 2007 Jul;22(7):975-83.

    Insert

    RANKL (receptor activator of nuclear factor kappa-B ligand) (Tnfsf11 Mouse)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    aa158-aa316

    Promoter

    Ptac (trp/lac)

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. SaCas9v2-Puro

    Plasmid

    #178802

    Purpose

    SaCas9 with 2A-Puro, and a cloning backbone for sgRNA.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh (Cas9-2A Puro) and U6 (gRNA)

    Available since

    Aug. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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