We narrowed to 9,666 results for: poly

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pAMV:nAChRgamma-eGFP

Plasmid

#29693

Depositor

Henry Lester

Article

Pantoja et al Biophys J. 2009 Jan . 96(1):226-37.

Insert

GFP tagged Mus musculus cholinergic receptor, nicotinic, gamma (Chrng Mouse)

Tags

AMV promotor and poly(A) tail

Expression

Bacterial

Mutation

eGFP inserted after position S380

Available Since

July 16, 2011

Availability

Academic Institutions and Nonprofits only

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pUWR-Cad-mTFP

Plasmid

#58327

Purpose

Destination vector for inserting ubi-Cad-mTFP to Drosophila melanogaster genome

Depositor

Denise Montell

Article

Cai et al Cell. 2014 May 22;157(5):1146-59. doi: 1

Insert

Cad-mTFP

Expression

Insect

Promoter

poly-ubiquitin

Available Since

Aug. 5, 2014

Availability

Academic Institutions and Nonprofits only
pCAGGS-C-TEV-HAT

Plasmid

#202597

Purpose

Express N-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cell

Depositor

Chiaki Murakami

Article

Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

Type

Empty backbone

Tags

TEV cleavable site (ENLYFQG) and poly-histidine a…

Expression

Mammalian

Promoter

CAG

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pFSW Syt1-C2A K189-192A IRES GFP

Plasmid

#133823

Purpose

Encodes the C2A domain of synaptotagmin 1 with poly-lysine patch mutations K189-192A for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Insert

Syt1 (Syt1 Rat)

Use

Lentiviral

Tags

IRES-GFP

Mutation

K189-192A

Promoter

hSyn (human synapsin I promoter)

Availability

Academic Institutions and Nonprofits only

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pHCMM3

Plasmid

#167454

Purpose

A control chimera; expresses C. elegans PUP-2 from the Sec63 promoter without the RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (-PAB)]

Depositor

Hugo Cesar Medina-Munoz, Marvin Wickens

Article

Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

PSEC63_yeGFP_cePUP-2_URA3_TADH1

Expression

Yeast

Promoter

PSEC63

Availability

Academic Institutions and Nonprofits only

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c-Flag pcDNA3

Plasmid

#20011

Purpose

This pcDNA3 plasmid contains a new poly-linker and single C-terminal flag-tag sequence for expression in mammalian cells.

Depositor

Stephen Smale

Article

Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.

Has Service

Cloning Grade DNA

Type

Empty backbone

Tags

Flag

Expression

Mammalian

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pG2J

Plasmid

#102882

Purpose

Expresses OsTIR1 via the ADH1 promoter, and mEGFP-AID via the TEF1 promoter in yeast. Both cassettes are integrated in HO locus. Assembly and characterization of the auxin-inducible degradation.

Depositor

Matthias Heinemann

Article

Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

Inserts

OsTIR1
mEGFP-AID

Tags

3xMyc and truncated AID (71-114)

Expression

Yeast

Promoter

ADH1 and TEF1

Available Since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pscAluYNF1

Plasmid

#50934

Purpose

Expresses scAluY RNA in mammalian cells.

Depositor

Katharina Strub

Article

Ivanova et al Nucleic Acids Res. 2015 Mar 11;43(5):287

Insert

scAluYNF1

Expression

Mammalian

Mutation

the poly(A) linker and the right arm of the dimer…

Promoter

7SL RNA promoter (pol III)

Available Since

May 16, 2014

Availability

Academic Institutions and Nonprofits only
pYFP-NEAT1m_v1-RT

Plasmid

#97089

Purpose

Encodes a HR repair template for knock-in a YFP expression cassette downstream of the poly(A) signal of human NEAT1_1 isoform. Best used with px330-NEAT1m_v1 vector.

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

NEAT1m_v1-RT (NEAT1 Human)

Use

CRISPR

Tags

EYFP

Promoter

CMV

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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pYFP-NEAT1_IS_RT

Plasmid

#97091

Purpose

Encodes a HR repair template that mutates the poly(A) signal as well as a few splicing factor binding sites of human NEAT1_1 isoform. Best used with px335-NEAT1_IS_v1 and v2 vectors.

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

NEAT1_IS_RT (NEAT1 Human)

Use

CRISPR

Promoter

CMV

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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pUWR-Cad-Venus

Plasmid

#58328

Purpose

Destination vector for inserting ubi-Cad-Venus to Drosophila melanogaster genome

Depositor

Denise Montell

Article

Cai et al Cell. 2014 May 22;157(5):1146-59. doi: 1

Insert

Cad-Venus

Expression

Insect

Promoter

poly-ubiquitin

Available Since

Aug. 14, 2014

Availability

Academic Institutions and Nonprofits only
pUWR-CadTS

Plasmid

#58325

Purpose

Destination vector for inserting ubi-CadTS to Drosophila melanogaster genome

Depositor

Denise Montell

Article

Cai et al Cell. 2014 May 22;157(5):1146-59. doi: 1

Insert

E-cadherin tension sensor

Expression

Insect

Promoter

poly-ubiquitin

Available Since

Aug. 26, 2014

Availability

Academic Institutions and Nonprofits only
pAMV:nAChRgamma-eGFP

Plasmid

#29693

Depositor

Henry Lester

Article

Pantoja et al Biophys J. 2009 Jan . 96(1):226-37.

Insert

GFP tagged Mus musculus cholinergic receptor, nicotinic, gamma (Chrng Mouse)

Tags

AMV promotor and poly(A) tail

Expression

Bacterial

Mutation

eGFP inserted after position S380

Available Since

July 16, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pUWR-Cad-mTFP

Plasmid

#58327

Purpose

Destination vector for inserting ubi-Cad-mTFP to Drosophila melanogaster genome

Depositor

Denise Montell

Article

Cai et al Cell. 2014 May 22;157(5):1146-59. doi: 1

Insert

Cad-mTFP

Expression

Insect

Promoter

poly-ubiquitin

Available Since

Aug. 5, 2014

Availability

Academic Institutions and Nonprofits only
pTK331

Plasmid

#59572

Purpose

TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP(14) Kozak mKate-G8-L7Ae-PEST 4xFF4 attB2 SGP2(98/30) XbaI attB1 EBFP2 ochre attB2 (insert) AscI (truncated E1) 3'UTR Poly A I-SceI

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP(16) 2xK-turn Kozak EGFP attB2 AscI

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pLV.PARP1#1

Plasmid

#14544

Depositor

Didier Trono

Article

Ariumi et al J Virol. 2005 Mar . 79(5):2973-8.

Insert

PARP1 shRNA (PARP1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

March 27, 2007

Availability

Academic Institutions and Nonprofits only
pLV.PARP1#2

Plasmid

#14545

Depositor

Didier Trono

Article

Ariumi et al J Virol. 2005 Mar . 79(5):2973-8.

Insert

PARP1 shRNA (PARP1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

March 27, 2007

Availability

Academic Institutions and Nonprofits only
pENTR-PCBP1

Plasmid

#16177

Depositor

Huda Zoghbi

Article

Lim et al Cell. 2006 May 19. 125(4):801-14.

Insert

Poly(rC) binding protein 1 (PCBP1 Human)

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pUWR-CadTS-C

Plasmid

#58326

Purpose

Destination vector for inserting ubi-CadTS control to Drosophila melanogaster genome

Depositor

Denise Montell

Article

Cai et al Cell. 2014 May 22;157(5):1146-59. doi: 1

Insert

E-cadherin tension sensor control

Expression

Insect

Promoter

poly-ubiquitin

Available Since

Aug. 5, 2014

Availability

Academic Institutions and Nonprofits only
pLV.PARP1#5

Plasmid

#14548

Depositor

Didier Trono

Article

Ariumi et al J Virol. 2005 Mar . 79(5):2973-8.

Insert

PARP1 shRNA (PARP1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

March 27, 2007

Availability

Academic Institutions and Nonprofits only