We narrowed to 1,827 results for: PORA

1. pAAV-EF1alpha-soCoChR-GFP

   Plasmid

   #107709

   Purpose

   A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. EF1alpha promoter

   Depositor

   Edward Boyden

   Article

   Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

   Insert

   soCoChR-GFP
   

   Use

   AAV

   Tags

   EGFP and KA2

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMRX-IP/SECFP-mATG5

   Plasmid

   #58744

   Purpose

   Expresses /SECFP-ATG5 in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

   Insert

   autophagy related genes 5 (Atg5 Mouse)
   

   Use

   Retroviral

   Tags

   SECFP

   Expression

   Mutation

   Promoter

   Available since

   Aug. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pBEST-p70a-UTR1-Nterm 6xHis PL-T500

   Plasmid

   #92314

   Purpose

   Expression of NTerm 6xHis tagged protein L (PL) domain using the p70a promoter and UTR1

   Depositor

   Lital Alfonta

   Article

   Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

   Insert

   B1 domain of protein L (PL)
   

   Use

   Synthetic Biology; Cell free protein synthesis (c…

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   p70a

   Available since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSF3-Flag-Cbir-Cdc25C S216A_Myc-Nbir-14-3-3e

   Plasmid

   #90011

   Purpose

   Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C S216A and N-terminally tagged NBir[2-256]-14-3-3e

   Depositor

   Julien Béthune

   Article

   Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

   Inserts

   CBirA*-Cdc25C S216A
   NBirA*-14-3-3e
   

   Use

   Retroviral; Flp/frt

   Tags

   FLAG and Myc

   Expression

   Mammalian

   Mutation

   Ser 216 to Ala

   Promoter

   CMV

   Available since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. FKBP-GTSE1(638-720)-GFP

   Plasmid

   #100735

   Purpose

   To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

   Depositor

   Stephen Royle

   Article

   Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

   Insert

   GTSE1 (GTSE1 Human)
   

   Use

   Tags

   FKBP and GFP

   Expression

   Mammalian

   Mutation

   C-terminus only

   Promoter

   CMV

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBEST-p70b-UTR1-deGFP-6xHis-T500

   Plasmid

   #92222

   Purpose

   Expression of deGFP using the p70b promoter (weak version of the p70a promoter)

   Depositor

   Lital Alfonta

   Article

   Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

   Insert

   deGFP
   

   Use

   Synthetic Biology; Cell free protein synthesis (c…

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   p70b (p70a promoter with one mutation)

   Available since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAS_4xG1 PylT A41AA C55A sfGFP 150TAG

   Plasmid

   #154770

   Purpose

   plasmid with 4xG1 PylT cassette (G1 PylT mutant A41AA C55A) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   150TAG in GFP reporter, G1 PylT with A41AA and C5…

   Promoter

   EF1

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pInducer20 2xHA HCAR3

   Plasmid

   #226811

   Purpose

   Tet-inducible lentiviral vector for 2xHA HCAR3 expression

   Depositor

   Ramiro Iglesias-Bartolome

   Article

   Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

   Insert

   HCAR3 (HCAR3 Human)
   

   Use

   Lentiviral

   Tags

   2xHA tag followed by a GDPPVAT linker

   Expression

   Mutation

   Sequence optimized from NP_006009.2

   Promoter

   Available since

   May 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. FUGW-RUSH-LAMP1-V5-APEX2

   Plasmid

   #228524

   Purpose

   Lentiviral vector, expresses Strep-KDEL-IRES-SBP-(Rat)LAMP1-V5-APEX2 in mammalian cells.

   Depositor

   Ginny Farias

   Article

   Li et al Nat Commun. 2024 Dec;15: 10829. doi: 10.

   Insert

   LAMP1 (Lamp1 Rat)
   

   Use

   Lentiviral

   Tags

   Streptavidin Binding Protein (SBP), Streptavidin-…

   Expression

   Mammalian

   Mutation

   Promoter

   Ubc

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. FUGW-RUSH-LAMP1-mScarleti

    Plasmid

    #228526

    Purpose

    Lentiviral vector, expresses Strep-KDEL-IRES-SBP-(Rat)LAMP1-mScarleti in mammalian cells.

    Depositor

    Ginny Farias

    Article

    Li et al Nat Commun. 2024 Dec;15: 10829. doi: 10.

    Insert

    LAMP1 (Lamp1 Rat)
    

    Use

    Lentiviral

    Tags

    Streptavidin Binding Protein (SBP), Streptavidin-…

    Expression

    Mammalian

    Mutation

    Promoter

    Ubc

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKT2/CAGXSP/GFPCD63

    Plasmid

    #231709

    Purpose

    Stably express CD63 fused with GFP in mammalian cells via the sleeping beauty transposon system.

    Depositor

    Masamitsu Kanada

    Article

    Li et al Sci Rep. 2024 Nov 9;14(1):27432. doi: 10

    Insert

    CD63 (CD63 Human)
    

    Use

    Tags

    gfp

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pENTR1A 2xHA HCAR3

    Plasmid

    #226807

    Purpose

    Gateway entry vector for 2xHA HCAR3

    Depositor

    Ramiro Iglesias-Bartolome

    Article

    Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

    Insert

    HCAR3 (HCAR3 Human)
    

    Use

    Gateway entry

    Tags

    2xHA tag followed by a GDPPVAT linker

    Expression

    Mutation

    Sequence optimized from NP_006009.2

    Promoter

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAcBac1-Pyl-TAG

    Plasmid

    #228784

    Purpose

    Pyl aaRS/tRNA system for mammalian TAG suppression

    Depositor

    Abhishek Chatterjee

    Article

    Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

    Inserts

    PylRS
    PytR
    

    Use

    Baculoviral

    Tags

    Expression

    Mammalian

    Mutation

    TAG suppressor

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAcBac1-Pyl-TAA

    Plasmid

    #228783

    Purpose

    Pyl aaRS/tRNA system for mammalian TAA suppression

    Depositor

    Abhishek Chatterjee

    Article

    Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

    Inserts

    PylRS
    PytR
    

    Use

    Baculoviral

    Tags

    Expression

    Mammalian

    Mutation

    TAA suppressor

    Promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA-MYC-As-NF-kB3

    Plasmid

    #180205

    Purpose

    Expresses MYC-tagged Acanthoeca spectabilis NF-kB3 protein in mammalian cells

    Depositor

    Thomas Gilmore

    Article

    Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

    Insert

    As-NF-kB3
    

    Use

    Tags

    MYC-tagged fusion protein

    Expression

    Mammalian

    Mutation

    aa 2-412

    Promoter

    CMV

    Available since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. SSH-pcDNA3.1-SSH-SARS-CoV-2-S-WT

    Plasmid

    #218460

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 spike (1273 residues)

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

    Insert

    SARS-CoV-2 spike protein (1273 residues)-wild type (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA-FLAG-As-NF-kB2

    Plasmid

    #180201

    Purpose

    Expresses FLAG-tagged Acanthoeca spectabilis NF-kB2 protein in mammalian cells

    Depositor

    Thomas Gilmore

    Article

    Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

    Insert

    As-NF-kB2
    

    Use

    Tags

    FLAG-tagged fusion protein

    Expression

    Mammalian

    Mutation

    aa 2-502

    Promoter

    CMV

    Available since

    June 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. H2B-GFP-INCENP∆cen

    Plasmid

    #184044

    Purpose

    Expresses H2B-GFP fused to INCENP∆cen

    Depositor

    Lienhard Schmitz

    Article

    Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

    Insert

    INCENP (INCENP Human)
    

    Use

    Tags

    GFP and H2B

    Expression

    Mammalian

    Mutation

    Truncated to 1-862

    Promoter

    CMV

    Available since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT2/shCol1a1/Scarlet_Seq1.2

    Plasmid

    #201404

    Purpose

    Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Article

    Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

    Insert

    COL1A1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pT2/shCol1a1/Scarlet-Seq1.1

    Plasmid

    #201401

    Purpose

    Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Article

    Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

    Insert

    COL1A1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge