168,238 results

1. Keasling-2337

   Plasmid

   #100168

   Purpose

   Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth

   Depositor

   Jay Keasling

   Article

   Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.

   Insert

   lacIq-Ptrc-ADS
   

   Expression

   Bacterial

   Promoter

   trc

   Available Since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR221_SLC15A4

   Plasmid

   #131910

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC15A4 (SLC15A4 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 11, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. hGli3 flag3x

   Plasmid

   #84921

   Purpose

   mammalian expression of Gli3

   Depositor

   Martin Fernandez-Zapico

   Insert

   Gli3 (GLI3 Human)
   

   Tags

   3xFlag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-GRAB_r5-HT1.0

   Plasmid

   #208719

   Purpose

   Expresses the red 5-HT sensor GRAB_r5-HT1.0 in neurons

   Depositor

   Yulong Li

   Article

   Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

   Insert

   Red fluorescent 5-HT sensor GRAB_r5-HT1.0
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV2-DEST(r)

   Plasmid

   #190238

   Purpose

   Gateway destination vector, empty AAV vector backbone

   Depositor

   Hiroyuki Hioki

   Article

   Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

   Type

   Empty backbone

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.3_SARS2_JN.1

   Plasmid

   #218678

   Purpose

   expressing SARS-CoV-2 JN.1 spike protein for pseudovirus production

   Depositor

   David Nemazee

   Insert

   Spike (JN.1)
   

   Expression

   Mammalian

   Mutation

   L455S on the basis of BA.2.86

   Promoter

   CMV

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-ER-mRFP

   Plasmid

   #231180

   Purpose

   Expresses control plasmid for ER-mitochondrial linkers, tagged with mRFP, from a lentiviral vector

   Depositor

   Dmitry Lim

   Article

   Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

   Insert

   ER-mRFP
   

   Use

   Lentiviral

   Promoter

   CMV

   Available Since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYPQ-Cas9-Act3.0

   Plasmid

   #178954

   Purpose

   It consists of a catalytically active Cas9 nuclease and MS2-SunTag-activators activation complex (ScFv-sfGFP-2xTAD), enabling simultaneous genome editing and gene activation.

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

   Insert

   zCas9-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRVL-2

   Plasmid

   #104580

   Purpose

   Contains mouse IgG2c CH1/CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Full Fc-mediated immune effector functions

   Depositor

   Daniel Christ

   Article

   Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi

   Insert

   Mouse IgG2c CH1/CH2/CH3
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. GABA(A) receptor subunit a1SE

    Plasmid

    #49168

    Purpose

    pHluorin-tagged GABA A receptor subunit (alpha 1) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface

    Depositor

    Tija Jacob, Stephen Moss

    Article

    Tretter et al J Neurosci. 2008 Feb 6;28(6):1356-65. do

    Insert

    GABA(A) receptor subunit alpha-1 (Gabra1 Mouse)
    

    Tags

    myc and pHGFP (pHIuorin)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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