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pcDNA3 GATA1

Plasmid

#85693

Purpose

Mammalian expression of mouse GATA1

Depositor

Licio Collavin, Giannino Del Sal

Article

Collavin et al Proc Natl Acad Sci U S A. 2004 Jun 15;10

Insert

GATA1 (Gata1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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PRS_8-GCaMP8m

Plasmid

#210400

Purpose

AAV-vector for fluorescent calcium measurements in the green spectrum from noradrenergic neurons

Depositor

Simon Wiegert

Article

Wissing et al PLoS Biol. 2025 Jul 7;23(7):e3003228. do

Has Service

AAV2

Insert

GCaMP8m

Use

AAV

Expression

Mammalian

Promoter

PRSx8

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pTK331

Plasmid

#59572

Purpose

TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP(14) Kozak mKate-G8-L7Ae-PEST 4xFF4 attB2 SGP2(98/30) XbaI attB1 EBFP2 ochre attB2 (insert) AscI (truncated E1) 3'UTR Poly A I-SceI

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP(16) 2xK-turn Kozak EGFP attB2 AscI

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaM-3

Plasmid

#172769

Purpose

Bacterial expression of anti-mCherry nanobody LaM-3, with pelB leader and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaM-3

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pAAP2

Plasmid

#73211

Purpose

express Assembly-activating protein (AAP) of adeno-associated virus serotype 2 (AAV2)

Depositor

Luk Vandenberghe

Article

Zinn et al Cell Rep. 2015 Jul 28. pii: S2211-1247(1

Insert

AAP2

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pLEX301-TagRFP

Plasmid

#162035

Purpose

Tagged vector control

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Type

Empty backbone

Use

Lentiviral

Tags

RFP

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-WIPI3

Plasmid

#42527

Depositor

James Hurley

Article

Baskaran et al Mol Cell. 2012 Aug 10;47(3):339-48. doi:

Insert

WIPI3 (WDR45B Human)

Tags

GST-TEV

Expression

Mammalian

Mutation

gene sequence is codon optimized for expression i…

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pCMV-mFlagEts1

Plasmid

#86099

Purpose

expresses flag tagged Ets1 in mammalian cells

Depositor

Barbara Graves

Article

Seidel et al Genes Dev. 2002 Jan 1;16(1):127-37.

Insert

Ets1 (Ets1 Mouse)

Tags

Flag tag; kanamycin selection

Expression

Mammalian

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-TIP49b

Plasmid

#15358

Depositor

Joan Conaway, Ronald Conaway

Article

Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

Insert

RuvB-like 2 (RUVBL2 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-16-4GS-LaG-2

Plasmid

#172764

Purpose

Bacterial expression of dimerized anti-GFP nanobodies LaG-16 and LaG-2 (4GS linker)

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-16-4GS-LaG-2

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-H3.3-N-14

Plasmid

#55302

Purpose

Localization: Nucleus/Histones, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

H3.3 (H3f3b Mouse)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pUC19-U6-LwaCas13acrRNA-BsmBIcassette (LTH151)

Plasmid

#171129

Purpose

LwaCas13a crRNA entry vector for pU6 expression (need to clone in spacer into BsmBI sites)

Depositor

Benjamin Kleinstiver

Article

Johnson et al Mol Cell. 2022 Jun 2;82(11):2161-2166.e3

Insert

U6 promoter entry plasmid for LwaCas13a crRNA cloning

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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AviTag-SpyTag003-MBP

Plasmid

#184227

Purpose

Expresses AviTag and SpyTag003 linked to maltose binding protein in the bacterial cytoplasm. AviTag enables site-specific biotinylation by BirA. SpyTag003 enables reaction with SpyCatcher003.

Depositor

Mark Howarth

Article

Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

Insert

AviTag-SpyTag003-MBP

Tags

AviTag, His6, SpyTag003, and Thrombin cleavage si…

Expression

Bacterial

Promoter

T7

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-16-3xFLAG-LaG-2

Plasmid

#172765

Purpose

Bacterial expression of dimerized anti-GFP nanobodies LaG-16 and LaG-2 (3xFLAG linker)

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-16-3xFLAG-LaG-2

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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DsRed-p150 217–548

Plasmid

#51146

Purpose

Inhibits dynein-dynactin interaction in mammalian cells

Depositor

Trina Schroer

Article

Quintyne et al J Cell Biol. 2002 Oct 28;159(2):245-54.

Insert

DCTN1 (DCTN1 Chicken)

Expression

Mammalian

Mutation

Q118R, E340K

Promoter

CMV

Available Since

March 28, 2017

Availability

Academic Institutions and Nonprofits only
pHAGE-EF1a-HSF1-GFP

Plasmid

#227820

Purpose

Expresses HSF1 fused to GFP from EF1a promoter

Depositor

Jeffrey Gerst

Article

Pataki et al Sci Adv. 2025 Dec 5;11(49):eadu0315. doi

Insert

HSF1 (HSF1 Human)

Use

Lentiviral

Tags

3xHA and GFP

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pBABE HSF Flag wt

Plasmid

#1948

Depositor

Robert Kingston

Article

Sullivan et al Mol Cell Biol 2001 Sep;21(17):5826-37.

Insert

Heat Shock Factor 1 (HSF1 Human)

Use

Retroviral

Tags

flag

Expression

Mammalian

Available Since

March 23, 2006

Availability

Academic Institutions and Nonprofits only

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pLEX303-GFP

Plasmid

#162032

Purpose

Tagged vector control

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Type

Empty backbone

Use

Lentiviral

Tags

GFP

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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mCherry-INPP5E

Plasmid

#171948

Purpose

Expresses INPP5E in mammalian cells

Depositor

Cheng-han Yu

Article

Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

INPP5E (INPP5E Human)

Tags

mCherry

Expression

Mammalian

Available Since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pVEX-CC1

Plasmid

#74170

Purpose

protein expression of chicken DCTN1 p150Glued AA 217-548 (CC1) in bacteria

Depositor

Trina Schroer

Article

Quintyne et al J Cell Biol. 1999 Oct 18;147(2):321-34.

Insert

DCTN1 (DCTN1 Chicken)

Tags

None

Expression

Bacterial

Promoter

Inducible Tac promoter

Available Since

May 9, 2016

Availability

Academic Institutions and Nonprofits only