We narrowed to 1,605 results for: N-pac

1. sgRNA(MS2) cloning backbone

   Plasmid

   #61424

   Purpose

   sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. SP6-VEE-IRES-Puro

   Plasmid

   #58971

   Purpose

   noninfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

   Depositor

   Steven Dowdy

   Article

   Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

   Type

   Empty backbone

   Use

   Venezuelan equine encephalitis (vee) virus rna re…

   Expression

   Mammalian

   Promoter

   26S subgenomic promoter

   Available Since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. tetO-NKX2.5

   Plasmid

   #46029

   Depositor

   John Gearhart

   Article

   Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

   Insert

   NKX2.5 (NKX2-5 Human)
   

   Use

   Lentiviral

   Tags

   His and V5

   Expression

   Mammalian

   Promoter

   tetO

   Available Since

   July 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMSCV-FKBP-SYNDAPIN2

   Plasmid

   #221311

   Purpose

   Expression of SYNDAPIN2 (pLxIS) in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

   Insert

   SYNDAPIN2 (pLxIS) (Pacsin2 Mouse)
   

   Use

   Retroviral

   Tags

   FLAG-4xFKBP

   Available Since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKD280

   Plasmid

   #125071

   Purpose

   Expression of SO_4387 under Ptac

   Depositor

   Jeffrey Tabor

   Article

   Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

   Inserts

   SO_4387 (SO_4387 )
   lacI (lacI )
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   S286L- Please see depositor comments below

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. cACTB

   Plasmid

   #236544

   Purpose

   For expression of ACTB in mammalian cells

   Depositor

   Sandra Citi

   Article

   Mauperin et al Nat Commun. 2025 Mar 13;16(1):2514. doi:

   Insert

   ACTB
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   hpACTB

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKD286

   Plasmid

   #125074

   Purpose

   Expression of SO_4622 under Ptac

   Depositor

   Jeffrey Tabor

   Article

   Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

   Inserts

   SO_4622 (SO_4622 )
   lacI (lacI )
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   S286L- Please see depositor comments below

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKD276

   Plasmid

   #125069

   Purpose

   Expression of SO_0621 under Ptac

   Depositor

   Jeffrey Tabor

   Article

   Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

   Inserts

   SO_0621 (SO_0621 )
   lacI (lacI )
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   S286L- Please see depositor comments below

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. SK-YFP-ST1-A

   Plasmid

   #48665

   Purpose

   Bacterial ST1 repression YFP reporter: protospacer A

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Insert

   ST1 prototspacer A/PAM with reporter EYFP
   

   Use

   CRISPR

   Promoter

   lambda pR promoter

   Available Since

   Oct. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. PM-ST1!TB

    Plasmid

    #48654

    Purpose

    Bacterial ST1 crRNA expression: targets ST1 to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    Bacterial ST1 crRNA to prototspacer B
    

    Use

    CRISPR

    Promoter

    J23100 promoter

    Available Since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. SK-YFP-ST1-B

    Plasmid

    #48662

    Purpose

    Bacterial ST1 repression YFP reporter: protospacer B

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    ST1 prototspacer B/PAM with reporter EYFP
    

    Use

    CRISPR

    Promoter

    lambda pR promoter

    Available Since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. PM-ST1!TA

    Plasmid

    #48653

    Purpose

    Bacterial ST1 crRNA expression: targets ST1 to protospacer A (TACCATCTCAAGCTTGTTGA), p15A/chloramphenicol

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    Bacterial ST1 crRNA to prototspacer A
    

    Use

    CRISPR

    Promoter

    J23100 promoter

    Available Since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. mTB3-BC2 nanobody

    Plasmid

    #210208

    Purpose

    Type 3 phage-display vector that contains kanamycin resistance and displays the BC2 nanobody at the N-terminus of mature protein III.

    Depositor

    Brian Kay

    Article

    Grahn et al Methods Mol Biol. 2023;2702:191-203. doi

    Insert

    BC2 Nanobody
    

    Expression

    Bacterial

    Available Since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pL8N_RB610-HBB_X1

    Plasmid

    #191521

    Purpose

    Expression of nuclear ZF-DdCBE in mammalian cells (N-terminal DddAC, Right)

    Depositor

    David Liu

    Article

    Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

    Insert

    ZF-DdCBE RB610-HBB X1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pL5N_LT32-HBB_X1

    Plasmid

    #191520

    Purpose

    Expression of nuclear ZF-DdCBE in mammalian cells (N-terminal DddAN, Left)

    Depositor

    David Liu

    Article

    Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

    Insert

    ZF-DdCBE LT32-HBB X1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEC3109 (pSpy1C-Plac(1)_ffluc)

    Plasmid

    #218514

    Purpose

    replicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressor

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Insert

    ffluc
    

    Use

    Luciferase

    Expression

    Bacterial

    Mutation

    deletion of mrfp cassette, mutagenesis of the RBS…

    Promoter

    Plac(1)

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEC3111 (pSpy1C-Plac(3)_ffluc)

    Plasmid

    #218778

    Purpose

    replicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressor

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Insert

    ffluc
    

    Use

    Luciferase

    Expression

    Bacterial

    Mutation

    deletion of mrfp cassette, mutagenesis of the RBS…

    Promoter

    Plac(3)

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pZDonor-Nm-Cas9-gRNA-hU6

    Plasmid

    #61366

    Purpose

    empty backbone for cloning N. meningiditis protospacers. Encodes constant region of N. meningiditis Cas9 synthetic gRNA

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. cACTG1

    Plasmid

    #236543

    Purpose

    For expression of ACTG1 in mammalian cells

    Depositor

    Sandra Citi

    Article

    Mauperin et al Nat Commun. 2025 Mar 13;16(1):2514. doi:

    Insert

    ACTG1
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    hpACTB

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGG422_UV5

    Plasmid

    #165607

    Purpose

    Vector for expression of the SpCas9 KG variant with sgRNA in E. coli: KG(SpCas9, D1332K/R1333G) and UV5-sgRNA (hEGFP spacer)

    Depositor

    Marcus Noyes

    Article

    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

    Insert

    lacUV5 driving Streptococcus pyogenes Cas9 KG(D1332K/R1333G) and hEGFP-sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Mutation

    D1332K and R1333G mutations in SpCas9

    Promoter

    lacUV5 driving Cas9 KG and UV5 driving sgRNA

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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