We narrowed to 1,335 results for: piggyBac

1. pB-Halo,IRES-NGFR

   Plasmid

   #164520

   Purpose

   All-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal Halo tagged protein (inducible NGFR and constitutive rtTA and neomycin resistance)

   Depositor

   Mitchell Guttman

   Article

   Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-CuO-V5-CASC3 siRes 110-480 188/218-mut

   Plasmid

   #158544

   Purpose

   For PiggyBac-mediated integration of V5-tagged siRNA-resistant EJC-binding deficient CASC3 110-480

   Depositor

   Niels Gehring

   Article

   Gerbracht et al Nucleic Acids Res. 2020 Jul 4. pii: 5867

   Insert

   CASC3; BTZ; MLN51 (CASC3 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Amino acids 110-480, C-terminal deletion, F188D, …

   Promoter

   CMV

   Available Since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. PBbtCRISPRko.v1

   Pooled Library

   #213928

   Purpose

   Uses the PiggyBac transposon system to deliver the library into hard to transduce cells

   Depositor

   Wenfang Spring Tan

   Article

   Tan et al PLoS Pathog. 2023 Dec 6;19(12):e1011822.

   Expression

   Mammalian

   Use

   CRISPR and Lentiviral

   Available Since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

4. epB_UbC_CymRV5-nls-GFP_DEx2

   Plasmid

   #119906

   Purpose

   ePiggyBac mammalian expression plasmid for CymR-GFP fusion

   Depositor

   Orion Weiner

   Article

   Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

   Insert

   CymRV5-nls-GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. epB_CAG_TetRFlag-nls-GFPx2_DEx2

   Plasmid

   #119910

   Purpose

   ePiggyBac mammalian expression plasmid for TetR-GFP fusion

   Depositor

   Orion Weiner

   Article

   Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

   Insert

   TetRFlag-nls-GFPx2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. epB_UbC_CymRV5-nls-Halox2_DEx4

   Plasmid

   #119907

   Purpose

   ePiggyBac mammalian expression plasmid for CymR-Halo fusion

   Depositor

   Orion Weiner

   Article

   Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

   Insert

   CymRV5-nls-Halox2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. epB_CAG_TetRFlag-nls-tdTom_DEx4

   Plasmid

   #119909

   Purpose

   ePiggyBac mammalian expression plasmid for TetR-tdTom fusion

   Depositor

   Orion Weiner

   Article

   Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

   Insert

   TetRFlag-nls-tdTom
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. epB_UbC_tdMS2cp-tagRFP-Tx2

   Plasmid

   #119908

   Purpose

   ePiggyBac mammalian expression plasmid for tandem dimer MS2cp-tagRFP-T fusion

   Depositor

   Orion Weiner

   Article

   Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

   Insert

   HA-tdMS2cp-tagRFP-Tx2-nls
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pB-6HIS-TEV-mEGFP,IRES-NGFR

   Plasmid

   #164521

   Purpose

   All-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal His-TEV-GFP tagged protein (inducible NGFR and constitutive rtTA and neomycin resistance)

   Depositor

   Mitchell Guttman

   Article

   Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB-U6-sgRNA-BFP-Puro

    Plasmid

    #229013

    Purpose

    Perturb Seq sgRNA vector with PiggyBac backbone

    Depositor

    Gary Hon

    Article

    Sivakumar et al bioRxiv [Preprint]. 2025 Jan 23:2025.01.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCBh_3x Flag-NLS-denOsCas12f1-NLS-VPR-NLS-mCherry_pA_phU6_gRNA scaffold-spacer_pCMV_BFP_pA

    Plasmid

    #197031

    Purpose

    Vector encoding a human codon-optimized dead enOsCas12f1 (denOsCas12f1)-VPR driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and BFP driven by CMV promoter

    Depositor

    Xiangfeng Kong

    Article

    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

    Insert

    humanized denOsCas12f1-VPR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D52R / T132R / D228A / D406A

    Promoter

    CBh, CMV, hU6

    Available Since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pB-Tet-On-HTR6-RhoA sensor

    Plasmid

    #189614

    Purpose

    Tet-On cilia-targeted RhoA sensor (Xlone piggybac back-bone); HTR6-fused with a sGFP-mScarlet-I based sensor

    Depositor

    David Clapham

    Article

    Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

    Insert

    HTR6 (HTR6 Human)
    

    Use

    Tags

    GFP and mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    Tet3G

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPB-TRE-V5-TurboID-linker-T2A-GR-bGH polyA > mPGK-BlastR-SV40 polyA

    Plasmid

    #218910

    Purpose

    PiggyBac transposition

    Depositor

    Sven Eyckerman

    Article

    Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    V5-TurboID-T2A-huGR/BlastR (NR3C1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    2363G>C and 4155C>T and 4729T>G and 4731…

    Promoter

    Available Since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTR01F/hFGFR3b-V5

    Plasmid

    #236016

    Purpose

    for PiggyBac mediated integration and stable expression of hFGFR3b protein

    Depositor

    Pavel Krejčí

    Article

    Zlinska et al Mol Ther Nucleic Acids. 2024 Nov 28;36(1

    Insert

    human FGFR3b (FGFR3 Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    Promoter

    truncated CMV promoter

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTR01F/hFGFR4-V5

    Plasmid

    #236017

    Purpose

    for PiggyBac mediated integration and stable expression of hFGFR4 protein

    Depositor

    Pavel Krejčí

    Article

    Zlinska et al Mol Ther Nucleic Acids. 2024 Nov 28;36(1

    Insert

    human FGFR4 (FGFR4 Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    Promoter

    truncated CMV promoter

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. TR01F/hFGFR3-G380R-V5

    Plasmid

    #214908

    Purpose

    for PiggyBac mediated integration and stable expression of hFGFR3-G380R variant that is associated with Achondroplasia

    Depositor

    Pavel Krejčí

    Article

    Kimura et al Sci Transl Med. 2021 May 5;13(592):eaba4

    Insert

    FGFR3 (FGFR3 Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    G380R substitution

    Promoter

    truncated CMV promoter

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCBh-NLS_hfCas13d(RfxCas13d_N2V8)_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid

    #190034

    Purpose

    vector for encoding a human codon-optimized High-fidelity RfxCas13d (hfCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP and mCherry driven by SV40 promoters

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

    Insert

    humanized hfCas13d
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A134V,A140V,A141V,A143V

    Promoter

    CBh, SV40, hU6

    Available Since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pXJ219-pAAV-PB3'IR-U6-gRNA-EF1a-mtagBFP2-KASH-PB5'IR

    Plasmid

    #210525

    Purpose

    AAV piggybac transposon vector, with mtagBFP-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.

    Depositor

    Xin Jin

    Article

    Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

    Inserts

    mtagBFP-KASH
    U6-gRNA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pXJ385-pAAV-PB3’IR-U6-gRNA-EF1a-mScarlet-KASH-PB5’IR

    Plasmid

    #224435

    Purpose

    AAV piggybac transposon vector, with mScarlet-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.

    Depositor

    Xin Jin

    Article

    Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

    Inserts

    mScarlet-KASH
    U6-gRNA
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pXJ220-pAAV-PB3'IR-U6-gRNA-EF1a-GFP-KASH-PB5'IR

    Plasmid

    #213481

    Purpose

    AAV piggybac transposon vector, with GFP-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.

    Depositor

    Xin Jin

    Article

    Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

    Inserts

    GFP-KASH
    U6-gRNA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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